Publicação
Advances and drawbacks of the adaptation to serum-free culture of CHO-K1 cells for monoclonal antibody production
| Resumo: | Currently, mammalian cell technology has become the focus of biopharmaceutical production, with strict regulatory scrutiny of the techniques employed. Major concerns about the presence of animal-derived components in the culture media led to the development of serum-free (SF) culture processes. However, cell adaptation to SF conditions is still a major challenge and limiting step of process development. Thus, this study aims to assess the impact of SF adaptation on monoclonal antibody production (mAb), identify the most critical steps of cell adaptation to the SF EX-CELL medium, and create basic process guidelines.. The success of SF adaptation was dependent on critical steps that included: accentuated cell sensitivity to common culture procedures (centrifugation, trypsinization); initial cell concentration; time given at each step of serum-reduction; and, most importantly, medium supplements used to support adaptation. Indeed, only one of the five supplement combinations assessed (rhinsulin, ammonium metavanadate, nickel chloride and stannous chloride) succeeded for the CHO-K1 cell line used. This work also revealed that the chemically-defined EX-CELL medium benefits mAb production in comparison with the general purpose Dulbecco's Modified Eagle's Medium, but the complete removal of serum attenuates these positive effects. |
|---|---|
| Autores principais: | Rodrigues, E. |
| Outros Autores: | Costa, A. R.; Henriques, Mariana; Cunnah, Philip; Melton, David; Azeredo, Joana; Oliveira, Rosário |
| Assunto: | Chinese hamster ovary cells Monoclonal antibody Production Serum-free medium Trace elements Cell adaptation |
| Ano: | 2013 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867438928862117888 |
|---|---|
| author | Rodrigues, E. |
| author2 | Costa, A. R. Henriques, Mariana Cunnah, Philip Melton, David Azeredo, Joana Oliveira, Rosário |
| author2_role | author author author author author author |
| author_facet | Rodrigues, E. Costa, A. R. Henriques, Mariana Cunnah, Philip Melton, David Azeredo, Joana Oliveira, Rosário |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Rodrigues, E.\"},{\"Person.name\":\"Costa, A. R.\"},{\"Person.name\":\"Henriques, Mariana\"},{\"Person.name\":\"Cunnah, Philip\"},{\"Person.name\":\"Melton, David\"},{\"Person.name\":\"Azeredo, Joana\"},{\"Person.name\":\"Oliveira, Rosário\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Rodrigues, E. Costa, A. R. Henriques, Mariana Cunnah, Philip Melton, David Azeredo, Joana Oliveira, Rosário |
| datacite.date.Accepted.fl_str_mv | 2013-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2013-10-04T14:35:54Z |
| datacite.date.embargoed.fl_str_mv | 2013-10-04T14:35:54Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Chinese hamster ovary cells Monoclonal antibody Production Serum-free medium Trace elements Cell adaptation |
| datacite.titles.title.fl_str_mv | Advances and drawbacks of the adaptation to serum-free culture of CHO-K1 cells for monoclonal antibody production |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Rodrigues, E. Costa, A. R. Henriques, Mariana Cunnah, Philip Melton, David Azeredo, Joana Oliveira, Rosário |
| dc.date.Accepted.fl_str_mv | 2013-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2013-10-04T14:35:54Z |
| dc.date.embargoed.fl_str_mv | 2013-10-04T14:35:54Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/25501 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Springer |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Chinese hamster ovary cells Monoclonal antibody Production Serum-free medium Trace elements Cell adaptation |
| dc.title.fl_str_mv | Advances and drawbacks of the adaptation to serum-free culture of CHO-K1 cells for monoclonal antibody production |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Currently, mammalian cell technology has become the focus of biopharmaceutical production, with strict regulatory scrutiny of the techniques employed. Major concerns about the presence of animal-derived components in the culture media led to the development of serum-free (SF) culture processes. However, cell adaptation to SF conditions is still a major challenge and limiting step of process development. Thus, this study aims to assess the impact of SF adaptation on monoclonal antibody production (mAb), identify the most critical steps of cell adaptation to the SF EX-CELL medium, and create basic process guidelines.. The success of SF adaptation was dependent on critical steps that included: accentuated cell sensitivity to common culture procedures (centrifugation, trypsinization); initial cell concentration; time given at each step of serum-reduction; and, most importantly, medium supplements used to support adaptation. Indeed, only one of the five supplement combinations assessed (rhinsulin, ammonium metavanadate, nickel chloride and stannous chloride) succeeded for the CHO-K1 cell line used. This work also revealed that the chemically-defined EX-CELL medium benefits mAb production in comparison with the general purpose Dulbecco's Modified Eagle's Medium, but the complete removal of serum attenuates these positive effects. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/c207d9c7-9540-4357-8904-1923c568a26c/download |
| id | rum_832f9fe3dcd8a879d7ec964cc4b53d2d |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/25501 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/25501 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Rodrigues, E. Costa, A. R. Henriques, Mariana Cunnah, Philip Melton, David Azeredo, Joana Oliveira, Rosário |
| publishDate | 2013 |
| publisher.none.fl_str_mv | Springer |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engSpringerporCurrently, mammalian cell technology has become the focus of biopharmaceutical production, with strict regulatory scrutiny of the techniques employed. Major concerns about the presence of animal-derived components in the culture media led to the development of serum-free (SF) culture processes. However, cell adaptation to SF conditions is still a major challenge and limiting step of process development. Thus, this study aims to assess the impact of SF adaptation on monoclonal antibody production (mAb), identify the most critical steps of cell adaptation to the SF EX-CELL medium, and create basic process guidelines.. The success of SF adaptation was dependent on critical steps that included: accentuated cell sensitivity to common culture procedures (centrifugation, trypsinization); initial cell concentration; time given at each step of serum-reduction; and, most importantly, medium supplements used to support adaptation. Indeed, only one of the five supplement combinations assessed (rhinsulin, ammonium metavanadate, nickel chloride and stannous chloride) succeeded for the CHO-K1 cell line used. This work also revealed that the chemically-defined EX-CELL medium benefits mAb production in comparison with the general purpose Dulbecco's Modified Eagle's Medium, but the complete removal of serum attenuates these positive effects.application/pdfporAdvances and drawbacks of the adaptation to serum-free culture of CHO-K1 cells for monoclonal antibody productionRodrigues, E.Costa, A. R.Henriques, MarianaCunnah, PhilipMelton, DavidAzeredo, JoanaOliveira, RosárioHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptPMID23306891ISSNIsPartOf0273-2289DOIIsPartOf10.1007/s12010-012-0068-z2013-10-04T14:35:54Z20132013-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/25501http://purl.org/coar/access_right/c_abf2open accessChinese hamster ovary cellsMonoclonal antibodyProductionSerum-free mediumTrace elementsCell adaptation397430 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/c207d9c7-9540-4357-8904-1923c568a26c/download |
| spellingShingle | Advances and drawbacks of the adaptation to serum-free culture of CHO-K1 cells for monoclonal antibody production Rodrigues, E. Chinese hamster ovary cells Monoclonal antibody Production Serum-free medium Trace elements Cell adaptation |
| status | SINGLETON |
| subject.fl_str_mv | Chinese hamster ovary cells Monoclonal antibody Production Serum-free medium Trace elements Cell adaptation |
| title | Advances and drawbacks of the adaptation to serum-free culture of CHO-K1 cells for monoclonal antibody production |
| title_full | Advances and drawbacks of the adaptation to serum-free culture of CHO-K1 cells for monoclonal antibody production |
| title_fullStr | Advances and drawbacks of the adaptation to serum-free culture of CHO-K1 cells for monoclonal antibody production |
| title_full_unstemmed | Advances and drawbacks of the adaptation to serum-free culture of CHO-K1 cells for monoclonal antibody production |
| title_short | Advances and drawbacks of the adaptation to serum-free culture of CHO-K1 cells for monoclonal antibody production |
| title_sort | Advances and drawbacks of the adaptation to serum-free culture of CHO-K1 cells for monoclonal antibody production |
| topic | Chinese hamster ovary cells Monoclonal antibody Production Serum-free medium Trace elements Cell adaptation |
| topic_facet | Chinese hamster ovary cells Monoclonal antibody Production Serum-free medium Trace elements Cell adaptation |
| url | https://hdl.handle.net/1822/25501 |
| visible | 1 |