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Application of binary packing for chromatographic separation

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Resumo:Separation of dextran and polyethylene glycol of different molecular mass was performed using a binary packed column of glass beads (size ratio ~ 10) and a binary packed column formed by kieselguhr-G (for thin layer chromatography, Merck) and glass beads as the large size particulate fraction (size ratio ~ 30). In addition, data on the separation of micro-spheres, bacillus and yeast cells using monosized and binary glass beads columns are presented. Obtained results show the advantages of using binary packed columns formed by fine and coarse particles instead of a monosize packing of fine particles. The importance of pore channels tortuosity effect on the separation of shaped microparticles using a binary packing is demonstrated.
Autores principais:Mota, M.
Outros Autores:Teixeira, J. A.; Yelshin, Alexander; Dias, Ricardo P.; Cortez, Susana
Assunto:Chromatography Binary packing Separation Macromolecules Micro-particles
Ano:2005
País:Portugal
Tipo de documento:comunicação em conferência
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho
Descrição
Resumo:Separation of dextran and polyethylene glycol of different molecular mass was performed using a binary packed column of glass beads (size ratio ~ 10) and a binary packed column formed by kieselguhr-G (for thin layer chromatography, Merck) and glass beads as the large size particulate fraction (size ratio ~ 30). In addition, data on the separation of micro-spheres, bacillus and yeast cells using monosized and binary glass beads columns are presented. Obtained results show the advantages of using binary packed columns formed by fine and coarse particles instead of a monosize packing of fine particles. The importance of pore channels tortuosity effect on the separation of shaped microparticles using a binary packing is demonstrated.