Publicação
Double membrane based on lidocaine-coated polymyxin-alginate nanoparticles for wound healing: in vitro characterization and in vivo tissue repair
| Resumo: | The aim of this study was to develop and characterize a double layer biomembrane for dual drug delivery to be used for the treatment of wounds. The membrane was composed of chitosan, hydroxypropyl methylcellulose and lidocaine chloride (anesthetic drug) in the first layer, and of sodium alginate-polymyxin B sulphate (antibiotic) nanoparticles as the second layer. A product with excellent thickness (0.01-0.02 mm), adequate mechanical properties with respect to elasticity, stiffness, tension, and compatible pH for lesion application has been successfully obtained. The incorporation of the drugs was confirmed analysing the membrane cross-sections by scanning electron microscopy. A strong interaction between the drugs and the functional groups of respective polymers was confirmed by Fourier-Transform Infrared Spectroscopy, thermal analysis and X-ray diffraction. Microbiological assays showed a high antimicrobial activity when polymyxin B was present to act against the Staphylococcus aureus and Pseudomonas aeruginosa strains. Low cytotoxicity observed in a cell viability colorimetric assay and SEM analysis suggest biocompatibility between the developed biomembrane and the cell culture. The in vivo assay allowed visualizing the healing potential by calculating the wound retraction index and by histological analysis. Our results confirm the effectiveness of the developed innovative biomaterial for tissue repair and regeneration in an animal model. |
|---|---|
| Autores principais: | Oliveira, D. M. L. |
| Outros Autores: | Rezende, P. S.; Barbosa, T. C.; Nalone, L. A.; Bani, C.; Tavares, D. S.; Silva, C. F. da; Chaud, M. V.; Padilha, F.; Cano, A.; Albuquerque Júnior, R. L. C. de; Souto, Eliana B.; Severino, P. |
| Assunto: | double membrane polymyxin B sulphate lidocaine hydrochloride solid lipid nanoparticles burst release controlled release |
| Ano: | 2020 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439874111438848 |
|---|---|
| author | Oliveira, D. M. L. |
| author2 | Rezende, P. S. Barbosa, T. C. Nalone, L. A. Bani, C. Tavares, D. S. Silva, C. F. da Chaud, M. V. Padilha, F. Cano, A. Albuquerque Júnior, R. L. C. de Souto, Eliana B. Severino, P. |
| author2_role | author author author author author author author author author author author author |
| author_facet | Oliveira, D. M. L. Rezende, P. S. Barbosa, T. C. Nalone, L. A. Bani, C. Tavares, D. S. Silva, C. F. da Chaud, M. V. Padilha, F. Cano, A. Albuquerque Júnior, R. L. C. de Souto, Eliana B. Severino, P. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Oliveira, D. M. L.\"},{\"Person.name\":\"Rezende, P. S.\"},{\"Person.name\":\"Barbosa, T. C.\"},{\"Person.name\":\"Nalone, L. A.\"},{\"Person.name\":\"Bani, C.\"},{\"Person.name\":\"Tavares, D. S.\"},{\"Person.name\":\"Silva, C. F. da\"},{\"Person.name\":\"Chaud, M. V.\"},{\"Person.name\":\"Padilha, F.\"},{\"Person.name\":\"Cano, A.\"},{\"Person.name\":\"Albuquerque Júnior, R. L. C. de\"},{\"Person.name\":\"Souto, Eliana B.\"},{\"Person.name\":\"Severino, P.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Oliveira, D. M. L. Rezende, P. S. Barbosa, T. C. Nalone, L. A. Bani, C. Tavares, D. S. Silva, C. F. da Chaud, M. V. Padilha, F. Cano, A. Albuquerque Júnior, R. L. C. de Souto, Eliana B. Severino, P. |
| datacite.date.Accepted.fl_str_mv | 2020-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2020-11-02T17:37:10Z |
| datacite.date.embargoed.fl_str_mv | 2020-11-02T17:37:10Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | double membrane polymyxin B sulphate lidocaine hydrochloride solid lipid nanoparticles burst release controlled release |
| datacite.titles.title.fl_str_mv | Double membrane based on lidocaine-coated polymyxin-alginate nanoparticles for wound healing: in vitro characterization and in vivo tissue repair |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Oliveira, D. M. L. Rezende, P. S. Barbosa, T. C. Nalone, L. A. Bani, C. Tavares, D. S. Silva, C. F. da Chaud, M. V. Padilha, F. Cano, A. Albuquerque Júnior, R. L. C. de Souto, Eliana B. Severino, P. |
| dc.date.Accepted.fl_str_mv | 2020-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2020-11-02T17:37:10Z |
| dc.date.embargoed.fl_str_mv | 2020-11-02T17:37:10Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/67979 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | double membrane polymyxin B sulphate lidocaine hydrochloride solid lipid nanoparticles burst release controlled release |
| dc.title.fl_str_mv | Double membrane based on lidocaine-coated polymyxin-alginate nanoparticles for wound healing: in vitro characterization and in vivo tissue repair |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The aim of this study was to develop and characterize a double layer biomembrane for dual drug delivery to be used for the treatment of wounds. The membrane was composed of chitosan, hydroxypropyl methylcellulose and lidocaine chloride (anesthetic drug) in the first layer, and of sodium alginate-polymyxin B sulphate (antibiotic) nanoparticles as the second layer. A product with excellent thickness (0.01-0.02 mm), adequate mechanical properties with respect to elasticity, stiffness, tension, and compatible pH for lesion application has been successfully obtained. The incorporation of the drugs was confirmed analysing the membrane cross-sections by scanning electron microscopy. A strong interaction between the drugs and the functional groups of respective polymers was confirmed by Fourier-Transform Infrared Spectroscopy, thermal analysis and X-ray diffraction. Microbiological assays showed a high antimicrobial activity when polymyxin B was present to act against the Staphylococcus aureus and Pseudomonas aeruginosa strains. Low cytotoxicity observed in a cell viability colorimetric assay and SEM analysis suggest biocompatibility between the developed biomembrane and the cell culture. The in vivo assay allowed visualizing the healing potential by calculating the wound retraction index and by histological analysis. Our results confirm the effectiveness of the developed innovative biomaterial for tissue repair and regeneration in an animal model. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/b9a94d1d-566c-4e19-9d29-794059c01810/download |
| id | rum_711fcb4a3189cd493003fee320e7461c |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/67979 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/67979 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Oliveira, D. M. L. Rezende, P. S. Barbosa, T. C. Nalone, L. A. Bani, C. Tavares, D. S. Silva, C. F. da Chaud, M. V. Padilha, F. Cano, A. Albuquerque Júnior, R. L. C. de Souto, Eliana B. Severino, P. |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engElsevierporThe aim of this study was to develop and characterize a double layer biomembrane for dual drug delivery to be used for the treatment of wounds. The membrane was composed of chitosan, hydroxypropyl methylcellulose and lidocaine chloride (anesthetic drug) in the first layer, and of sodium alginate-polymyxin B sulphate (antibiotic) nanoparticles as the second layer. A product with excellent thickness (0.01-0.02 mm), adequate mechanical properties with respect to elasticity, stiffness, tension, and compatible pH for lesion application has been successfully obtained. The incorporation of the drugs was confirmed analysing the membrane cross-sections by scanning electron microscopy. A strong interaction between the drugs and the functional groups of respective polymers was confirmed by Fourier-Transform Infrared Spectroscopy, thermal analysis and X-ray diffraction. Microbiological assays showed a high antimicrobial activity when polymyxin B was present to act against the Staphylococcus aureus and Pseudomonas aeruginosa strains. Low cytotoxicity observed in a cell viability colorimetric assay and SEM analysis suggest biocompatibility between the developed biomembrane and the cell culture. The in vivo assay allowed visualizing the healing potential by calculating the wound retraction index and by histological analysis. Our results confirm the effectiveness of the developed innovative biomaterial for tissue repair and regeneration in an animal model.application/pdfporDouble membrane based on lidocaine-coated polymyxin-alginate nanoparticles for wound healing: in vitro characterization and in vivo tissue repairOliveira, D. M. L.Rezende, P. S.Barbosa, T. C.Nalone, L. A.Bani, C.Tavares, D. S.Silva, C. F. daChaud, M. V.Padilha, F.Cano, A.Albuquerque Júnior, R. L. C. deSouto, Eliana B.Severino, P.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONOliveira, D. M. L.; Rezende, P. S.; Barbosa, T. C.; Nalone, L. A.; Bani, C.; Tavares, D. S.; Silva, C. F. da; Chaud, M. V.; Padilha, F.; Cano, A.; Albuquerque Júnior, R. L. C. de; Souto, Eliana; Severino, P., Double membrane based on lidocaine-coated polymyxin-alginate nanoparticles for wound healing: in vitro characterization and in vivo tissue repair. International Journal of Pharmaceutics, 591(120001), 2020PMID33141086ISSNIsPartOf0378-5173DOIIsPartOf10.1016/j.ijpharm.2020.1200012020-11-02T17:37:10Z20202020-10-31T11:45:15Z2020-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/67979http://purl.org/coar/access_right/c_abf2open accessdouble membranepolymyxin B sulphatelidocaine hydrochloridesolid lipid nanoparticlesburst releasecontrolled release15587734 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/b9a94d1d-566c-4e19-9d29-794059c01810/download |
| spellingShingle | Double membrane based on lidocaine-coated polymyxin-alginate nanoparticles for wound healing: in vitro characterization and in vivo tissue repair Oliveira, D. M. L. double membrane polymyxin B sulphate lidocaine hydrochloride solid lipid nanoparticles burst release controlled release |
| status | SINGLETON |
| subject.fl_str_mv | double membrane polymyxin B sulphate lidocaine hydrochloride solid lipid nanoparticles burst release controlled release |
| title | Double membrane based on lidocaine-coated polymyxin-alginate nanoparticles for wound healing: in vitro characterization and in vivo tissue repair |
| title_full | Double membrane based on lidocaine-coated polymyxin-alginate nanoparticles for wound healing: in vitro characterization and in vivo tissue repair |
| title_fullStr | Double membrane based on lidocaine-coated polymyxin-alginate nanoparticles for wound healing: in vitro characterization and in vivo tissue repair |
| title_full_unstemmed | Double membrane based on lidocaine-coated polymyxin-alginate nanoparticles for wound healing: in vitro characterization and in vivo tissue repair |
| title_short | Double membrane based on lidocaine-coated polymyxin-alginate nanoparticles for wound healing: in vitro characterization and in vivo tissue repair |
| title_sort | Double membrane based on lidocaine-coated polymyxin-alginate nanoparticles for wound healing: in vitro characterization and in vivo tissue repair |
| topic | double membrane polymyxin B sulphate lidocaine hydrochloride solid lipid nanoparticles burst release controlled release |
| topic_facet | double membrane polymyxin B sulphate lidocaine hydrochloride solid lipid nanoparticles burst release controlled release |
| url | https://hdl.handle.net/1822/67979 |
| visible | 1 |