Publicação
Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers
| Resumo: | The heterogeneity of the hierarchical tissues requires designing multipart engineered constructs as suitable tissue replacements. Herein, we propose the incorporation of platelet lysate (PL) within electrospun fiber core aiming the fabrication of functionally graded 3D scaffolds for heterotypic tissues regeneration, such as tendon-to-bone interface. First, anisotropic yarns (A-Yarns) and isotropic threads with nanohydroxyapatite (I-Threads/PL@nHAp) are fabricated to recreate the tendon- and bone- microstructures and both incorporated with PL using emulsion electrospinning for a sustained and local delivery of growth factors, cytokines, and chemokines. Biological performance using human adipose-derived stem cells demonstrates that A-Yarns/PL induce a higher expression of scleraxis, a tenogenic-marker, while in I-Threads/PL@nHAp, higher alkaline phosphatase activity and matrix mineralization suggest an osteogenic commitment without the need for external biochemical supplementation when compared to plain controls. As proof of concept, functional 3D gradient scaffolds are fabricated using weaving technique, resulting in 3D textured hierarchical constructs with gradients in composition and topography. Additionally, the precise delivery of bioactive cues together with in situ biophysical features guide the commitment into a phenotypic gradient exhibiting chondrogenic and osteochondrogenic profiles in the interface of scaffolds. Overall, we demonstrate a promising patch solution for the regeneration of tendon-to-bone tissue interface through the fabrication of PL-functional 3D gradient constructs. |
|---|---|
| Autores principais: | Calejo, Isabel |
| Outros Autores: | Reis, R. L.; Domingues, Rui Miguel Andrade; Gomes, Manuela E. |
| Assunto: | Emulsion Electrospinning Functionally graded scaffolds Hierarchical tissues Platelet lysates |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867438231762501632 |
|---|---|
| author | Calejo, Isabel |
| author2 | Reis, R. L. Domingues, Rui Miguel Andrade Gomes, Manuela E. |
| author2_role | author author author |
| author_facet | Calejo, Isabel Reis, R. L. Domingues, Rui Miguel Andrade Gomes, Manuela E. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Calejo, Isabel\"},{\"Person.name\":\"Reis, R. L.\"},{\"Person.name\":\"Domingues, Rui Miguel Andrade\"},{\"Person.name\":\"Gomes, Manuela E.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Calejo, Isabel Reis, R. L. Domingues, Rui Miguel Andrade Gomes, Manuela E. |
| datacite.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-01-01T07:00:25Z |
| datacite.date.embargoed.fl_str_mv | 2023-01-01T07:00:25Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Emulsion Electrospinning Functionally graded scaffolds Hierarchical tissues Platelet lysates |
| datacite.titles.title.fl_str_mv | Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Calejo, Isabel Reis, R. L. Domingues, Rui Miguel Andrade Gomes, Manuela E. |
| dc.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-01-01T07:00:25Z |
| dc.date.embargoed.fl_str_mv | 2023-01-01T07:00:25Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/75587 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Wiley-VCH Verlag |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Emulsion Electrospinning Functionally graded scaffolds Hierarchical tissues Platelet lysates |
| dc.title.fl_str_mv | Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The heterogeneity of the hierarchical tissues requires designing multipart engineered constructs as suitable tissue replacements. Herein, we propose the incorporation of platelet lysate (PL) within electrospun fiber core aiming the fabrication of functionally graded 3D scaffolds for heterotypic tissues regeneration, such as tendon-to-bone interface. First, anisotropic yarns (A-Yarns) and isotropic threads with nanohydroxyapatite (I-Threads/PL@nHAp) are fabricated to recreate the tendon- and bone- microstructures and both incorporated with PL using emulsion electrospinning for a sustained and local delivery of growth factors, cytokines, and chemokines. Biological performance using human adipose-derived stem cells demonstrates that A-Yarns/PL induce a higher expression of scleraxis, a tenogenic-marker, while in I-Threads/PL@nHAp, higher alkaline phosphatase activity and matrix mineralization suggest an osteogenic commitment without the need for external biochemical supplementation when compared to plain controls. As proof of concept, functional 3D gradient scaffolds are fabricated using weaving technique, resulting in 3D textured hierarchical constructs with gradients in composition and topography. Additionally, the precise delivery of bioactive cues together with in situ biophysical features guide the commitment into a phenotypic gradient exhibiting chondrogenic and osteochondrogenic profiles in the interface of scaffolds. Overall, we demonstrate a promising patch solution for the regeneration of tendon-to-bone tissue interface through the fabrication of PL-functional 3D gradient constructs. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/67a6ff9a-1660-41db-bee8-a9afd8562937/download |
| id | rum_694c19d945ebc6877dd477bf18d64323 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/75587 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/75587 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Calejo, Isabel Reis, R. L. Domingues, Rui Miguel Andrade Gomes, Manuela E. |
| publishDate | 2022 |
| publisher.none.fl_str_mv | Wiley-VCH Verlag |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engWiley-VCH VerlagporThe heterogeneity of the hierarchical tissues requires designing multipart engineered constructs as suitable tissue replacements. Herein, we propose the incorporation of platelet lysate (PL) within electrospun fiber core aiming the fabrication of functionally graded 3D scaffolds for heterotypic tissues regeneration, such as tendon-to-bone interface. First, anisotropic yarns (A-Yarns) and isotropic threads with nanohydroxyapatite (I-Threads/PL@nHAp) are fabricated to recreate the tendon- and bone- microstructures and both incorporated with PL using emulsion electrospinning for a sustained and local delivery of growth factors, cytokines, and chemokines. Biological performance using human adipose-derived stem cells demonstrates that A-Yarns/PL induce a higher expression of scleraxis, a tenogenic-marker, while in I-Threads/PL@nHAp, higher alkaline phosphatase activity and matrix mineralization suggest an osteogenic commitment without the need for external biochemical supplementation when compared to plain controls. As proof of concept, functional 3D gradient scaffolds are fabricated using weaving technique, resulting in 3D textured hierarchical constructs with gradients in composition and topography. Additionally, the precise delivery of bioactive cues together with in situ biophysical features guide the commitment into a phenotypic gradient exhibiting chondrogenic and osteochondrogenic profiles in the interface of scaffolds. Overall, we demonstrate a promising patch solution for the regeneration of tendon-to-bone tissue interface through the fabrication of PL-functional 3D gradient constructs.application/pdfporTexturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibersCalejo, IsabelReis, R. L.Domingues, Rui Miguel AndradeGomes, Manuela E.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONCalejo I., Reis R. L., Domingues R. M. A., Gomes M. E. Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers, Advanced Healthcare Materials, doi:10.1002/adhm.202102076, 2021PMID34927396ISSNIsPartOf2192-2640EISSNIsPartOf2192-2659DOIIsPartOf10.1002/adhm.2021020762023-01-01T07:00:25Z20222022-01-19T10:04:27Z2022-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/75587http://purl.org/coar/access_right/c_abf2open accessEmulsion ElectrospinningFunctionally graded scaffoldsHierarchical tissuesPlatelet lysates4025536 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/67a6ff9a-1660-41db-bee8-a9afd8562937/download |
| spellingShingle | Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers Calejo, Isabel Emulsion Electrospinning Functionally graded scaffolds Hierarchical tissues Platelet lysates |
| status | SINGLETON |
| subject.fl_str_mv | Emulsion Electrospinning Functionally graded scaffolds Hierarchical tissues Platelet lysates |
| title | Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers |
| title_full | Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers |
| title_fullStr | Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers |
| title_full_unstemmed | Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers |
| title_short | Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers |
| title_sort | Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers |
| topic | Emulsion Electrospinning Functionally graded scaffolds Hierarchical tissues Platelet lysates |
| topic_facet | Emulsion Electrospinning Functionally graded scaffolds Hierarchical tissues Platelet lysates |
| url | https://hdl.handle.net/1822/75587 |
| visible | 1 |