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MALDI-TOF MS to identify the pineapple pathogen Fusarium guttiforme and its antagonist Trichoderma asperellum on decayed pineapple

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Resumo:Matrix-assisted laser desorption⁄ionisation time-of flight mass spectrometry (MALDI-TOF MS) has been used to identify some Fusarium and Trichoderma species, but early detection of fungal diseases by this technique has not yet been fully addressed. In this study, MALDI-TOF MS was tested to identify F. guttiforme on pineapple side shoots in situ. The efficacy of filamentous fungi for controlling fungal diseases is well documented. However, there is uncertainty whether the biocontrol agent is out growing the pathogen sufficiently to be identified. In this paper, a multistep identification of a plant pathogen (F. guttiforme) and its antagonist (T. asperellum) using MALDI-TOF MS is demonstrated.
Autores principais:Santos, Cledir
Outros Autores:Ventura, José A.; Costa, Hélcio; Fernandes, Patrícia M.B.; Lima, Nelson
Assunto:Fungal ribosomal proteins Fungal early detection MALDI-TOF MS Pineapple Ciências Naturais::Ciências Biológicas
Ano:2015
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso restrito
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho

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