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A comparison between bright field and phase-contrast image analysis techniques in activated sludge morphological characterization

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Resumo:Different approaches using microscopy image analysis procedures were employed for characterization of activated sludge systems. The approaches varied mainly on the type of visualization and acquisition method used for collection of data. In this context, this study focused on the comparison of the two most common acquisition methods: bright field and phase-contrast microscopy. Images were acquired from seven different wastewater treatment plants for a combined period of two years. Advantages and disadvantages of each acquisition technique and the results are discussed. Bright field microscopy proved to be more simple and inexpensive and provided the best overall results.
Autores principais:Mesquita, D. P.
Outros Autores:Dias, Oscar; Amaral, A. L.; Ferreira, Eugénio C.
Assunto:Activated sludge Image analysis Bright field Phase contrast Aggregates Filaments
Ano:2010
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho
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author Mesquita, D. P.
author2 Dias, Oscar
Amaral, A. L.
Ferreira, Eugénio C.
author2_role author
author
author
author_facet Mesquita, D. P.
Dias, Oscar
Amaral, A. L.
Ferreira, Eugénio C.
author_role author
contributor_name_str_mv Universidade do Minho
country_str PT
creators_json_txt [{\"Person.name\":\"Mesquita, D. P.\"},{\"Person.name\":\"Dias, Oscar\"},{\"Person.name\":\"Amaral, A. L.\"},{\"Person.name\":\"Ferreira, Eugénio C.\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Universidade do Minho
datacite.creators.creator.creatorName.fl_str_mv Mesquita, D. P.
Dias, Oscar
Amaral, A. L.
Ferreira, Eugénio C.
datacite.date.Accepted.fl_str_mv 2010-04-01T00:00:00Z
datacite.date.available.fl_str_mv 2010-04-14T11:46:01Z
datacite.date.embargoed.fl_str_mv 2010-04-14T11:46:01Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv Activated sludge
Image analysis
Bright field
Phase contrast
Aggregates
Filaments
datacite.titles.title.fl_str_mv A comparison between bright field and phase-contrast image analysis techniques in activated sludge morphological characterization
dc.contributor.none.fl_str_mv Universidade do Minho
dc.creator.none.fl_str_mv Mesquita, D. P.
Dias, Oscar
Amaral, A. L.
Ferreira, Eugénio C.
dc.date.Accepted.fl_str_mv 2010-04-01T00:00:00Z
dc.date.available.fl_str_mv 2010-04-14T11:46:01Z
dc.date.embargoed.fl_str_mv 2010-04-14T11:46:01Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv https://hdl.handle.net/1822/10530
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv Microscopy Society of America
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv Activated sludge
Image analysis
Bright field
Phase contrast
Aggregates
Filaments
dc.title.fl_str_mv A comparison between bright field and phase-contrast image analysis techniques in activated sludge morphological characterization
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description Different approaches using microscopy image analysis procedures were employed for characterization of activated sludge systems. The approaches varied mainly on the type of visualization and acquisition method used for collection of data. In this context, this study focused on the comparison of the two most common acquisition methods: bright field and phase-contrast microscopy. Images were acquired from seven different wastewater treatment plants for a combined period of two years. Advantages and disadvantages of each acquisition technique and the results are discussed. Bright field microscopy proved to be more simple and inexpensive and provided the best overall results.
dirty 0
eu_rights_str_mv openAccess
format article
fulltext.url.fl_str_mv https://prod-dspace.uminho.pt/bitstreams/9fd2917f-48c7-4ff1-a576-29512c59824e/download
id rum_7ef09c19f7259768bc9c4d8a47d420f9
identifier.url.fl_str_mv https://hdl.handle.net/1822/10530
instacron_str repositorium
institution Universidade do Minho
instname_str Universidade do Minho
language eng
network_acronym_str rum
network_name_str RepositóriUM - Universidade do Minho
oai_identifier_str oai:repositorium.uminho.pt:1822/10530
organization_str_mv urn:organizationAcronym:repositorium
person_str_mv Mesquita, D. P.
Dias, Oscar
Amaral, A. L.
Ferreira, Eugénio C.
publishDate 2010
publisher.none.fl_str_mv Microscopy Society of America
reponame_str RepositóriUM - Universidade do Minho
repository_id_str urn:repositoryAcronym:rum
service_str_mv urn:repositoryAcronym:rum
spelling engMicroscopy Society of AmericaporDifferent approaches using microscopy image analysis procedures were employed for characterization of activated sludge systems. The approaches varied mainly on the type of visualization and acquisition method used for collection of data. In this context, this study focused on the comparison of the two most common acquisition methods: bright field and phase-contrast microscopy. Images were acquired from seven different wastewater treatment plants for a combined period of two years. Advantages and disadvantages of each acquisition technique and the results are discussed. Bright field microscopy proved to be more simple and inexpensive and provided the best overall results.application/pdfporA comparison between bright field and phase-contrast image analysis techniques in activated sludge morphological characterizationMesquita, D. P.Dias, OscarAmaral, A. L.Ferreira, Eugénio C.HostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf1431-9276ISSNIsPartOf1435-8115DOIIsPartOf10.1017/S14319276099913582010-04-14T11:46:01Z2010-042010-04-01T00:00:00ZHandlehttps://hdl.handle.net/1822/10530http://purl.org/coar/access_right/c_abf2open accessActivated sludgeImage analysisBright fieldPhase contrastAggregatesFilaments337707 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/9fd2917f-48c7-4ff1-a576-29512c59824e/download
spellingShingle A comparison between bright field and phase-contrast image analysis techniques in activated sludge morphological characterization
Mesquita, D. P.
Activated sludge
Image analysis
Bright field
Phase contrast
Aggregates
Filaments
status SINGLETON
subject.fl_str_mv Activated sludge
Image analysis
Bright field
Phase contrast
Aggregates
Filaments
title A comparison between bright field and phase-contrast image analysis techniques in activated sludge morphological characterization
title_full A comparison between bright field and phase-contrast image analysis techniques in activated sludge morphological characterization
title_fullStr A comparison between bright field and phase-contrast image analysis techniques in activated sludge morphological characterization
title_full_unstemmed A comparison between bright field and phase-contrast image analysis techniques in activated sludge morphological characterization
title_short A comparison between bright field and phase-contrast image analysis techniques in activated sludge morphological characterization
title_sort A comparison between bright field and phase-contrast image analysis techniques in activated sludge morphological characterization
topic Activated sludge
Image analysis
Bright field
Phase contrast
Aggregates
Filaments
topic_facet Activated sludge
Image analysis
Bright field
Phase contrast
Aggregates
Filaments
url https://hdl.handle.net/1822/10530
visible 1