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Effect of antioxidant-rich propolis and bee pollen extracts against D-glucose induced Type 2 Diabetes in rats
| Resumo: | The present study was designed to investigate the preventive effect of propolis, bee pollen and their combination on Type 2 diabetes induced by D-glucose in rats. The study was carried out by feeding daily two concentrations (100 and 200 mg/Kg BW) of propolis or bee pollen (or their combination to normal (non-diabetic) and diabetic rats for a period of 16 weeks. In vivo biochemical changes associated to diabetes are induced by drinking a solution containing 10% of D-glucose (diabetic rats). The in vitro antioxidant activity was also evaluated and the chemical composition of propolis and bee pollen extracts was determined by UHPLC-DAD. Phytochemical composition of propolis and bee pollen revealed the presence of several natural antioxidants, such as hydroxycinnamic acids, hydroxybenzoic acids, flavonoids, flavan-3-ols and stilbens. The major antioxidant compound present in propolis was Naringin (290.19±0.2 mg/Kg) and in bee pollen was apigenin (162.85±17.7 mg/Kg). These results have been related with a high antioxidant activity, more intense in propolis extract. In rats, the administration of D-glucose had induced hyperglycemia (13.2 ± 0.82 mmol/L), increased plasmatic insulin levels (25.10 ± 2.12 U/L) and HOMA-IR index (14.72 ± 0.85) accompanied with dyslipidemia, elevation of hepatic enzyme levels, and a change in both serum renal biomarkers and plasmatic calcium. The co-administration of propolis and bee pollen extracts alone or in combination restored these biochemical parameters and attenuated the deleterious effects of D-glucose on liver and kidney functions. Furthermore, these effects were better attenuated in the combined therapy-prevented diabetic rats. Hence, it is possible to conclude that propolis and bee pollen can be used as a preventive natural product against diabetes induced dyslipidemia and hepato-renal damage. |
|---|---|
| Autores principais: | Hassan Laaroussi |
| Outros Autores: | Bakour, Meryem; Ousaaid, Driss; Aboulghazi, Abderrazak; Ferreira-Santos, P.; Genisheva, Zlatina; Teixeira, J. A.; Lyoussi, Badiaa |
| Assunto: | Propolis Bee pollen Type 2 diabetes D-glucose Dyslipidemia Hepato-renal damage |
| Ano: | 2020 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866269588609564672 |
|---|---|
| author | Hassan Laaroussi |
| author2 | Bakour, Meryem Ousaaid, Driss Aboulghazi, Abderrazak Ferreira-Santos, P. Genisheva, Zlatina Teixeira, J. A. Lyoussi, Badiaa |
| author2_role | author author author author author author author |
| author_facet | Hassan Laaroussi Bakour, Meryem Ousaaid, Driss Aboulghazi, Abderrazak Ferreira-Santos, P. Genisheva, Zlatina Teixeira, J. A. Lyoussi, Badiaa |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Hassan Laaroussi\"},{\"Person.name\":\"Bakour, Meryem\"},{\"Person.name\":\"Ousaaid, Driss\"},{\"Person.name\":\"Aboulghazi, Abderrazak\"},{\"Person.name\":\"Ferreira-Santos, P.\"},{\"Person.name\":\"Genisheva, Zlatina\"},{\"Person.name\":\"Teixeira, J. A.\"},{\"Person.name\":\"Lyoussi, Badiaa\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Hassan Laaroussi Bakour, Meryem Ousaaid, Driss Aboulghazi, Abderrazak Ferreira-Santos, P. Genisheva, Zlatina Teixeira, J. A. Lyoussi, Badiaa |
| datacite.date.Accepted.fl_str_mv | 2020-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2020-10-24T13:10:41Z |
| datacite.date.embargoed.fl_str_mv | 2020-10-24T13:10:41Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Propolis Bee pollen Type 2 diabetes D-glucose Dyslipidemia Hepato-renal damage |
| datacite.titles.title.fl_str_mv | Effect of antioxidant-rich propolis and bee pollen extracts against D-glucose induced Type 2 Diabetes in rats |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Hassan Laaroussi Bakour, Meryem Ousaaid, Driss Aboulghazi, Abderrazak Ferreira-Santos, P. Genisheva, Zlatina Teixeira, J. A. Lyoussi, Badiaa |
| dc.date.Accepted.fl_str_mv | 2020-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2020-10-24T13:10:41Z |
| dc.date.embargoed.fl_str_mv | 2020-10-24T13:10:41Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/67681 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Propolis Bee pollen Type 2 diabetes D-glucose Dyslipidemia Hepato-renal damage |
| dc.title.fl_str_mv | Effect of antioxidant-rich propolis and bee pollen extracts against D-glucose induced Type 2 Diabetes in rats |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The present study was designed to investigate the preventive effect of propolis, bee pollen and their combination on Type 2 diabetes induced by D-glucose in rats. The study was carried out by feeding daily two concentrations (100 and 200 mg/Kg BW) of propolis or bee pollen (or their combination to normal (non-diabetic) and diabetic rats for a period of 16 weeks. In vivo biochemical changes associated to diabetes are induced by drinking a solution containing 10% of D-glucose (diabetic rats). The in vitro antioxidant activity was also evaluated and the chemical composition of propolis and bee pollen extracts was determined by UHPLC-DAD. Phytochemical composition of propolis and bee pollen revealed the presence of several natural antioxidants, such as hydroxycinnamic acids, hydroxybenzoic acids, flavonoids, flavan-3-ols and stilbens. The major antioxidant compound present in propolis was Naringin (290.19±0.2 mg/Kg) and in bee pollen was apigenin (162.85±17.7 mg/Kg). These results have been related with a high antioxidant activity, more intense in propolis extract. In rats, the administration of D-glucose had induced hyperglycemia (13.2 ± 0.82 mmol/L), increased plasmatic insulin levels (25.10 ± 2.12 U/L) and HOMA-IR index (14.72 ± 0.85) accompanied with dyslipidemia, elevation of hepatic enzyme levels, and a change in both serum renal biomarkers and plasmatic calcium. The co-administration of propolis and bee pollen extracts alone or in combination restored these biochemical parameters and attenuated the deleterious effects of D-glucose on liver and kidney functions. Furthermore, these effects were better attenuated in the combined therapy-prevented diabetic rats. Hence, it is possible to conclude that propolis and bee pollen can be used as a preventive natural product against diabetes induced dyslipidemia and hepato-renal damage. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/5ee2ec5b-f288-425a-b956-9fcc79c4ba22/download |
| id | rum_8bbb50fb94f67d325909d1ce210fcdca |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/67681 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/67681 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Hassan Laaroussi Bakour, Meryem Ousaaid, Driss Aboulghazi, Abderrazak Ferreira-Santos, P. Genisheva, Zlatina Teixeira, J. A. Lyoussi, Badiaa |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engElsevierporThe present study was designed to investigate the preventive effect of propolis, bee pollen and their combination on Type 2 diabetes induced by D-glucose in rats. The study was carried out by feeding daily two concentrations (100 and 200 mg/Kg BW) of propolis or bee pollen (or their combination to normal (non-diabetic) and diabetic rats for a period of 16 weeks. In vivo biochemical changes associated to diabetes are induced by drinking a solution containing 10% of D-glucose (diabetic rats). The in vitro antioxidant activity was also evaluated and the chemical composition of propolis and bee pollen extracts was determined by UHPLC-DAD. Phytochemical composition of propolis and bee pollen revealed the presence of several natural antioxidants, such as hydroxycinnamic acids, hydroxybenzoic acids, flavonoids, flavan-3-ols and stilbens. The major antioxidant compound present in propolis was Naringin (290.19±0.2 mg/Kg) and in bee pollen was apigenin (162.85±17.7 mg/Kg). These results have been related with a high antioxidant activity, more intense in propolis extract. In rats, the administration of D-glucose had induced hyperglycemia (13.2 ± 0.82 mmol/L), increased plasmatic insulin levels (25.10 ± 2.12 U/L) and HOMA-IR index (14.72 ± 0.85) accompanied with dyslipidemia, elevation of hepatic enzyme levels, and a change in both serum renal biomarkers and plasmatic calcium. The co-administration of propolis and bee pollen extracts alone or in combination restored these biochemical parameters and attenuated the deleterious effects of D-glucose on liver and kidney functions. Furthermore, these effects were better attenuated in the combined therapy-prevented diabetic rats. Hence, it is possible to conclude that propolis and bee pollen can be used as a preventive natural product against diabetes induced dyslipidemia and hepato-renal damage.application/pdfporEffect of antioxidant-rich propolis and bee pollen extracts against D-glucose induced Type 2 Diabetes in ratsHassan LaaroussiBakour, MeryemOusaaid, DrissAboulghazi, AbderrazakFerreira-Santos, P.Genisheva, ZlatinaTeixeira, J. A.Lyoussi, BadiaaHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf0963-9969DOIIsPartOf10.1016/j.foodres.2020.1098022020-10-24T13:10:41Z20202020-10-24T10:14:49Z2020-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/67681http://purl.org/coar/access_right/c_abf2open accessPropolisBee pollenType 2 diabetesD-glucoseDyslipidemiaHepato-renal damage2210859 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/5ee2ec5b-f288-425a-b956-9fcc79c4ba22/download |
| spellingShingle | Effect of antioxidant-rich propolis and bee pollen extracts against D-glucose induced Type 2 Diabetes in rats Hassan Laaroussi Propolis Bee pollen Type 2 diabetes D-glucose Dyslipidemia Hepato-renal damage |
| status | SINGLETON |
| subject.fl_str_mv | Propolis Bee pollen Type 2 diabetes D-glucose Dyslipidemia Hepato-renal damage |
| title | Effect of antioxidant-rich propolis and bee pollen extracts against D-glucose induced Type 2 Diabetes in rats |
| title_full | Effect of antioxidant-rich propolis and bee pollen extracts against D-glucose induced Type 2 Diabetes in rats |
| title_fullStr | Effect of antioxidant-rich propolis and bee pollen extracts against D-glucose induced Type 2 Diabetes in rats |
| title_full_unstemmed | Effect of antioxidant-rich propolis and bee pollen extracts against D-glucose induced Type 2 Diabetes in rats |
| title_short | Effect of antioxidant-rich propolis and bee pollen extracts against D-glucose induced Type 2 Diabetes in rats |
| title_sort | Effect of antioxidant-rich propolis and bee pollen extracts against D-glucose induced Type 2 Diabetes in rats |
| topic | Propolis Bee pollen Type 2 diabetes D-glucose Dyslipidemia Hepato-renal damage |
| topic_facet | Propolis Bee pollen Type 2 diabetes D-glucose Dyslipidemia Hepato-renal damage |
| url | https://hdl.handle.net/1822/67681 |
| visible | 1 |