Publicação
Enhanced oil spill bioremediation with Corksorb
| Resumo: | Regranulated cork particles are by-products of cork stopper production, which present very high hydrophobicity and oil sorption capacity after thermal treatment [1]. These thermally treated granules have been used as absorbents in the remediation of oil spills under the commercial brand Corksorb (Corticeira Amorim, S.G.P.S.). Once saturated with oil, cork should be regenerated for cyclic reuse. For that, hydrocarbonoclastic bacteria can be applied to degrade the oil components. Here, we hypothesize that Corksorb granules can potentially stimulate the activity of these bacteria, due to their unique chemical composition, structure and properties [2], thus improving in situ bioremediation of oil spills. To test this hypothesis, bacterial growth and hydrocarbons biodegradation were assessed in pure cultures of Alcanivorax borkumensis SK2 or Rhodoccocus opacus B4 incubated with a mixture of alkanes, and compared with incubations in which the alkanes were sorbed in corksorb. Growth of Alcanivorax borkumensis SK2 in alkanes was 1.5 times higher in the assays with corksorb, relatively to the assays without corksorb. Moreover, 72% of the added alkanes were biodegraded in the presence of corksorb, while in its absence only 47% were removed. For Rhodococcus opacus B4, hydrocarbons consumption reached 96% and 88% in the presence and absence of corksorb, respectively, although no significant effect could be detected on growth. These results show that corksorb stimulates the activity of hydrocarbonoclastic bacteria, therefore presenting a high potential for improving in situ bioremediation of hydrocarbon-contaminated environments by combining absorption with stimulated biodegradation. The mechanisms underlying this stimulatory effect are currently under study. |
|---|---|
| Autores principais: | Martins, Valdo Ricardo Alves |
| Outros Autores: | Freitas, Carlos J. B.; Carvalho, Ana Rita Castro; Alves, M. M.; Pereira, M. A.; Cavaleiro, Ana Júlia Viana |
| Assunto: | Corksorb Alkanes Bioremediation Alcanivorax borkumensis SK2 Rhodococcus opacus B4 |
| Ano: | 2019 |
| País: | Portugal |
| Tipo de documento: | outro |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866877665330331648 |
|---|---|
| author | Martins, Valdo Ricardo Alves |
| author2 | Freitas, Carlos J. B. Carvalho, Ana Rita Castro Alves, M. M. Pereira, M. A. Cavaleiro, Ana Júlia Viana |
| author2_role | author author author author author |
| author_facet | Martins, Valdo Ricardo Alves Freitas, Carlos J. B. Carvalho, Ana Rita Castro Alves, M. M. Pereira, M. A. Cavaleiro, Ana Júlia Viana |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Martins, Valdo Ricardo Alves\"},{\"Person.name\":\"Freitas, Carlos J. B.\"},{\"Person.name\":\"Carvalho, Ana Rita Castro\"},{\"Person.name\":\"Alves, M. M.\"},{\"Person.name\":\"Pereira, M. A.\"},{\"Person.name\":\"Cavaleiro, Ana Júlia Viana\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Martins, Valdo Ricardo Alves Freitas, Carlos J. B. Carvalho, Ana Rita Castro Alves, M. M. Pereira, M. A. Cavaleiro, Ana Júlia Viana |
| datacite.date.Accepted.fl_str_mv | 2019-10-24T00:00:00Z |
| datacite.date.available.fl_str_mv | 2019-11-04T13:47:20Z |
| datacite.date.embargoed.fl_str_mv | 2019-11-04T13:47:20Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Corksorb Alkanes Bioremediation Alcanivorax borkumensis SK2 Rhodococcus opacus B4 |
| datacite.titles.title.fl_str_mv | Enhanced oil spill bioremediation with Corksorb |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Martins, Valdo Ricardo Alves Freitas, Carlos J. B. Carvalho, Ana Rita Castro Alves, M. M. Pereira, M. A. Cavaleiro, Ana Júlia Viana |
| dc.date.Accepted.fl_str_mv | 2019-10-24T00:00:00Z |
| dc.date.available.fl_str_mv | 2019-11-04T13:47:20Z |
| dc.date.embargoed.fl_str_mv | 2019-11-04T13:47:20Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/61929 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Corksorb Alkanes Bioremediation Alcanivorax borkumensis SK2 Rhodococcus opacus B4 |
| dc.title.fl_str_mv | Enhanced oil spill bioremediation with Corksorb |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_1843 |
| description | Regranulated cork particles are by-products of cork stopper production, which present very high hydrophobicity and oil sorption capacity after thermal treatment [1]. These thermally treated granules have been used as absorbents in the remediation of oil spills under the commercial brand Corksorb (Corticeira Amorim, S.G.P.S.). Once saturated with oil, cork should be regenerated for cyclic reuse. For that, hydrocarbonoclastic bacteria can be applied to degrade the oil components. Here, we hypothesize that Corksorb granules can potentially stimulate the activity of these bacteria, due to their unique chemical composition, structure and properties [2], thus improving in situ bioremediation of oil spills. To test this hypothesis, bacterial growth and hydrocarbons biodegradation were assessed in pure cultures of Alcanivorax borkumensis SK2 or Rhodoccocus opacus B4 incubated with a mixture of alkanes, and compared with incubations in which the alkanes were sorbed in corksorb. Growth of Alcanivorax borkumensis SK2 in alkanes was 1.5 times higher in the assays with corksorb, relatively to the assays without corksorb. Moreover, 72% of the added alkanes were biodegraded in the presence of corksorb, while in its absence only 47% were removed. For Rhodococcus opacus B4, hydrocarbons consumption reached 96% and 88% in the presence and absence of corksorb, respectively, although no significant effect could be detected on growth. These results show that corksorb stimulates the activity of hydrocarbonoclastic bacteria, therefore presenting a high potential for improving in situ bioremediation of hydrocarbon-contaminated environments by combining absorption with stimulated biodegradation. The mechanisms underlying this stimulatory effect are currently under study. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | other |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/b1ef9b11-7afc-4546-bca9-eb7ba805faf2/download |
| id | rum_6fcb65bee97ae511c33d760ce7134be5 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/61929 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/61929 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Martins, Valdo Ricardo Alves Freitas, Carlos J. B. Carvalho, Ana Rita Castro Alves, M. M. Pereira, M. A. Cavaleiro, Ana Júlia Viana |
| publishDate | 2019 |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engporRegranulated cork particles are by-products of cork stopper production, which present very high hydrophobicity and oil sorption capacity after thermal treatment [1]. These thermally treated granules have been used as absorbents in the remediation of oil spills under the commercial brand Corksorb (Corticeira Amorim, S.G.P.S.). Once saturated with oil, cork should be regenerated for cyclic reuse. For that, hydrocarbonoclastic bacteria can be applied to degrade the oil components. Here, we hypothesize that Corksorb granules can potentially stimulate the activity of these bacteria, due to their unique chemical composition, structure and properties [2], thus improving in situ bioremediation of oil spills. To test this hypothesis, bacterial growth and hydrocarbons biodegradation were assessed in pure cultures of Alcanivorax borkumensis SK2 or Rhodoccocus opacus B4 incubated with a mixture of alkanes, and compared with incubations in which the alkanes were sorbed in corksorb. Growth of Alcanivorax borkumensis SK2 in alkanes was 1.5 times higher in the assays with corksorb, relatively to the assays without corksorb. Moreover, 72% of the added alkanes were biodegraded in the presence of corksorb, while in its absence only 47% were removed. For Rhodococcus opacus B4, hydrocarbons consumption reached 96% and 88% in the presence and absence of corksorb, respectively, although no significant effect could be detected on growth. These results show that corksorb stimulates the activity of hydrocarbonoclastic bacteria, therefore presenting a high potential for improving in situ bioremediation of hydrocarbon-contaminated environments by combining absorption with stimulated biodegradation. The mechanisms underlying this stimulatory effect are currently under study.application/pdfporEnhanced oil spill bioremediation with CorksorbMartins, Valdo Ricardo AlvesFreitas, Carlos J. B.Carvalho, Ana Rita CastroAlves, M. M.Pereira, M. A.Cavaleiro, Ana Júlia VianaHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.pt2019-11-04T13:47:20Z2019-10-242019-11-02T12:14:48Z2019-10-24T00:00:00ZHandlehttps://hdl.handle.net/1822/61929http://purl.org/coar/access_right/c_abf2open accessCorksorbAlkanesBioremediationAlcanivorax borkumensis SK2Rhodococcus opacus B4871966 bytesother research producthttp://purl.org/coar/resource_type/c_1843otherhttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/b1ef9b11-7afc-4546-bca9-eb7ba805faf2/download |
| spellingShingle | Enhanced oil spill bioremediation with Corksorb Martins, Valdo Ricardo Alves Corksorb Alkanes Bioremediation Alcanivorax borkumensis SK2 Rhodococcus opacus B4 |
| status | SINGLETON |
| subject.fl_str_mv | Corksorb Alkanes Bioremediation Alcanivorax borkumensis SK2 Rhodococcus opacus B4 |
| title | Enhanced oil spill bioremediation with Corksorb |
| title_full | Enhanced oil spill bioremediation with Corksorb |
| title_fullStr | Enhanced oil spill bioremediation with Corksorb |
| title_full_unstemmed | Enhanced oil spill bioremediation with Corksorb |
| title_short | Enhanced oil spill bioremediation with Corksorb |
| title_sort | Enhanced oil spill bioremediation with Corksorb |
| topic | Corksorb Alkanes Bioremediation Alcanivorax borkumensis SK2 Rhodococcus opacus B4 |
| topic_facet | Corksorb Alkanes Bioremediation Alcanivorax borkumensis SK2 Rhodococcus opacus B4 |
| url | https://hdl.handle.net/1822/61929 |
| visible | 1 |