Publicação
In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient
| Resumo: | Until now the prebiotic potential of pure trisaccharide raffinose on human health assessed through high-throughput sequencing remains poorly investigated. In this work, an in vitro model using human fecal inocula of two healthy volunteers (D1 and D2) was used to study the prebiotic potential of raffinose and compare it with the well-stablished and commercial prebiotic lactulose. The intestinal microbiota showed preference for raffinose as substrate showing the highest consumption value at 48h (96.0±0.9% D1 and 95.3±0.7% D2). The fermentation of raffinose decreased the medium pH, the ammonia concentration and the relative amount of Proteobacteria, while increasing the total production of lactate and short chain fatty acids (129.9±2.6mmol/L D1 and 179.6±0.6mmol/L D2), CO2 (10.8±0.8 mmol/Lmedium D1 and 5.2±0.3 mmol/Lmedium D2) and the relative amount of Bifidobacterium and Lactobacillus. This study suggests that raffinose holds potential functional properties for human health. |
|---|---|
| Autores principais: | Amorim, Cláudia |
| Outros Autores: | Silvério, Sara Isabel Cruz; Cardoso, B.; Alves, J. I.; Pereira, M. A.; Rodrigues, L. R. |
| Assunto: | Prebiotic Lactulose Raffinose Human fecal inocula in vitro fermentation |
| Ano: | 2020 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866875412561264640 |
|---|---|
| author | Amorim, Cláudia |
| author2 | Silvério, Sara Isabel Cruz Cardoso, B. Alves, J. I. Pereira, M. A. Rodrigues, L. R. |
| author2_role | author author author author author |
| author_facet | Amorim, Cláudia Silvério, Sara Isabel Cruz Cardoso, B. Alves, J. I. Pereira, M. A. Rodrigues, L. R. |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Amorim, Cláudia\"},{\"Person.name\":\"Silvério, Sara Isabel Cruz\"},{\"Person.name\":\"Cardoso, B.\"},{\"Person.name\":\"Alves, J. I.\"},{\"Person.name\":\"Pereira, M. A.\"},{\"Person.name\":\"Rodrigues, L. R.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Amorim, Cláudia Silvério, Sara Isabel Cruz Cardoso, B. Alves, J. I. Pereira, M. A. Rodrigues, L. R. |
| datacite.date.Accepted.fl_str_mv | 2020-05-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2020-06-12T10:50:55Z |
| datacite.date.embargoed.fl_str_mv | 2020-06-12T10:50:55Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Prebiotic Lactulose Raffinose Human fecal inocula in vitro fermentation |
| datacite.titles.title.fl_str_mv | In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Amorim, Cláudia Silvério, Sara Isabel Cruz Cardoso, B. Alves, J. I. Pereira, M. A. Rodrigues, L. R. |
| dc.date.Accepted.fl_str_mv | 2020-05-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2020-06-12T10:50:55Z |
| dc.date.embargoed.fl_str_mv | 2020-06-12T10:50:55Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/65610 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Prebiotic Lactulose Raffinose Human fecal inocula in vitro fermentation |
| dc.title.fl_str_mv | In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Until now the prebiotic potential of pure trisaccharide raffinose on human health assessed through high-throughput sequencing remains poorly investigated. In this work, an in vitro model using human fecal inocula of two healthy volunteers (D1 and D2) was used to study the prebiotic potential of raffinose and compare it with the well-stablished and commercial prebiotic lactulose. The intestinal microbiota showed preference for raffinose as substrate showing the highest consumption value at 48h (96.0±0.9% D1 and 95.3±0.7% D2). The fermentation of raffinose decreased the medium pH, the ammonia concentration and the relative amount of Proteobacteria, while increasing the total production of lactate and short chain fatty acids (129.9±2.6mmol/L D1 and 179.6±0.6mmol/L D2), CO2 (10.8±0.8 mmol/Lmedium D1 and 5.2±0.3 mmol/Lmedium D2) and the relative amount of Bifidobacterium and Lactobacillus. This study suggests that raffinose holds potential functional properties for human health. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/8d83f3e6-5624-4dfe-9866-5a1de971780b/download |
| id | rum_8528417b87aa0ebefdb9c4a545dfd4b5 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/65610 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/65610 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Amorim, Cláudia Silvério, Sara Isabel Cruz Cardoso, B. Alves, J. I. Pereira, M. A. Rodrigues, L. R. |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engElsevierporUntil now the prebiotic potential of pure trisaccharide raffinose on human health assessed through high-throughput sequencing remains poorly investigated. In this work, an in vitro model using human fecal inocula of two healthy volunteers (D1 and D2) was used to study the prebiotic potential of raffinose and compare it with the well-stablished and commercial prebiotic lactulose. The intestinal microbiota showed preference for raffinose as substrate showing the highest consumption value at 48h (96.0±0.9% D1 and 95.3±0.7% D2). The fermentation of raffinose decreased the medium pH, the ammonia concentration and the relative amount of Proteobacteria, while increasing the total production of lactate and short chain fatty acids (129.9±2.6mmol/L D1 and 179.6±0.6mmol/L D2), CO2 (10.8±0.8 mmol/Lmedium D1 and 5.2±0.3 mmol/Lmedium D2) and the relative amount of Bifidobacterium and Lactobacillus. This study suggests that raffinose holds potential functional properties for human health.application/pdfporIn vitro fermentation of raffinose to unravel its potential as prebiotic ingredientAmorim, CláudiaSilvério, Sara Isabel CruzCardoso, B.Alves, J. I.Pereira, M. A.Rodrigues, L. R.HostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf0023-6438DOIIsPartOf10.1016/j.lwt.2020.1093222020-06-12T10:50:55Z2020-052020-04-04T12:25:06Z2020-05-01T00:00:00ZHandlehttps://hdl.handle.net/1822/65610http://purl.org/coar/access_right/c_abf2open accessPrebioticLactuloseRaffinoseHuman fecal inoculain vitro fermentation1621537 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/8d83f3e6-5624-4dfe-9866-5a1de971780b/download |
| spellingShingle | In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient Amorim, Cláudia Prebiotic Lactulose Raffinose Human fecal inocula in vitro fermentation |
| status | SINGLETON |
| subject.fl_str_mv | Prebiotic Lactulose Raffinose Human fecal inocula in vitro fermentation |
| title | In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient |
| title_full | In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient |
| title_fullStr | In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient |
| title_full_unstemmed | In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient |
| title_short | In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient |
| title_sort | In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient |
| topic | Prebiotic Lactulose Raffinose Human fecal inocula in vitro fermentation |
| topic_facet | Prebiotic Lactulose Raffinose Human fecal inocula in vitro fermentation |
| url | https://hdl.handle.net/1822/65610 |
| visible | 1 |