Publicação
Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L. an ecophysiological, ultrastructural and molecular approach
| Resumo: | This study aimed to assess the toxicity of glyphosate (GLY; 0, 10, 20 and 30?mg?kg-1) in Solanum lycopersicum L., particularly focusing on the photosynthetic metabolism. By combining ecophysiological, ultrastructural, biochemical and molecular tools, the results revealed that the exposure of tomato plants to GLY led to alterations in leaf water balance regulation [increasing stomatal conductance (gs) and decreasing water use efficiency (WUEi) at higher concentrations] and induced slight alterations in the structural integrity of cells, mainly in chloroplasts, accompanied by a loss of cell viability. Moreover, the transcriptional and biochemical control of several photosynthetic-related parameters was reduced upon GLY exposure. However, in vivo chlorophyll fluorometry and IRGA gas-exchange studies revealed that the photosynthetic yield of S. lycopersicum was not repressed by GLY. Overall, GLY impacts cellular and subcellular homeostasis (by affecting chloroplast structure, reducing photosynthetic pigments and inhibiting photosynthetic-related genes transcription), and leaf structure, but is not reducing the carbon flow on a leaf area basis. Altogether, these results suggest a trade-off effect in which GLY-induced toxicity is compensated by a higher photosynthetic activity related to GLY-induced dysfunction in gs and an increase in mesophyll thickness/density, allowing the viable leaf cells to maintain their photosynthetic capacity. |
|---|---|
| Autores principais: | Soares, Cristiano |
| Outros Autores: | Pereira, Ruth; Martins, Maria; Tamagnini, Paula; Serôdio, João; Moutinho-Pereira, José; Cunha, Ana; Fidalgo, Fernanda |
| Assunto: | Non-target plants Abiotic stress Photochemistry Calvin cycle Chlorophyll fluorometry gas-exchange |
| Ano: | 2020 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867437872209985536 |
|---|---|
| author | Soares, Cristiano |
| author2 | Pereira, Ruth Martins, Maria Tamagnini, Paula Serôdio, João Moutinho-Pereira, José Cunha, Ana Fidalgo, Fernanda |
| author2_role | author author author author author author author |
| author_facet | Soares, Cristiano Pereira, Ruth Martins, Maria Tamagnini, Paula Serôdio, João Moutinho-Pereira, José Cunha, Ana Fidalgo, Fernanda |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Soares, Cristiano\"},{\"Person.name\":\"Pereira, Ruth\"},{\"Person.name\":\"Martins, Maria\"},{\"Person.name\":\"Tamagnini, Paula\"},{\"Person.name\":\"Serôdio, João\"},{\"Person.name\":\"Moutinho-Pereira, José\"},{\"Person.name\":\"Cunha, Ana\"},{\"Person.name\":\"Fidalgo, Fernanda\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Soares, Cristiano Pereira, Ruth Martins, Maria Tamagnini, Paula Serôdio, João Moutinho-Pereira, José Cunha, Ana Fidalgo, Fernanda |
| datacite.date.Accepted.fl_str_mv | 2020-11-05T00:00:00Z |
| datacite.date.available.fl_str_mv | 2020-05-26T09:09:08Z |
| datacite.date.embargoed.fl_str_mv | 2020-05-26T09:09:08Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Non-target plants Abiotic stress Photochemistry Calvin cycle Chlorophyll fluorometry gas-exchange |
| datacite.titles.title.fl_str_mv | Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L. an ecophysiological, ultrastructural and molecular approach |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Soares, Cristiano Pereira, Ruth Martins, Maria Tamagnini, Paula Serôdio, João Moutinho-Pereira, José Cunha, Ana Fidalgo, Fernanda |
| dc.date.Accepted.fl_str_mv | 2020-11-05T00:00:00Z |
| dc.date.available.fl_str_mv | 2020-05-26T09:09:08Z |
| dc.date.embargoed.fl_str_mv | 2020-05-26T09:09:08Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/65480 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Non-target plants Abiotic stress Photochemistry Calvin cycle Chlorophyll fluorometry gas-exchange |
| dc.title.fl_str_mv | Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L. an ecophysiological, ultrastructural and molecular approach |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | This study aimed to assess the toxicity of glyphosate (GLY; 0, 10, 20 and 30?mg?kg-1) in Solanum lycopersicum L., particularly focusing on the photosynthetic metabolism. By combining ecophysiological, ultrastructural, biochemical and molecular tools, the results revealed that the exposure of tomato plants to GLY led to alterations in leaf water balance regulation [increasing stomatal conductance (gs) and decreasing water use efficiency (WUEi) at higher concentrations] and induced slight alterations in the structural integrity of cells, mainly in chloroplasts, accompanied by a loss of cell viability. Moreover, the transcriptional and biochemical control of several photosynthetic-related parameters was reduced upon GLY exposure. However, in vivo chlorophyll fluorometry and IRGA gas-exchange studies revealed that the photosynthetic yield of S. lycopersicum was not repressed by GLY. Overall, GLY impacts cellular and subcellular homeostasis (by affecting chloroplast structure, reducing photosynthetic pigments and inhibiting photosynthetic-related genes transcription), and leaf structure, but is not reducing the carbon flow on a leaf area basis. Altogether, these results suggest a trade-off effect in which GLY-induced toxicity is compensated by a higher photosynthetic activity related to GLY-induced dysfunction in gs and an increase in mesophyll thickness/density, allowing the viable leaf cells to maintain their photosynthetic capacity. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/c725206d-58c8-43ab-af4b-8f84d7722477/download |
| id | rum_7a8febf714d13d5154a9bd84e0da8a49 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/65480 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/65480 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Soares, Cristiano Pereira, Ruth Martins, Maria Tamagnini, Paula Serôdio, João Moutinho-Pereira, José Cunha, Ana Fidalgo, Fernanda |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engElsevierporThis study aimed to assess the toxicity of glyphosate (GLY; 0, 10, 20 and 30?mg?kg-1) in Solanum lycopersicum L., particularly focusing on the photosynthetic metabolism. By combining ecophysiological, ultrastructural, biochemical and molecular tools, the results revealed that the exposure of tomato plants to GLY led to alterations in leaf water balance regulation [increasing stomatal conductance (gs) and decreasing water use efficiency (WUEi) at higher concentrations] and induced slight alterations in the structural integrity of cells, mainly in chloroplasts, accompanied by a loss of cell viability. Moreover, the transcriptional and biochemical control of several photosynthetic-related parameters was reduced upon GLY exposure. However, in vivo chlorophyll fluorometry and IRGA gas-exchange studies revealed that the photosynthetic yield of S. lycopersicum was not repressed by GLY. Overall, GLY impacts cellular and subcellular homeostasis (by affecting chloroplast structure, reducing photosynthetic pigments and inhibiting photosynthetic-related genes transcription), and leaf structure, but is not reducing the carbon flow on a leaf area basis. Altogether, these results suggest a trade-off effect in which GLY-induced toxicity is compensated by a higher photosynthetic activity related to GLY-induced dysfunction in gs and an increase in mesophyll thickness/density, allowing the viable leaf cells to maintain their photosynthetic capacity.application/pdfporGlyphosate-dependent effects on photosynthesis of Solanum lycopersicum L. an ecophysiological, ultrastructural and molecular approachSoares, CristianoPereira, RuthMartins, MariaTamagnini, PaulaSerôdio, JoãoMoutinho-Pereira, JoséCunha, AnaFidalgo, FernandaHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONSoares, Cristiano; Pereira, Ruth; Martins, Maria; Tamagnini, Paula; Serôdio, João; Moutinho-Pereira, José; Cunha, Ana; Fidalgo, Fernanda, Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L. an ecophysiological, ultrastructural and molecular approach. Journal of Hazardous Materials, 398(122871), 2020PMID32450466ISSNIsPartOf0304-3894DOIIsPartOf10.1016/j.jhazmat.2020.1228712020-05-26T09:09:08Z2020-11-052020-05-23T13:53:02Z2020-11-05T00:00:00ZHandlehttps://hdl.handle.net/1822/65480http://purl.org/coar/access_right/c_abf2open accessNon-target plantsAbiotic stressPhotochemistryCalvin cycleChlorophyll fluorometrygas-exchange6547976 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/c725206d-58c8-43ab-af4b-8f84d7722477/download |
| spellingShingle | Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L. an ecophysiological, ultrastructural and molecular approach Soares, Cristiano Non-target plants Abiotic stress Photochemistry Calvin cycle Chlorophyll fluorometry gas-exchange |
| status | SINGLETON |
| subject.fl_str_mv | Non-target plants Abiotic stress Photochemistry Calvin cycle Chlorophyll fluorometry gas-exchange |
| title | Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L. an ecophysiological, ultrastructural and molecular approach |
| title_full | Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L. an ecophysiological, ultrastructural and molecular approach |
| title_fullStr | Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L. an ecophysiological, ultrastructural and molecular approach |
| title_full_unstemmed | Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L. an ecophysiological, ultrastructural and molecular approach |
| title_short | Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L. an ecophysiological, ultrastructural and molecular approach |
| title_sort | Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L. an ecophysiological, ultrastructural and molecular approach |
| topic | Non-target plants Abiotic stress Photochemistry Calvin cycle Chlorophyll fluorometry gas-exchange |
| topic_facet | Non-target plants Abiotic stress Photochemistry Calvin cycle Chlorophyll fluorometry gas-exchange |
| url | https://hdl.handle.net/1822/65480 |
| visible | 1 |