Publicação
Pronociceptive changes in the activity of rostroventromedial medulla (RVM) pain modulatory cells in the streptozotocin-diabetic rat
| Resumo: | Neuropathic pain is one of the most frequent complications of diabetes. The increased neuronal activity of primary afferents and spinal cord neurons in streptozotocin (STZ)-diabetic rats increases the recruitment of the nociceptive ascending pathways, which may affect the activity of pain control circuits in the brain. This study aimed to characterize the electrophysiological responses of neurons of the rostroventromedial medulla (RVM), a key brainstem area involved in descending modulation of nociceptive neurotransmission at the spinal cord, in STZ-diabetic rats. Spontaneous and noxious-evoked activity of ON-like cells (pain facilitatory cells) and OFF-like cells (pain inhibitory cells) in the RVM were analyzed by single cell extracellular electrophysiological recordings in STZ-diabetic rats with behavioral signs of diabetic neuropathic pain 4 weeks after diabetes induction and in age-matched non-diabetic controls (CTRL). The electrophysiological analysis revealed an increase in the spontaneous activity of RVM pronociceptive ON-like cells in STZ-diabetic rats when compared to CTRL On the contrary, the number of active antinociceptive OFF-like cells was significantly lower in the STZ-diabetic rats and their spontaneous activity was decreased when compared with CTRL Overall, the changes in the activity of RVM pain modulatory cells in STZ-diabetic rats point to enhancement of descending pain facilitation. Based on similar results obtained at the RVM in traumatic neuropathic pain models, the changes in the electrophysiological responses of RVM in STZ-diabetic rats may account for exacerbated pain-like behaviors in diabetic neuropathy. |
|---|---|
| Autores principais: | Silva, M. |
| Outros Autores: | Amorim, Diana; Almeida, Armando; Tavares, I.; Ribeiro, Filipa Pinto; Morgado, C. |
| Assunto: | Diabetic neuropathic pain RVM Descending pain modulation Nociception STZ-diabetic rat Neuronal activity Ciências Médicas::Medicina Clínica |
| Ano: | 2013 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867438359660462080 |
|---|---|
| author | Silva, M. |
| author2 | Amorim, Diana Almeida, Armando Tavares, I. Ribeiro, Filipa Pinto Morgado, C. |
| author2_role | author author author author author |
| author_facet | Silva, M. Amorim, Diana Almeida, Armando Tavares, I. Ribeiro, Filipa Pinto Morgado, C. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Silva, M.\"},{\"Person.name\":\"Amorim, Diana\"},{\"Person.name\":\"Almeida, Armando\"},{\"Person.name\":\"Tavares, I.\"},{\"Person.name\":\"Ribeiro, Filipa Pinto\"},{\"Person.name\":\"Morgado, C.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Silva, M. Amorim, Diana Almeida, Armando Tavares, I. Ribeiro, Filipa Pinto Morgado, C. |
| datacite.date.Accepted.fl_str_mv | 2013-07-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2015-09-16T16:54:50Z |
| datacite.date.embargoed.fl_str_mv | 2015-09-16T16:54:50Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Diabetic neuropathic pain RVM Descending pain modulation Nociception STZ-diabetic rat Neuronal activity Ciências Médicas::Medicina Clínica |
| datacite.titles.title.fl_str_mv | Pronociceptive changes in the activity of rostroventromedial medulla (RVM) pain modulatory cells in the streptozotocin-diabetic rat |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Silva, M. Amorim, Diana Almeida, Armando Tavares, I. Ribeiro, Filipa Pinto Morgado, C. |
| dc.date.Accepted.fl_str_mv | 2013-07-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2015-09-16T16:54:50Z |
| dc.date.embargoed.fl_str_mv | 2015-09-16T16:54:50Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/37260 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Diabetic neuropathic pain RVM Descending pain modulation Nociception STZ-diabetic rat Neuronal activity Ciências Médicas::Medicina Clínica |
| dc.title.fl_str_mv | Pronociceptive changes in the activity of rostroventromedial medulla (RVM) pain modulatory cells in the streptozotocin-diabetic rat |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Neuropathic pain is one of the most frequent complications of diabetes. The increased neuronal activity of primary afferents and spinal cord neurons in streptozotocin (STZ)-diabetic rats increases the recruitment of the nociceptive ascending pathways, which may affect the activity of pain control circuits in the brain. This study aimed to characterize the electrophysiological responses of neurons of the rostroventromedial medulla (RVM), a key brainstem area involved in descending modulation of nociceptive neurotransmission at the spinal cord, in STZ-diabetic rats. Spontaneous and noxious-evoked activity of ON-like cells (pain facilitatory cells) and OFF-like cells (pain inhibitory cells) in the RVM were analyzed by single cell extracellular electrophysiological recordings in STZ-diabetic rats with behavioral signs of diabetic neuropathic pain 4 weeks after diabetes induction and in age-matched non-diabetic controls (CTRL). The electrophysiological analysis revealed an increase in the spontaneous activity of RVM pronociceptive ON-like cells in STZ-diabetic rats when compared to CTRL On the contrary, the number of active antinociceptive OFF-like cells was significantly lower in the STZ-diabetic rats and their spontaneous activity was decreased when compared with CTRL Overall, the changes in the activity of RVM pain modulatory cells in STZ-diabetic rats point to enhancement of descending pain facilitation. Based on similar results obtained at the RVM in traumatic neuropathic pain models, the changes in the electrophysiological responses of RVM in STZ-diabetic rats may account for exacerbated pain-like behaviors in diabetic neuropathy. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/54528eb2-6406-489e-9f98-9025ce5a5bf3/download |
| id | rum_779f4d01067ef6145e5ccf69a4e161ec |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/37260 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/37260 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Silva, M. Amorim, Diana Almeida, Armando Tavares, I. Ribeiro, Filipa Pinto Morgado, C. |
| publishDate | 2013 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engElsevierporNeuropathic pain is one of the most frequent complications of diabetes. The increased neuronal activity of primary afferents and spinal cord neurons in streptozotocin (STZ)-diabetic rats increases the recruitment of the nociceptive ascending pathways, which may affect the activity of pain control circuits in the brain. This study aimed to characterize the electrophysiological responses of neurons of the rostroventromedial medulla (RVM), a key brainstem area involved in descending modulation of nociceptive neurotransmission at the spinal cord, in STZ-diabetic rats. Spontaneous and noxious-evoked activity of ON-like cells (pain facilitatory cells) and OFF-like cells (pain inhibitory cells) in the RVM were analyzed by single cell extracellular electrophysiological recordings in STZ-diabetic rats with behavioral signs of diabetic neuropathic pain 4 weeks after diabetes induction and in age-matched non-diabetic controls (CTRL). The electrophysiological analysis revealed an increase in the spontaneous activity of RVM pronociceptive ON-like cells in STZ-diabetic rats when compared to CTRL On the contrary, the number of active antinociceptive OFF-like cells was significantly lower in the STZ-diabetic rats and their spontaneous activity was decreased when compared with CTRL Overall, the changes in the activity of RVM pain modulatory cells in STZ-diabetic rats point to enhancement of descending pain facilitation. Based on similar results obtained at the RVM in traumatic neuropathic pain models, the changes in the electrophysiological responses of RVM in STZ-diabetic rats may account for exacerbated pain-like behaviors in diabetic neuropathy.application/pdfporPronociceptive changes in the activity of rostroventromedial medulla (RVM) pain modulatory cells in the streptozotocin-diabetic ratSilva, M.Amorim, DianaAlmeida, ArmandoTavares, I.Ribeiro, Filipa PintoMorgado, C.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONSilva, M., Amorim, D., Almeida, A., Tavares, I., Pinto-Ribeiro, F., & Morgado, C. (2013). Pronociceptive changes in the activity of rostroventromedial medulla (RVM) pain modulatory cells in the streptozotocin-diabetic rat. Brain research bulletin, 96, 39-44PMID23644033ISSNIsPartOf0361-9230DOIIsPartOf10.1016/j.brainresbull.2013.04.0082015-09-16T16:54:50Z2013-072012-122015-01-30T16:12:26Z2013-07-01T00:00:00ZHandlehttps://hdl.handle.net/1822/37260http://purl.org/coar/access_right/c_16ecrestricted accessDiabetic neuropathic painRVMDescending pain modulationNociceptionSTZ-diabetic ratNeuronal activityhttp://www.oecd.org/science/inno/38235147.pdfFields of Science and Technology (FOS)Ciências Médicas::Medicina Clínica1029416 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorium.uminho.pt/bitstreams/54528eb2-6406-489e-9f98-9025ce5a5bf3/download |
| spellingShingle | Pronociceptive changes in the activity of rostroventromedial medulla (RVM) pain modulatory cells in the streptozotocin-diabetic rat Silva, M. Diabetic neuropathic pain RVM Descending pain modulation Nociception STZ-diabetic rat Neuronal activity Ciências Médicas::Medicina Clínica |
| status | SINGLETON |
| subject.fl_str_mv | Diabetic neuropathic pain RVM Descending pain modulation Nociception STZ-diabetic rat Neuronal activity |
| subject.other.fl_str_mv | Ciências Médicas::Medicina Clínica |
| title | Pronociceptive changes in the activity of rostroventromedial medulla (RVM) pain modulatory cells in the streptozotocin-diabetic rat |
| title_full | Pronociceptive changes in the activity of rostroventromedial medulla (RVM) pain modulatory cells in the streptozotocin-diabetic rat |
| title_fullStr | Pronociceptive changes in the activity of rostroventromedial medulla (RVM) pain modulatory cells in the streptozotocin-diabetic rat |
| title_full_unstemmed | Pronociceptive changes in the activity of rostroventromedial medulla (RVM) pain modulatory cells in the streptozotocin-diabetic rat |
| title_short | Pronociceptive changes in the activity of rostroventromedial medulla (RVM) pain modulatory cells in the streptozotocin-diabetic rat |
| title_sort | Pronociceptive changes in the activity of rostroventromedial medulla (RVM) pain modulatory cells in the streptozotocin-diabetic rat |
| topic | Diabetic neuropathic pain RVM Descending pain modulation Nociception STZ-diabetic rat Neuronal activity Ciências Médicas::Medicina Clínica |
| topic_facet | Diabetic neuropathic pain RVM Descending pain modulation Nociception STZ-diabetic rat Neuronal activity Ciências Médicas::Medicina Clínica |
| url | https://hdl.handle.net/1822/37260 |
| visible | 1 |