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Protein-engineered polymers functionalized with antimicrobial peptides for the development of active surfaces

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Resumo:Antibacterial resistance is a major worldwide threat due to the increasing number of infections caused by antibiotic-resistant bacteria with medical devices being a major source of these infections. This suggests the need for new antimicrobial biomaterial designs able to withstand the increasing pressure of antimicrobial resistance. Recombinant protein polymers (rPPs) are an emerging class of nature-inspired biopolymers with unique chemical, physical and biological properties. These polymers can be functionalized with antimicrobial molecules utilizing recombinant DNA technology and then produced in microbial cell factories. In this work, we report the functionalization of rPBPs based on elastin and silk-elastin with different antimicrobial peptides (AMPs). These polymers were produced in <i>Escherichia coli</i>, successfully purified by employing non-chromatographic processes, and used for the production of free-standing films. The antimicrobial activity of the materials was evaluated against Gram-positive and Gram-negative bacteria, and results showed that the polymers demonstrated antimicrobial activity, pointing out the potential of these biopolymers for the development of new advanced antimicrobial materials.
Autores principais:Pereira, Ana Margarida Macedo Bernardes
Outros Autores:Gomes, Diana Cristina Serra; da Costa, André; Dias, Simoni Campos; Casal, Margarida; Machado, Raul
Assunto:Antimicrobial resistance Antimicrobial surfaces Antimicrobial films Recombinant protein polymers Silk-elastin proteins Elastin-like recombinamers Antimicrobial peptides
Ano:2021
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho

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