Publicação
Interleukin-8 and Interleukin-6 are biomarkers of poor prognosis in Esophageal squamous cell carcinoma
| Resumo: | Esophageal squamous cell carcinoma (ESCC) is a common type of cancer characterized by fast progression and high mortality rates, which generally implies a poor prognosis at time of diagnosis. Intricate interaction networks of cytokines produced by resident and inflammatory cells in the tumor microenvironment play crucial roles in ESCC development and metastasis, thus influencing therapy efficiency. As such, cytokines are the most prominent targets for specific therapies and prognostic parameters to predict tumor progression and aggressiveness. In this work, we examined the association between ESCC progression and the systemic levels of inflammatory cytokines to determine their usefulness as diagnostic biomarkers. We analyzed the levels of IL-1β, IL-6, IL-8, IL-10, TNF-α e IL-12p70 in a group of 70 ESCC patients and 70 healthy individuals using Cytometric Bead Array (CBA) technology. We detected increased levels of IL-1β, IL-6, IL-8, and IL-10 in ESCC patients compared to controls. However, multivariate analysis revealed that only IL8 was an independent prognostic factor for ESCC, as were the well-known risk factors: alcohol consumption, tobacco usage, and exposure to pesticides/insecticides. Importantly, patients with low IL-6, IL-8, TNM I/II, or those who underwent surgery had a significantly higher overall survival rate. We also studied cultured Kyse-30 and Kyse-410 cells in mice. We determined that the ESCC cell line Kyse-30 grew more aggressively than the Kyse-410 cell line. This enhanced growth was associated with the recruitment/accumulation of intratumoral polymorphonuclear leukocytes. In conclusion, our data suggest IL-8 as a valuable prognostic factor with potential as a biomarker for ESCC. |
|---|---|
| Autores principais: | Pastrez, Paula Roberta Aguiar |
| Outros Autores: | Barbosa, Ana Margarida; Mariano, Vânia Sammartino; Causin, Rhafaela Lima; Castro, António G.; Torrado, Egídio; Longatto, Adhemar |
| Assunto: | Cytokines Tumor microenvironment Sophageal cancer Inflammation Interleukin-8 Interleukin-6 esophageal cancer |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866876209161306112 |
|---|---|
| author | Pastrez, Paula Roberta Aguiar |
| author2 | Barbosa, Ana Margarida Mariano, Vânia Sammartino Causin, Rhafaela Lima Castro, António G. Torrado, Egídio Longatto, Adhemar |
| author2_role | author author author author author author |
| author_facet | Pastrez, Paula Roberta Aguiar Barbosa, Ana Margarida Mariano, Vânia Sammartino Causin, Rhafaela Lima Castro, António G. Torrado, Egídio Longatto, Adhemar |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Pastrez, Paula Roberta Aguiar\"},{\"Person.name\":\"Barbosa, Ana Margarida\"},{\"Person.name\":\"Mariano, Vânia Sammartino\"},{\"Person.name\":\"Causin, Rhafaela Lima\"},{\"Person.name\":\"Castro, António G.\"},{\"Person.name\":\"Torrado, Egídio\"},{\"Person.name\":\"Longatto, Adhemar\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Pastrez, Paula Roberta Aguiar Barbosa, Ana Margarida Mariano, Vânia Sammartino Causin, Rhafaela Lima Castro, António G. Torrado, Egídio Longatto, Adhemar |
| datacite.date.Accepted.fl_str_mv | 2023-03-27T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-07-06T17:25:24Z |
| datacite.date.embargoed.fl_str_mv | 2023-07-06T17:25:24Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Cytokines Tumor microenvironment Sophageal cancer Inflammation Interleukin-8 Interleukin-6 esophageal cancer |
| datacite.titles.title.fl_str_mv | Interleukin-8 and Interleukin-6 are biomarkers of poor prognosis in Esophageal squamous cell carcinoma |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Pastrez, Paula Roberta Aguiar Barbosa, Ana Margarida Mariano, Vânia Sammartino Causin, Rhafaela Lima Castro, António G. Torrado, Egídio Longatto, Adhemar |
| dc.date.Accepted.fl_str_mv | 2023-03-27T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-07-06T17:25:24Z |
| dc.date.embargoed.fl_str_mv | 2023-07-06T17:25:24Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/85426 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Multidisciplinary Digital Publishing Institute |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.rights.rights.copyright.fl_str_mv | openAccess |
| dc.subject.none.fl_str_mv | Cytokines Tumor microenvironment Sophageal cancer Inflammation Interleukin-8 Interleukin-6 esophageal cancer |
| dc.title.fl_str_mv | Interleukin-8 and Interleukin-6 are biomarkers of poor prognosis in Esophageal squamous cell carcinoma |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Esophageal squamous cell carcinoma (ESCC) is a common type of cancer characterized by fast progression and high mortality rates, which generally implies a poor prognosis at time of diagnosis. Intricate interaction networks of cytokines produced by resident and inflammatory cells in the tumor microenvironment play crucial roles in ESCC development and metastasis, thus influencing therapy efficiency. As such, cytokines are the most prominent targets for specific therapies and prognostic parameters to predict tumor progression and aggressiveness. In this work, we examined the association between ESCC progression and the systemic levels of inflammatory cytokines to determine their usefulness as diagnostic biomarkers. We analyzed the levels of IL-1β, IL-6, IL-8, IL-10, TNF-α e IL-12p70 in a group of 70 ESCC patients and 70 healthy individuals using Cytometric Bead Array (CBA) technology. We detected increased levels of IL-1β, IL-6, IL-8, and IL-10 in ESCC patients compared to controls. However, multivariate analysis revealed that only IL8 was an independent prognostic factor for ESCC, as were the well-known risk factors: alcohol consumption, tobacco usage, and exposure to pesticides/insecticides. Importantly, patients with low IL-6, IL-8, TNM I/II, or those who underwent surgery had a significantly higher overall survival rate. We also studied cultured Kyse-30 and Kyse-410 cells in mice. We determined that the ESCC cell line Kyse-30 grew more aggressively than the Kyse-410 cell line. This enhanced growth was associated with the recruitment/accumulation of intratumoral polymorphonuclear leukocytes. In conclusion, our data suggest IL-8 as a valuable prognostic factor with potential as a biomarker for ESCC. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/8ab87a6d-84ab-4e3b-a1aa-76b06694d733/download |
| id | rum_72eabe434d04caeea669c6cfa1a40b66 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/85426 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/85426 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Pastrez, Paula Roberta Aguiar Barbosa, Ana Margarida Mariano, Vânia Sammartino Causin, Rhafaela Lima Castro, António G. Torrado, Egídio Longatto, Adhemar |
| publishDate | 2023 |
| publisher.none.fl_str_mv | Multidisciplinary Digital Publishing Institute |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engMultidisciplinary Digital Publishing InstituteporEsophageal squamous cell carcinoma (ESCC) is a common type of cancer characterized by fast progression and high mortality rates, which generally implies a poor prognosis at time of diagnosis. Intricate interaction networks of cytokines produced by resident and inflammatory cells in the tumor microenvironment play crucial roles in ESCC development and metastasis, thus influencing therapy efficiency. As such, cytokines are the most prominent targets for specific therapies and prognostic parameters to predict tumor progression and aggressiveness. In this work, we examined the association between ESCC progression and the systemic levels of inflammatory cytokines to determine their usefulness as diagnostic biomarkers. We analyzed the levels of IL-1β, IL-6, IL-8, IL-10, TNF-α e IL-12p70 in a group of 70 ESCC patients and 70 healthy individuals using Cytometric Bead Array (CBA) technology. We detected increased levels of IL-1β, IL-6, IL-8, and IL-10 in ESCC patients compared to controls. However, multivariate analysis revealed that only IL8 was an independent prognostic factor for ESCC, as were the well-known risk factors: alcohol consumption, tobacco usage, and exposure to pesticides/insecticides. Importantly, patients with low IL-6, IL-8, TNM I/II, or those who underwent surgery had a significantly higher overall survival rate. We also studied cultured Kyse-30 and Kyse-410 cells in mice. We determined that the ESCC cell line Kyse-30 grew more aggressively than the Kyse-410 cell line. This enhanced growth was associated with the recruitment/accumulation of intratumoral polymorphonuclear leukocytes. In conclusion, our data suggest IL-8 as a valuable prognostic factor with potential as a biomarker for ESCC.application/pdfporInterleukin-8 and Interleukin-6 are biomarkers of poor prognosis in Esophageal squamous cell carcinomaPastrez, Paula Roberta AguiarBarbosa, Ana MargaridaMariano, Vânia SammartinoCausin, Rhafaela LimaCastro, António G.Torrado, EgídioLongatto, AdhemarHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptDOIIsPartOf10.3390/cancers150719972023-07-06T17:25:24Z2023-03-272023-04-12T13:24:32Z2023-03-27T00:00:00ZHandlehttps://hdl.handle.net/1822/85426http://purl.org/coar/access_right/c_abf2open accessCytokinesTumor microenvironmentSophageal cancerInflammationInterleukin-8Interleukin-6esophageal cancer2500479 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2023-03-27http://creativecommons.org/licenses/by/4.0/openAccesshttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/8ab87a6d-84ab-4e3b-a1aa-76b06694d733/download |
| spellingShingle | Interleukin-8 and Interleukin-6 are biomarkers of poor prognosis in Esophageal squamous cell carcinoma Pastrez, Paula Roberta Aguiar Cytokines Tumor microenvironment Sophageal cancer Inflammation Interleukin-8 Interleukin-6 esophageal cancer |
| status | SINGLETON |
| subject.fl_str_mv | Cytokines Tumor microenvironment Sophageal cancer Inflammation Interleukin-8 Interleukin-6 esophageal cancer |
| title | Interleukin-8 and Interleukin-6 are biomarkers of poor prognosis in Esophageal squamous cell carcinoma |
| title_full | Interleukin-8 and Interleukin-6 are biomarkers of poor prognosis in Esophageal squamous cell carcinoma |
| title_fullStr | Interleukin-8 and Interleukin-6 are biomarkers of poor prognosis in Esophageal squamous cell carcinoma |
| title_full_unstemmed | Interleukin-8 and Interleukin-6 are biomarkers of poor prognosis in Esophageal squamous cell carcinoma |
| title_short | Interleukin-8 and Interleukin-6 are biomarkers of poor prognosis in Esophageal squamous cell carcinoma |
| title_sort | Interleukin-8 and Interleukin-6 are biomarkers of poor prognosis in Esophageal squamous cell carcinoma |
| topic | Cytokines Tumor microenvironment Sophageal cancer Inflammation Interleukin-8 Interleukin-6 esophageal cancer |
| topic_facet | Cytokines Tumor microenvironment Sophageal cancer Inflammation Interleukin-8 Interleukin-6 esophageal cancer |
| url | https://hdl.handle.net/1822/85426 |
| visible | 1 |