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An efficient software tool to segment slice and view electron tomograms

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Resumo:Segmentation is a key method to extract useful information in Electron Tomography. Manual segmentation is the most commonly used method, but it is subject to user bias and the process is slow. The lack of adequate automated processes, due to the high complexity and to the low signal-to-noise ratio of these tomograms, provided the main challenges for this dissertation: to develop a software tool to efficiently handle electron tomograms, including a novel 3D segmentation algorithm. Tomograms can be seen as a stack of 2D images; operations on tomograms usually lead to computationally intense tasks. This is due to the large amount of involved data and to the strided and random memory access patterns. These characteristics represent serious problems on novel computing systems, which rely on complex memory hierarchy architectures to hide memory access latency time. A software tool with a user-friendly interface — TomSeg — was designed, implemented and tested with experimental datasets, built with sequences of Scanning Electron Microscopy images obtained using a Slice and View technique. This tool lets users align, crop, segment and export electron tomograms, using computationally efficient processes. TomSeg takes advantage of the most usual architectures of modern compute servers, namely based on multicore and many-core CPU devices, exploring vector and parallel programming techniques; it also explores the available GPU-devices to speedup critical code functions. Validation and performance results on a compute server are presented together with the performance improvements obtained during the implementation and test phases. TomSeg is an open-source tool for Unix and Windows that can be easily extended with new algorithms to efficiently handle generic tomograms.
Autores principais:Sousa, Paulo Rafael da Costa e
Ano:2017
País:Portugal
Tipo de documento:dissertação de mestrado
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho
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author Sousa, Paulo Rafael da Costa e
author_facet Sousa, Paulo Rafael da Costa e
author_role author
contributor_name_str_mv Proença, Alberto José
Stroppa, Daniel Grando
Universidade do Minho
country_str PT
creators_json_txt [{\"Person.name\":\"Sousa, Paulo Rafael da Costa e\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Proença, Alberto José
Stroppa, Daniel Grando
Universidade do Minho
datacite.creators.creator.creatorName.fl_str_mv Sousa, Paulo Rafael da Costa e
datacite.date.Accepted.fl_str_mv 2017-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2019-04-11T16:01:44Z
datacite.date.embargoed.fl_str_mv 2019-04-11T16:01:44Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.titles.title.fl_str_mv An efficient software tool to segment slice and view electron tomograms
dc.contributor.none.fl_str_mv Proença, Alberto José
Stroppa, Daniel Grando
Universidade do Minho
dc.creator.none.fl_str_mv Sousa, Paulo Rafael da Costa e
dc.date.Accepted.fl_str_mv 2017-01-01T00:00:00Z
dc.date.available.fl_str_mv 2019-04-11T16:01:44Z
dc.date.embargoed.fl_str_mv 2019-04-11T16:01:44Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv https://hdl.handle.net/1822/60003
dc.language.none.fl_str_mv eng
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.title.fl_str_mv An efficient software tool to segment slice and view electron tomograms
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_bdcc
description Segmentation is a key method to extract useful information in Electron Tomography. Manual segmentation is the most commonly used method, but it is subject to user bias and the process is slow. The lack of adequate automated processes, due to the high complexity and to the low signal-to-noise ratio of these tomograms, provided the main challenges for this dissertation: to develop a software tool to efficiently handle electron tomograms, including a novel 3D segmentation algorithm. Tomograms can be seen as a stack of 2D images; operations on tomograms usually lead to computationally intense tasks. This is due to the large amount of involved data and to the strided and random memory access patterns. These characteristics represent serious problems on novel computing systems, which rely on complex memory hierarchy architectures to hide memory access latency time. A software tool with a user-friendly interface — TomSeg — was designed, implemented and tested with experimental datasets, built with sequences of Scanning Electron Microscopy images obtained using a Slice and View technique. This tool lets users align, crop, segment and export electron tomograms, using computationally efficient processes. TomSeg takes advantage of the most usual architectures of modern compute servers, namely based on multicore and many-core CPU devices, exploring vector and parallel programming techniques; it also explores the available GPU-devices to speedup critical code functions. Validation and performance results on a compute server are presented together with the performance improvements obtained during the implementation and test phases. TomSeg is an open-source tool for Unix and Windows that can be easily extended with new algorithms to efficiently handle generic tomograms.
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spelling engporSegmentation is a key method to extract useful information in Electron Tomography. Manual segmentation is the most commonly used method, but it is subject to user bias and the process is slow. The lack of adequate automated processes, due to the high complexity and to the low signal-to-noise ratio of these tomograms, provided the main challenges for this dissertation: to develop a software tool to efficiently handle electron tomograms, including a novel 3D segmentation algorithm. Tomograms can be seen as a stack of 2D images; operations on tomograms usually lead to computationally intense tasks. This is due to the large amount of involved data and to the strided and random memory access patterns. These characteristics represent serious problems on novel computing systems, which rely on complex memory hierarchy architectures to hide memory access latency time. A software tool with a user-friendly interface — TomSeg — was designed, implemented and tested with experimental datasets, built with sequences of Scanning Electron Microscopy images obtained using a Slice and View technique. This tool lets users align, crop, segment and export electron tomograms, using computationally efficient processes. TomSeg takes advantage of the most usual architectures of modern compute servers, namely based on multicore and many-core CPU devices, exploring vector and parallel programming techniques; it also explores the available GPU-devices to speedup critical code functions. Validation and performance results on a compute server are presented together with the performance improvements obtained during the implementation and test phases. TomSeg is an open-source tool for Unix and Windows that can be easily extended with new algorithms to efficiently handle generic tomograms.application/pdfporAn efficient software tool to segment slice and view electron tomogramsSousa, Paulo Rafael da Costa eProença, Alberto JoséStroppa, Daniel GrandoHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptURNurn:tid:2022026402019-04-11T16:01:44Z201720172017-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/60003http://purl.org/coar/access_right/c_abf2open access30191502 bytesliteraturehttp://purl.org/coar/resource_type/c_bdccmaster thesishttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/9628faab-516a-4e31-bb80-d07e1772bcac/download
spellingShingle An efficient software tool to segment slice and view electron tomograms
Sousa, Paulo Rafael da Costa e
status SINGLETON
title An efficient software tool to segment slice and view electron tomograms
title_full An efficient software tool to segment slice and view electron tomograms
title_fullStr An efficient software tool to segment slice and view electron tomograms
title_full_unstemmed An efficient software tool to segment slice and view electron tomograms
title_short An efficient software tool to segment slice and view electron tomograms
title_sort An efficient software tool to segment slice and view electron tomograms
url https://hdl.handle.net/1822/60003
visible 1