Publicação
Injectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defects
| Resumo: | In this work, the ability of gellan gum hydrogels coupled with autologous cells to regenerate rabbit full-thickness articular cartilage defects was tested. Five study groups were defined: (a) gellangumwith encapsulated chondrogenic predifferentiated rabbit adipose stem cells (ASCþGF); (b) gellan gum with encapsulated nonchondrogenic predifferentiated rabbit adipose stem cells (ASC); (c) gellan gum with encapsulated rabbit articular chondrocytes (AC) (standard control); (d) gellan gum alone (control); (e) empty defect (control). Fullthickness articular cartilage defects were created and the gellangum constructs were injected and left for 8 weeks. The macroscopic aspect of the explants showed a progressive increase of similarity with the lateral native cartilage, stable integration at the defect site, more pronouncedly in the cell-loaded constructs. Tissue scoring showed that ASCþGF exhibited the best results regarding tissue quality progression. Alcian blue retrieved similar results with a better outcome for the cell-loaded constructs. Regarding real-time PCR analyses, ASCþGF had the best progression with an upregulation of collagen type II and aggrecan, and a downregulation of collagen type I. Gellan gum hydrogels combined with autologous cells constitute a promising approach for the treatment of articular cartilage defects, and adipose derived cellsmayconstitute a valid alternative to currently used articular chondrocytes. |
|---|---|
| Autores principais: | Oliveira, J. T. |
| Outros Autores: | Gardel, Leandro. S.; Rada, Tommaso; Martins, L.; Gomes, Manuela E.; Reis, R. L. |
| Assunto: | Gellan gum Stem cells Cartilage Tissue engineering In vivo |
| Ano: | 2010 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439687194378240 |
|---|---|
| author | Oliveira, J. T. |
| author2 | Gardel, Leandro. S. Rada, Tommaso Martins, L. Gomes, Manuela E. Reis, R. L. |
| author2_role | author author author author author |
| author_facet | Oliveira, J. T. Gardel, Leandro. S. Rada, Tommaso Martins, L. Gomes, Manuela E. Reis, R. L. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Oliveira, J. T.\"},{\"Person.name\":\"Gardel, Leandro. S.\"},{\"Person.name\":\"Rada, Tommaso\"},{\"Person.name\":\"Martins, L.\"},{\"Person.name\":\"Gomes, Manuela E.\"},{\"Person.name\":\"Reis, R. L.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Oliveira, J. T. Gardel, Leandro. S. Rada, Tommaso Martins, L. Gomes, Manuela E. Reis, R. L. |
| datacite.date.Accepted.fl_str_mv | 2010-09-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2012-10-29T18:32:16Z |
| datacite.date.embargoed.fl_str_mv | 2012-10-29T18:32:16Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Gellan gum Stem cells Cartilage Tissue engineering In vivo |
| datacite.titles.title.fl_str_mv | Injectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defects |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Oliveira, J. T. Gardel, Leandro. S. Rada, Tommaso Martins, L. Gomes, Manuela E. Reis, R. L. |
| dc.date.Accepted.fl_str_mv | 2010-09-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2012-10-29T18:32:16Z |
| dc.date.embargoed.fl_str_mv | 2012-10-29T18:32:16Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/20590 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Wiley |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Gellan gum Stem cells Cartilage Tissue engineering In vivo |
| dc.title.fl_str_mv | Injectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defects |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | In this work, the ability of gellan gum hydrogels coupled with autologous cells to regenerate rabbit full-thickness articular cartilage defects was tested. Five study groups were defined: (a) gellangumwith encapsulated chondrogenic predifferentiated rabbit adipose stem cells (ASCþGF); (b) gellan gum with encapsulated nonchondrogenic predifferentiated rabbit adipose stem cells (ASC); (c) gellan gum with encapsulated rabbit articular chondrocytes (AC) (standard control); (d) gellan gum alone (control); (e) empty defect (control). Fullthickness articular cartilage defects were created and the gellangum constructs were injected and left for 8 weeks. The macroscopic aspect of the explants showed a progressive increase of similarity with the lateral native cartilage, stable integration at the defect site, more pronouncedly in the cell-loaded constructs. Tissue scoring showed that ASCþGF exhibited the best results regarding tissue quality progression. Alcian blue retrieved similar results with a better outcome for the cell-loaded constructs. Regarding real-time PCR analyses, ASCþGF had the best progression with an upregulation of collagen type II and aggrecan, and a downregulation of collagen type I. Gellan gum hydrogels combined with autologous cells constitute a promising approach for the treatment of articular cartilage defects, and adipose derived cellsmayconstitute a valid alternative to currently used articular chondrocytes. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/c34af55c-e9ad-424e-bfbb-76e7eee34564/download |
| id | rum_6411bc331f03b3be73fb83a7ea3dbe9b |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/20590 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/20590 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Oliveira, J. T. Gardel, Leandro. S. Rada, Tommaso Martins, L. Gomes, Manuela E. Reis, R. L. |
| publishDate | 2010 |
| publisher.none.fl_str_mv | Wiley |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engWileyporIn this work, the ability of gellan gum hydrogels coupled with autologous cells to regenerate rabbit full-thickness articular cartilage defects was tested. Five study groups were defined: (a) gellangumwith encapsulated chondrogenic predifferentiated rabbit adipose stem cells (ASCþGF); (b) gellan gum with encapsulated nonchondrogenic predifferentiated rabbit adipose stem cells (ASC); (c) gellan gum with encapsulated rabbit articular chondrocytes (AC) (standard control); (d) gellan gum alone (control); (e) empty defect (control). Fullthickness articular cartilage defects were created and the gellangum constructs were injected and left for 8 weeks. The macroscopic aspect of the explants showed a progressive increase of similarity with the lateral native cartilage, stable integration at the defect site, more pronouncedly in the cell-loaded constructs. Tissue scoring showed that ASCþGF exhibited the best results regarding tissue quality progression. Alcian blue retrieved similar results with a better outcome for the cell-loaded constructs. Regarding real-time PCR analyses, ASCþGF had the best progression with an upregulation of collagen type II and aggrecan, and a downregulation of collagen type I. Gellan gum hydrogels combined with autologous cells constitute a promising approach for the treatment of articular cartilage defects, and adipose derived cellsmayconstitute a valid alternative to currently used articular chondrocytes.application/pdfporInjectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defectsOliveira, J. T.Gardel, Leandro. S.Rada, TommasoMartins, L.Gomes, Manuela E.Reis, R. L.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptPMID20187118ISSNIsPartOf0736-0266DOIIsPartOf10.1002/jor.211142012-10-29T18:32:16Z2010-092010-09-01T00:00:00ZHandlehttps://hdl.handle.net/1822/20590http://purl.org/coar/access_right/c_abf2open accessGellan gumStem cellsCartilageTissue engineeringIn vivo289357 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/c34af55c-e9ad-424e-bfbb-76e7eee34564/download |
| spellingShingle | Injectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defects Oliveira, J. T. Gellan gum Stem cells Cartilage Tissue engineering In vivo |
| status | SINGLETON |
| subject.fl_str_mv | Gellan gum Stem cells Cartilage Tissue engineering In vivo |
| title | Injectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defects |
| title_full | Injectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defects |
| title_fullStr | Injectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defects |
| title_full_unstemmed | Injectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defects |
| title_short | Injectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defects |
| title_sort | Injectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defects |
| topic | Gellan gum Stem cells Cartilage Tissue engineering In vivo |
| topic_facet | Gellan gum Stem cells Cartilage Tissue engineering In vivo |
| url | https://hdl.handle.net/1822/20590 |
| visible | 1 |