Publicação
The characterization and cytotoxic evaluation of Chondrosia reniformis collagen isolated from different body parts (ectosome and choanosome) envisaging the development of biomaterials
| Resumo: | Chondrosia reniformis is a collagen-rich marine sponge that is considered a sustainable and viable option for producing an alternative to mammalian-origin collagens. However, there is a lack of knowledge regarding the properties of collagen isolated from different sponge parts, namely the outer region, or cortex, (ectosome) and the inner region (choanosome), and how it affects the development of biomaterials. In this study, a brief histological analysis focusing on C. reniformis collagen spatial distribution and a comprehensive comparative analysis between collagen isolated from ectosome and choanosome are presented. The isolated collagen characterization was based on isolation yield, Fourier-transformed infrared spectroscopy (FTIR), circular dichroism (CD), SDS-PAGE, dot blot, and amino acid composition, as well as their cytocompatibility envisaging the development of future biomedical applications. An isolation yield of approximately 20% was similar for both sponge parts, as well as the FTIR, CD, and SDS-PAGE profiles, which demonstrated that both isolated collagens presented a high purity degree and preserved their triple helix and fibrillar conformation. Ectosome collagen had a higher OHpro content and possessed collagen type I and IV, while the choanosome was predominately constituted by collagen type IV. In vitro cytotoxicity assays using the L929 fibroblast cell line displayed a significant cytotoxic effect of choanosome collagen at 2 mg/mL, while ectosome collagen enhanced cell metabolism and proliferation, thus indicating the latter as being more suitable for the development of biomaterials. This research represents a unique comparative study of C. reniformis body parts, serving as a support for further establishing this marine sponge as a promising alternative collagen source for the future development of biomedical applications. |
|---|---|
| Autores principais: | Rocha, Miguel Soares |
| Outros Autores: | Marques, Ana Catarina Fernandes; Carvalho, Ana Cristina Pontes; Martins, Eva; Ereskovsky, Alexander; Reis, R. L.; Silva, Tiago H. |
| Assunto: | Collagen C. reniformis Marine sponges Ectosome Choanosome |
| Ano: | 2024 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866271281944461312 |
|---|---|
| author | Rocha, Miguel Soares |
| author2 | Marques, Ana Catarina Fernandes Carvalho, Ana Cristina Pontes Martins, Eva Ereskovsky, Alexander Reis, R. L. Silva, Tiago H. |
| author2_role | author author author author author author |
| author_facet | Rocha, Miguel Soares Marques, Ana Catarina Fernandes Carvalho, Ana Cristina Pontes Martins, Eva Ereskovsky, Alexander Reis, R. L. Silva, Tiago H. |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Rocha, Miguel Soares\"},{\"Person.name\":\"Marques, Ana Catarina Fernandes\"},{\"Person.name\":\"Carvalho, Ana Cristina Pontes\"},{\"Person.name\":\"Martins, Eva\"},{\"Person.name\":\"Ereskovsky, Alexander\"},{\"Person.name\":\"Reis, R. L.\"},{\"Person.name\":\"Silva, Tiago H.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Rocha, Miguel Soares Marques, Ana Catarina Fernandes Carvalho, Ana Cristina Pontes Martins, Eva Ereskovsky, Alexander Reis, R. L. Silva, Tiago H. |
| datacite.date.Accepted.fl_str_mv | 2024-01-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Collagen C. reniformis Marine sponges Ectosome Choanosome |
| datacite.titles.title.fl_str_mv | The characterization and cytotoxic evaluation of Chondrosia reniformis collagen isolated from different body parts (ectosome and choanosome) envisaging the development of biomaterials |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Rocha, Miguel Soares Marques, Ana Catarina Fernandes Carvalho, Ana Cristina Pontes Martins, Eva Ereskovsky, Alexander Reis, R. L. Silva, Tiago H. |
| dc.date.Accepted.fl_str_mv | 2024-01-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/97819 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Multidisciplinary Digital Publishing Institute (MDPI) |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.rights.rights.copyright.fl_str_mv | openAccess |
| dc.subject.none.fl_str_mv | Collagen C. reniformis Marine sponges Ectosome Choanosome |
| dc.title.fl_str_mv | The characterization and cytotoxic evaluation of Chondrosia reniformis collagen isolated from different body parts (ectosome and choanosome) envisaging the development of biomaterials |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Chondrosia reniformis is a collagen-rich marine sponge that is considered a sustainable and viable option for producing an alternative to mammalian-origin collagens. However, there is a lack of knowledge regarding the properties of collagen isolated from different sponge parts, namely the outer region, or cortex, (ectosome) and the inner region (choanosome), and how it affects the development of biomaterials. In this study, a brief histological analysis focusing on C. reniformis collagen spatial distribution and a comprehensive comparative analysis between collagen isolated from ectosome and choanosome are presented. The isolated collagen characterization was based on isolation yield, Fourier-transformed infrared spectroscopy (FTIR), circular dichroism (CD), SDS-PAGE, dot blot, and amino acid composition, as well as their cytocompatibility envisaging the development of future biomedical applications. An isolation yield of approximately 20% was similar for both sponge parts, as well as the FTIR, CD, and SDS-PAGE profiles, which demonstrated that both isolated collagens presented a high purity degree and preserved their triple helix and fibrillar conformation. Ectosome collagen had a higher OHpro content and possessed collagen type I and IV, while the choanosome was predominately constituted by collagen type IV. In vitro cytotoxicity assays using the L929 fibroblast cell line displayed a significant cytotoxic effect of choanosome collagen at 2 mg/mL, while ectosome collagen enhanced cell metabolism and proliferation, thus indicating the latter as being more suitable for the development of biomaterials. This research represents a unique comparative study of C. reniformis body parts, serving as a support for further establishing this marine sponge as a promising alternative collagen source for the future development of biomedical applications. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/f1f25bc9-a391-4a95-a41c-b1720d1b62ff/download |
| funding.funder.alternateName_str_mv | other other other |
| funding.funder.identifier_str_mv | urn:openaire:fct_________::FCT urn:openaire:fct_________::FCT urn:openaire:fct_________::FCT |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia, I.P. Fundação para a Ciência e a Tecnologia, I.P. Fundação para a Ciência e a Tecnologia, I.P. |
| funding.identifier_str_mv | PD/BD/143091/2018 CEECIND/04687/2017/CP1458/CT0030 PINFRA/22190/2016 |
| funding.name_str_mv | CEEC IND 2017 Concurso para Financiamento de Projetos de Infraestruturas de Investigação inseridas no Roteiro Nacional de Infraestruturas de Investigação de Interesse Estratégico |
| funding_str_mv | PD/BD/143091/2018 https://hdl.handle.net/1822/97814 CEECIND/04687/2017/CP1458/CT0030 https://hdl.handle.net/1822/97816 PINFRA/22190/2016 https://hdl.handle.net/1822/97818 |
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| identifier.url.fl_str_mv | https://hdl.handle.net/1822/97819 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/97819 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Rocha, Miguel Soares Marques, Ana Catarina Fernandes Carvalho, Ana Cristina Pontes Martins, Eva Ereskovsky, Alexander Reis, R. L. Silva, Tiago H. |
| publishDate | 2024 |
| publisher.none.fl_str_mv | Multidisciplinary Digital Publishing Institute (MDPI) |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engMultidisciplinary Digital Publishing Institute (MDPI)engChondrosia reniformis is a collagen-rich marine sponge that is considered a sustainable and viable option for producing an alternative to mammalian-origin collagens. However, there is a lack of knowledge regarding the properties of collagen isolated from different sponge parts, namely the outer region, or cortex, (ectosome) and the inner region (choanosome), and how it affects the development of biomaterials. In this study, a brief histological analysis focusing on C. reniformis collagen spatial distribution and a comprehensive comparative analysis between collagen isolated from ectosome and choanosome are presented. The isolated collagen characterization was based on isolation yield, Fourier-transformed infrared spectroscopy (FTIR), circular dichroism (CD), SDS-PAGE, dot blot, and amino acid composition, as well as their cytocompatibility envisaging the development of future biomedical applications. An isolation yield of approximately 20% was similar for both sponge parts, as well as the FTIR, CD, and SDS-PAGE profiles, which demonstrated that both isolated collagens presented a high purity degree and preserved their triple helix and fibrillar conformation. Ectosome collagen had a higher OHpro content and possessed collagen type I and IV, while the choanosome was predominately constituted by collagen type IV. In vitro cytotoxicity assays using the L929 fibroblast cell line displayed a significant cytotoxic effect of choanosome collagen at 2 mg/mL, while ectosome collagen enhanced cell metabolism and proliferation, thus indicating the latter as being more suitable for the development of biomaterials. This research represents a unique comparative study of C. reniformis body parts, serving as a support for further establishing this marine sponge as a promising alternative collagen source for the future development of biomedical applications.application/pdfengThe characterization and cytotoxic evaluation of Chondrosia reniformis collagen isolated from different body parts (ectosome and choanosome) envisaging the development of biomaterialsRocha, Miguel SoaresMarques, Ana Catarina FernandesCarvalho, Ana Cristina PontesMartins, EvaEreskovsky, AlexanderReis, R. L.Silva, Tiago H.HostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptDOIIsPartOf10.3390/md2202005520242025-062025-08-11T15:45:19Z2024-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/97819http://purl.org/coar/access_right/c_abf2open accessCollagenC. reniformisMarine spongesEctosomeChoanosome9365914 bytesFundação para a Ciência e a Tecnologia, I.P.Extraction and purification of collagens and aggregating factors from marine sponges and echinoderms for the production of biomimetic hydrogels (PD/BD/143091/2018)https://hdl.handle.net/1822/97814PD/BD/143091/2018Crossref Funder IDurn:openaire:fct_________::FCTFundação para a Ciência e a Tecnologia, I.P.Marine collagen and ionic-doped calcium phosphates biogenic inks for bone tissue engineering (CEECIND/04687/2017/CP1458/CT0030)CEEC IND 2017https://hdl.handle.net/1822/97816CEECIND/04687/2017/CP1458/CT0030Crossref Funder IDurn:openaire:fct_________::FCTFundação para a Ciência e a Tecnologia, I.P.Tissue Engineering and Regenerative Medicine Infrastructure (PINFRA/22190/2016)Concurso para Financiamento de Projetos de Infraestruturas de Investigação inseridas no Roteiro Nacional de Infraestruturas de Investigação de Interesse Estratégicohttps://hdl.handle.net/1822/97818PINFRA/22190/2016Crossref Funder IDurn:openaire:fct_________::FCTliteraturehttp://purl.org/coar/resource_type/c_6501journal article2024http://creativecommons.org/licenses/by/4.0/openAccesshttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/f1f25bc9-a391-4a95-a41c-b1720d1b62ff/download222119 |
| spellingShingle | The characterization and cytotoxic evaluation of Chondrosia reniformis collagen isolated from different body parts (ectosome and choanosome) envisaging the development of biomaterials Rocha, Miguel Soares Collagen C. reniformis Marine sponges Ectosome Choanosome |
| status | SINGLETON |
| subject.fl_str_mv | Collagen C. reniformis Marine sponges Ectosome Choanosome |
| title | The characterization and cytotoxic evaluation of Chondrosia reniformis collagen isolated from different body parts (ectosome and choanosome) envisaging the development of biomaterials |
| title_full | The characterization and cytotoxic evaluation of Chondrosia reniformis collagen isolated from different body parts (ectosome and choanosome) envisaging the development of biomaterials |
| title_fullStr | The characterization and cytotoxic evaluation of Chondrosia reniformis collagen isolated from different body parts (ectosome and choanosome) envisaging the development of biomaterials |
| title_full_unstemmed | The characterization and cytotoxic evaluation of Chondrosia reniformis collagen isolated from different body parts (ectosome and choanosome) envisaging the development of biomaterials |
| title_short | The characterization and cytotoxic evaluation of Chondrosia reniformis collagen isolated from different body parts (ectosome and choanosome) envisaging the development of biomaterials |
| title_sort | The characterization and cytotoxic evaluation of Chondrosia reniformis collagen isolated from different body parts (ectosome and choanosome) envisaging the development of biomaterials |
| topic | Collagen C. reniformis Marine sponges Ectosome Choanosome |
| topic_facet | Collagen C. reniformis Marine sponges Ectosome Choanosome |
| url | https://hdl.handle.net/1822/97819 |
| visible | 1 |
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