Publicação
Fluorescence studies of the interaction of pyrenylmethyl tributylphosphonium bromide with double-strand polynucleotides
| Resumo: | The interaction between pyren-1-ylmethyl tri-n-butylphosphonium bromide (PMTP), a water-soluble cationic pyrene derivative and the double-strand polynucleotides Poly[dA-dT], Poly[dA].Poly[dT], Poly[dG-dC] and Poly[dG]. Poly[dC] was studied using UV-Vis absorption and fluorescence spectroscopy. The PMTP probe interacts with polynucleotides through both weak and intercalative binding, evidenced through changes in the absorption spectrum (hypochromicity and red shift). The two binding types were distinguished using time-resolved fluorescence, as the intercalative environment differs from that of the surface. Thymine and cytosine are more efficient quenchers of PMTP situated on the surface because of the higher proton accessibility to this region. In contrast, adenine does not quench PMTP fluorescence, whereas guanine residues are always very efficient quenching sites. Therefore, through the use of spectroscopic techniques, it was possible to obtain information concerning the partition of PMTP in each form of binding. In the heteropolymers, Poly[dA-dT] and Poly[dG-dC], PMTP exhibits a significant preference for intercalation in AT sequences, while with GC, the intercalation is lower. In the homopolymers, Poly[dA].Poly[dT] and Poly[dG].Poly[dC], the main mechanism of interaction is weak binding, but some base preferences are elucidated. Additionally, Poly[dA].Poly[dT] can be distinguished from Poly[dA-dT] through a direct energy-transfer process between several bound PMTP molecules. |
|---|---|
| Autores principais: | Oliveira, M. Elisabete |
| Outros Autores: | Baptista, Adelina L. F.; Coutinho, Paulo J. G.; Castanheira, Elisabete M. S.; Hungerford, Graham |
| Assunto: | Polynucleotides Fluorescence Pyrene derivatives |
| Ano: | 2004 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867438272411598848 |
|---|---|
| author | Oliveira, M. Elisabete |
| author2 | Baptista, Adelina L. F. Coutinho, Paulo J. G. Castanheira, Elisabete M. S. Hungerford, Graham |
| author2_role | author author author author |
| author_facet | Oliveira, M. Elisabete Baptista, Adelina L. F. Coutinho, Paulo J. G. Castanheira, Elisabete M. S. Hungerford, Graham |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Oliveira, M. Elisabete\"},{\"Person.name\":\"Baptista, Adelina L. F.\"},{\"Person.name\":\"Coutinho, Paulo J. G.\"},{\"Person.name\":\"Castanheira, Elisabete M. S.\"},{\"Person.name\":\"Hungerford, Graham\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Oliveira, M. Elisabete Baptista, Adelina L. F. Coutinho, Paulo J. G. Castanheira, Elisabete M. S. Hungerford, Graham |
| datacite.date.Accepted.fl_str_mv | 2004-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2006-06-21T14:16:35Z |
| datacite.date.embargoed.fl_str_mv | 2006-06-21T14:16:35Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Polynucleotides Fluorescence Pyrene derivatives |
| datacite.titles.title.fl_str_mv | Fluorescence studies of the interaction of pyrenylmethyl tributylphosphonium bromide with double-strand polynucleotides |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Oliveira, M. Elisabete Baptista, Adelina L. F. Coutinho, Paulo J. G. Castanheira, Elisabete M. S. Hungerford, Graham |
| dc.date.Accepted.fl_str_mv | 2004-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2006-06-21T14:16:35Z |
| dc.date.embargoed.fl_str_mv | 2006-06-21T14:16:35Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/5141 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Royal Society of Chemistry |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Polynucleotides Fluorescence Pyrene derivatives |
| dc.title.fl_str_mv | Fluorescence studies of the interaction of pyrenylmethyl tributylphosphonium bromide with double-strand polynucleotides |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The interaction between pyren-1-ylmethyl tri-n-butylphosphonium bromide (PMTP), a water-soluble cationic pyrene derivative and the double-strand polynucleotides Poly[dA-dT], Poly[dA].Poly[dT], Poly[dG-dC] and Poly[dG]. Poly[dC] was studied using UV-Vis absorption and fluorescence spectroscopy. The PMTP probe interacts with polynucleotides through both weak and intercalative binding, evidenced through changes in the absorption spectrum (hypochromicity and red shift). The two binding types were distinguished using time-resolved fluorescence, as the intercalative environment differs from that of the surface. Thymine and cytosine are more efficient quenchers of PMTP situated on the surface because of the higher proton accessibility to this region. In contrast, adenine does not quench PMTP fluorescence, whereas guanine residues are always very efficient quenching sites. Therefore, through the use of spectroscopic techniques, it was possible to obtain information concerning the partition of PMTP in each form of binding. In the heteropolymers, Poly[dA-dT] and Poly[dG-dC], PMTP exhibits a significant preference for intercalation in AT sequences, while with GC, the intercalation is lower. In the homopolymers, Poly[dA].Poly[dT] and Poly[dG].Poly[dC], the main mechanism of interaction is weak binding, but some base preferences are elucidated. Additionally, Poly[dA].Poly[dT] can be distinguished from Poly[dA-dT] through a direct energy-transfer process between several bound PMTP molecules. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/213b4046-4681-4f40-aba4-db207671f35d/download |
| id | rum_7fa1efd9da77958b8f5f2d3d93236ef7 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/5141 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/5141 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Oliveira, M. Elisabete Baptista, Adelina L. F. Coutinho, Paulo J. G. Castanheira, Elisabete M. S. Hungerford, Graham |
| publishDate | 2004 |
| publisher.none.fl_str_mv | Royal Society of Chemistry |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engRoyal Society of ChemistryengThe interaction between pyren-1-ylmethyl tri-n-butylphosphonium bromide (PMTP), a water-soluble cationic pyrene derivative and the double-strand polynucleotides Poly[dA-dT], Poly[dA].Poly[dT], Poly[dG-dC] and Poly[dG]. Poly[dC] was studied using UV-Vis absorption and fluorescence spectroscopy. The PMTP probe interacts with polynucleotides through both weak and intercalative binding, evidenced through changes in the absorption spectrum (hypochromicity and red shift). The two binding types were distinguished using time-resolved fluorescence, as the intercalative environment differs from that of the surface. Thymine and cytosine are more efficient quenchers of PMTP situated on the surface because of the higher proton accessibility to this region. In contrast, adenine does not quench PMTP fluorescence, whereas guanine residues are always very efficient quenching sites. Therefore, through the use of spectroscopic techniques, it was possible to obtain information concerning the partition of PMTP in each form of binding. In the heteropolymers, Poly[dA-dT] and Poly[dG-dC], PMTP exhibits a significant preference for intercalation in AT sequences, while with GC, the intercalation is lower. In the homopolymers, Poly[dA].Poly[dT] and Poly[dG].Poly[dC], the main mechanism of interaction is weak binding, but some base preferences are elucidated. Additionally, Poly[dA].Poly[dT] can be distinguished from Poly[dA-dT] through a direct energy-transfer process between several bound PMTP molecules.application/pdfengFluorescence studies of the interaction of pyrenylmethyl tributylphosphonium bromide with double-strand polynucleotidesOliveira, M. ElisabeteBaptista, Adelina L. F.Coutinho, Paulo J. G.Castanheira, Elisabete M. S.Hungerford, GrahamHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATION"Photochemical & Photobiological Sciences". ISSN 1474-905X. 3:2 (2004) 217-225.PMID14872240ISSNIsPartOf1474-905XDOIIsPartOf10.1039/b305225g2006-06-21T14:16:35Z20042004-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/5141http://purl.org/coar/access_right/c_abf2open accessPolynucleotidesFluorescencePyrene derivatives210058 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/213b4046-4681-4f40-aba4-db207671f35d/download |
| spellingShingle | Fluorescence studies of the interaction of pyrenylmethyl tributylphosphonium bromide with double-strand polynucleotides Oliveira, M. Elisabete Polynucleotides Fluorescence Pyrene derivatives |
| status | SINGLETON |
| subject.fl_str_mv | Polynucleotides Fluorescence Pyrene derivatives |
| title | Fluorescence studies of the interaction of pyrenylmethyl tributylphosphonium bromide with double-strand polynucleotides |
| title_full | Fluorescence studies of the interaction of pyrenylmethyl tributylphosphonium bromide with double-strand polynucleotides |
| title_fullStr | Fluorescence studies of the interaction of pyrenylmethyl tributylphosphonium bromide with double-strand polynucleotides |
| title_full_unstemmed | Fluorescence studies of the interaction of pyrenylmethyl tributylphosphonium bromide with double-strand polynucleotides |
| title_short | Fluorescence studies of the interaction of pyrenylmethyl tributylphosphonium bromide with double-strand polynucleotides |
| title_sort | Fluorescence studies of the interaction of pyrenylmethyl tributylphosphonium bromide with double-strand polynucleotides |
| topic | Polynucleotides Fluorescence Pyrene derivatives |
| topic_facet | Polynucleotides Fluorescence Pyrene derivatives |
| url | https://hdl.handle.net/1822/5141 |
| visible | 1 |