Publicação
Multivariable optimization process of heterotrophic growth of Chlorella vulgaris
| Resumo: | Microalgae have received increasing attention as one of the most promising feedstocks in the development of new healthier food products and different strategies have been attempted to improve their growth. However, the high production costs and low productivities, commonly associated with photoautotrophic growths, are still a big challenge. In this study, a two-step optimization strategy was carried out in order to maximize the biomass production of a Chlorella vulgaris strain used at industrial scale under heterotrophic conditions. From a total of 24 independent variables, which were studied simultaneously, 10 have presented a positive effect over Xmax, while the remaining have shown to be negative. The amount of (NH4)2SO4 (6.3 g L1), MgSO4·7H2O (0.7 g L1), and C6H12O6 (50% w/v) in the culture medium has revealed to be the only factors with a significant impact on biomass concentration, with optimum values of 25.5, 64.6, and 75 ml.L1, respectively. The optimized medium resulted in an improvement of the Xmax by 99.6% when compared to the growth medium applied at industrial scale, proving the success of this strategy. Additionally, the carbohydrates production was enhanced by 48.0%. |
|---|---|
| Autores principais: | Geada, Pedro |
| Outros Autores: | Francisco, Diogo; Pereira, Francisco; Filipe Maciel; Madureira, Leandro; Barros, Ana; Silva, Joana L.; Vicente, A. A.; Teixeira, J. A. |
| Assunto: | Microalgae Design of experiment tools Biomass concentration maximization Growth medium composition Two-step optimization process Synergistic interactions Biomass concentration  maximization |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439205605441536 |
|---|---|
| author | Geada, Pedro |
| author2 | Francisco, Diogo Pereira, Francisco Filipe Maciel Madureira, Leandro Barros, Ana Silva, Joana L. Vicente, A. A. Teixeira, J. A. |
| author2_role | author author author author author author author author |
| author_facet | Geada, Pedro Francisco, Diogo Pereira, Francisco Filipe Maciel Madureira, Leandro Barros, Ana Silva, Joana L. Vicente, A. A. Teixeira, J. A. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Geada, Pedro\"},{\"Person.name\":\"Francisco, Diogo\"},{\"Person.name\":\"Pereira, Francisco\"},{\"Person.name\":\"Filipe Maciel\"},{\"Person.name\":\"Madureira, Leandro\"},{\"Person.name\":\"Barros, Ana\"},{\"Person.name\":\"Silva, Joana L.\"},{\"Person.name\":\"Vicente, A. A.\"},{\"Person.name\":\"Teixeira, J. A.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Geada, Pedro Francisco, Diogo Pereira, Francisco Filipe Maciel Madureira, Leandro Barros, Ana Silva, Joana L. Vicente, A. A. Teixeira, J. A. |
| datacite.date.Accepted.fl_str_mv | 2023-03-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-01-11T10:28:12Z |
| datacite.date.embargoed.fl_str_mv | 2023-01-11T10:28:12Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Microalgae Design of experiment tools Biomass concentration maximization Growth medium composition Two-step optimization process Synergistic interactions Biomass concentration  maximization |
| datacite.titles.title.fl_str_mv | Multivariable optimization process of heterotrophic growth of Chlorella vulgaris |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Geada, Pedro Francisco, Diogo Pereira, Francisco Filipe Maciel Madureira, Leandro Barros, Ana Silva, Joana L. Vicente, A. A. Teixeira, J. A. |
| dc.date.Accepted.fl_str_mv | 2023-03-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-01-11T10:28:12Z |
| dc.date.embargoed.fl_str_mv | 2023-01-11T10:28:12Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/81709 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.rights.rights.copyright.fl_str_mv | openAccess |
| dc.subject.none.fl_str_mv | Microalgae Design of experiment tools Biomass concentration maximization Growth medium composition Two-step optimization process Synergistic interactions Biomass concentration  maximization |
| dc.title.fl_str_mv | Multivariable optimization process of heterotrophic growth of Chlorella vulgaris |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Microalgae have received increasing attention as one of the most promising feedstocks in the development of new healthier food products and different strategies have been attempted to improve their growth. However, the high production costs and low productivities, commonly associated with photoautotrophic growths, are still a big challenge. In this study, a two-step optimization strategy was carried out in order to maximize the biomass production of a Chlorella vulgaris strain used at industrial scale under heterotrophic conditions. From a total of 24 independent variables, which were studied simultaneously, 10 have presented a positive effect over Xmax, while the remaining have shown to be negative. The amount of (NH4)2SO4 (6.3 g L1), MgSO4·7H2O (0.7 g L1), and C6H12O6 (50% w/v) in the culture medium has revealed to be the only factors with a significant impact on biomass concentration, with optimum values of 25.5, 64.6, and 75 ml.L1, respectively. The optimized medium resulted in an improvement of the Xmax by 99.6% when compared to the growth medium applied at industrial scale, proving the success of this strategy. Additionally, the carbohydrates production was enhanced by 48.0%. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/f1889f53-7a21-4648-91ae-48429551cc62/download |
| id | rum_84cff3093445984b4e2e9366e5edf11e |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/81709 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/81709 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Geada, Pedro Francisco, Diogo Pereira, Francisco Filipe Maciel Madureira, Leandro Barros, Ana Silva, Joana L. Vicente, A. A. Teixeira, J. A. |
| publishDate | 2023 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engElsevierporMicroalgae have received increasing attention as one of the most promising feedstocks in the development of new healthier food products and different strategies have been attempted to improve their growth. However, the high production costs and low productivities, commonly associated with photoautotrophic growths, are still a big challenge. In this study, a two-step optimization strategy was carried out in order to maximize the biomass production of a Chlorella vulgaris strain used at industrial scale under heterotrophic conditions. From a total of 24 independent variables, which were studied simultaneously, 10 have presented a positive effect over Xmax, while the remaining have shown to be negative. The amount of (NH4)2SO4 (6.3 g L1), MgSO4·7H2O (0.7 g L1), and C6H12O6 (50% w/v) in the culture medium has revealed to be the only factors with a significant impact on biomass concentration, with optimum values of 25.5, 64.6, and 75 ml.L1, respectively. The optimized medium resulted in an improvement of the Xmax by 99.6% when compared to the growth medium applied at industrial scale, proving the success of this strategy. Additionally, the carbohydrates production was enhanced by 48.0%.application/pdfporMultivariable optimization process of heterotrophic growth of Chlorella vulgarisGeada, PedroFrancisco, DiogoPereira, FranciscoFilipe MacielMadureira, LeandroBarros, AnaSilva, Joana L.Vicente, A. A.Teixeira, J. A.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONGeada, P., Francisco, D., Pereira, F., Maciel, F., Madureira, L., Barros, A., … Teixeira, J. A. (2023, March). Multivariable optimization process of heterotrophic growth of Chlorella vulgaris. Food and Bioproducts Processing. Elsevier BV. http://doi.org/10.1016/j.fbp.2022.12.004ISSNIsPartOf0960-3085DOIIsPartOf10.1016/j.fbp.2022.12.0042023-01-11T10:28:12Z2023-032023-01-04T18:26:59Z2023-03-01T00:00:00ZHandlehttps://hdl.handle.net/1822/81709http://purl.org/coar/access_right/c_abf2open accessMicroalgaeDesign of experiment toolsBiomass concentration maximizationGrowth medium compositionTwo-step optimization processSynergistic interactionsBiomass concentration maximization2180956 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2023-03http://creativecommons.org/licenses/by-nc-nd/4.0/openAccesshttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/f1889f53-7a21-4648-91ae-48429551cc62/download |
| spellingShingle | Multivariable optimization process of heterotrophic growth of Chlorella vulgaris Geada, Pedro Microalgae Design of experiment tools Biomass concentration maximization Growth medium composition Two-step optimization process Synergistic interactions Biomass concentration  maximization |
| status | SINGLETON |
| subject.fl_str_mv | Microalgae Design of experiment tools Biomass concentration maximization Growth medium composition Two-step optimization process Synergistic interactions Biomass concentration  maximization |
| title | Multivariable optimization process of heterotrophic growth of Chlorella vulgaris |
| title_full | Multivariable optimization process of heterotrophic growth of Chlorella vulgaris |
| title_fullStr | Multivariable optimization process of heterotrophic growth of Chlorella vulgaris |
| title_full_unstemmed | Multivariable optimization process of heterotrophic growth of Chlorella vulgaris |
| title_short | Multivariable optimization process of heterotrophic growth of Chlorella vulgaris |
| title_sort | Multivariable optimization process of heterotrophic growth of Chlorella vulgaris |
| topic | Microalgae Design of experiment tools Biomass concentration maximization Growth medium composition Two-step optimization process Synergistic interactions Biomass concentration  maximization |
| topic_facet | Microalgae Design of experiment tools Biomass concentration maximization Growth medium composition Two-step optimization process Synergistic interactions Biomass concentration  maximization |
| url | https://hdl.handle.net/1822/81709 |
| visible | 1 |