Publicação
ColOsH - a dynamic simulator of the human Colon: concept and design
| Resumo: | The impact of functional food and pharmaceuticals on health is intrinsically related with the gastrointestinal (GI) system efficiency. GI simulators are key vehicles for researchers/developers to deliver trustable and reliable results on microbiome modulation and host-microbiota interaction. Changes in the GI microbiome have been largely associated to human diseases such as obesity, diabetes, inflammatory bowel, and cancer. Current dynamic in-vitro GI models allow the simulation of microbiota activity. However, none of them combines dynamic microbiota activity with peristaltic simulation, multi-colon zones and microbiota-host interaction in the same simulator. To address the limitations of current systems, our team is developing a new colon simulator within the ColOsH project (PTDC/BTM-SAL/30071/2017). ColOsH is a novel concept for a dynamic modular Colon system including peristalsis simulation by Oscillatory-flow and Colon-Host interaction. The ColOsH consists of a Colon-Reactor (CR) representative of the three colon zones (ascending, transverse and descending), combined with an integrated Colon-Host (CH) simulator for interaction studies between microbiota and epithelial cells, mucus producing cells and immune system cells. The CR is composed by three oscillatory-flow reactors (OFR) linked in series, carved in a plate. The OFR is a channel shape reactor with periodic sharp constrictions resembling the in-vivo colon villus. The OFR operates under oscillatory-flow mixing, intending to simulate peristalsis. The laminar-flow operation of the OFR will enable working with microbiota and cells in the same simulator. The fouling, generally obtained at low oscillatory condition, will favour bacteria deposition and metabolites exchange with cells. Also, the exceptional OFR mix efficiency is expected to avoid fluid phase separation from solids enabling work with high density immobilized microbiota. To design and setup the OFR operational conditions, a numerical model was developed. The numerical tool mimics flow-field and mix conditions induced by peristalsis on the microbiota. The CH module, consisting in a channel carved in a plate, holds a membrane for cells growth. The CH is installed in the lower part of the CR. The CH and the CR plates are separated by a semi-permeable membrane. The ColOsH will be an important tool for health claims and food safety evaluation. |
|---|---|
| Autores principais: | Nobre, Clarisse |
| Outros Autores: | Ferreira, António; Pires, José C.; Roupar, Dalila; Parente, Inês; Caetano, Ana C.; Martins, Joana T.; Pinheiro, A. C.; Berni, Paulo; Botelho, C. M.; Vicente, A. A.; Teixeira, J. A. |
| Assunto: | Colon simulator Microbiota Dynamic model |
| Ano: | 2019 |
| País: | Portugal |
| Tipo de documento: | outro |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866876176221339648 |
|---|---|
| author | Nobre, Clarisse |
| author2 | Ferreira, António Pires, José C. Roupar, Dalila Parente, Inês Caetano, Ana C. Martins, Joana T. Pinheiro, A. C. Berni, Paulo Botelho, C. M. Vicente, A. A. Teixeira, J. A. |
| author2_role | author author author author author author author author author author author |
| author_facet | Nobre, Clarisse Ferreira, António Pires, José C. Roupar, Dalila Parente, Inês Caetano, Ana C. Martins, Joana T. Pinheiro, A. C. Berni, Paulo Botelho, C. M. Vicente, A. A. Teixeira, J. A. |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Nobre, Clarisse\"},{\"Person.name\":\"Ferreira, António\"},{\"Person.name\":\"Pires, José C.\"},{\"Person.name\":\"Roupar, Dalila\"},{\"Person.name\":\"Parente, Inês\"},{\"Person.name\":\"Caetano, Ana C.\"},{\"Person.name\":\"Martins, Joana T.\"},{\"Person.name\":\"Pinheiro, A. C.\"},{\"Person.name\":\"Berni, Paulo\"},{\"Person.name\":\"Botelho, C. M.\"},{\"Person.name\":\"Vicente, A. A.\"},{\"Person.name\":\"Teixeira, J. A.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Nobre, Clarisse Ferreira, António Pires, José C. Roupar, Dalila Parente, Inês Caetano, Ana C. Martins, Joana T. Pinheiro, A. C. Berni, Paulo Botelho, C. M. Vicente, A. A. Teixeira, J. A. |
| datacite.date.Accepted.fl_str_mv | 2019-12-05T00:00:00Z |
| datacite.date.available.fl_str_mv | 2025-04-10T08:33:14Z |
| datacite.date.embargoed.fl_str_mv | 2025-04-10T08:33:14Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Colon simulator Microbiota Dynamic model |
| datacite.titles.title.fl_str_mv | ColOsH - a dynamic simulator of the human Colon: concept and design |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Nobre, Clarisse Ferreira, António Pires, José C. Roupar, Dalila Parente, Inês Caetano, Ana C. Martins, Joana T. Pinheiro, A. C. Berni, Paulo Botelho, C. M. Vicente, A. A. Teixeira, J. A. |
| dc.date.Accepted.fl_str_mv | 2019-12-05T00:00:00Z |
| dc.date.available.fl_str_mv | 2025-04-10T08:33:14Z |
| dc.date.embargoed.fl_str_mv | 2025-04-10T08:33:14Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/95298 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Colon simulator Microbiota Dynamic model |
| dc.title.fl_str_mv | ColOsH - a dynamic simulator of the human Colon: concept and design |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_1843 |
| description | The impact of functional food and pharmaceuticals on health is intrinsically related with the gastrointestinal (GI) system efficiency. GI simulators are key vehicles for researchers/developers to deliver trustable and reliable results on microbiome modulation and host-microbiota interaction. Changes in the GI microbiome have been largely associated to human diseases such as obesity, diabetes, inflammatory bowel, and cancer. Current dynamic in-vitro GI models allow the simulation of microbiota activity. However, none of them combines dynamic microbiota activity with peristaltic simulation, multi-colon zones and microbiota-host interaction in the same simulator. To address the limitations of current systems, our team is developing a new colon simulator within the ColOsH project (PTDC/BTM-SAL/30071/2017). ColOsH is a novel concept for a dynamic modular Colon system including peristalsis simulation by Oscillatory-flow and Colon-Host interaction. The ColOsH consists of a Colon-Reactor (CR) representative of the three colon zones (ascending, transverse and descending), combined with an integrated Colon-Host (CH) simulator for interaction studies between microbiota and epithelial cells, mucus producing cells and immune system cells. The CR is composed by three oscillatory-flow reactors (OFR) linked in series, carved in a plate. The OFR is a channel shape reactor with periodic sharp constrictions resembling the in-vivo colon villus. The OFR operates under oscillatory-flow mixing, intending to simulate peristalsis. The laminar-flow operation of the OFR will enable working with microbiota and cells in the same simulator. The fouling, generally obtained at low oscillatory condition, will favour bacteria deposition and metabolites exchange with cells. Also, the exceptional OFR mix efficiency is expected to avoid fluid phase separation from solids enabling work with high density immobilized microbiota. To design and setup the OFR operational conditions, a numerical model was developed. The numerical tool mimics flow-field and mix conditions induced by peristalsis on the microbiota. The CH module, consisting in a channel carved in a plate, holds a membrane for cells growth. The CH is installed in the lower part of the CR. The CH and the CR plates are separated by a semi-permeable membrane. The ColOsH will be an important tool for health claims and food safety evaluation. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | other |
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| identifier.url.fl_str_mv | https://hdl.handle.net/1822/95298 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/95298 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Nobre, Clarisse Ferreira, António Pires, José C. Roupar, Dalila Parente, Inês Caetano, Ana C. Martins, Joana T. Pinheiro, A. C. Berni, Paulo Botelho, C. M. Vicente, A. A. Teixeira, J. A. |
| publishDate | 2019 |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engporThe impact of functional food and pharmaceuticals on health is intrinsically related with the gastrointestinal (GI) system efficiency. GI simulators are key vehicles for researchers/developers to deliver trustable and reliable results on microbiome modulation and host-microbiota interaction. Changes in the GI microbiome have been largely associated to human diseases such as obesity, diabetes, inflammatory bowel, and cancer. Current dynamic in-vitro GI models allow the simulation of microbiota activity. However, none of them combines dynamic microbiota activity with peristaltic simulation, multi-colon zones and microbiota-host interaction in the same simulator. To address the limitations of current systems, our team is developing a new colon simulator within the ColOsH project (PTDC/BTM-SAL/30071/2017). ColOsH is a novel concept for a dynamic modular Colon system including peristalsis simulation by Oscillatory-flow and Colon-Host interaction. The ColOsH consists of a Colon-Reactor (CR) representative of the three colon zones (ascending, transverse and descending), combined with an integrated Colon-Host (CH) simulator for interaction studies between microbiota and epithelial cells, mucus producing cells and immune system cells. The CR is composed by three oscillatory-flow reactors (OFR) linked in series, carved in a plate. The OFR is a channel shape reactor with periodic sharp constrictions resembling the in-vivo colon villus. The OFR operates under oscillatory-flow mixing, intending to simulate peristalsis. The laminar-flow operation of the OFR will enable working with microbiota and cells in the same simulator. The fouling, generally obtained at low oscillatory condition, will favour bacteria deposition and metabolites exchange with cells. Also, the exceptional OFR mix efficiency is expected to avoid fluid phase separation from solids enabling work with high density immobilized microbiota. To design and setup the OFR operational conditions, a numerical model was developed. The numerical tool mimics flow-field and mix conditions induced by peristalsis on the microbiota. The CH module, consisting in a channel carved in a plate, holds a membrane for cells growth. The CH is installed in the lower part of the CR. The CH and the CR plates are separated by a semi-permeable membrane. The ColOsH will be an important tool for health claims and food safety evaluation.application/pdfporColOsH - a dynamic simulator of the human Colon: concept and designNobre, ClarisseFerreira, AntónioPires, José C.Roupar, DalilaParente, InêsCaetano, Ana C.Martins, Joana T.Pinheiro, A. C.Berni, PauloBotelho, C. M.Vicente, A. A.Teixeira, J. A.HostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.pt2025-04-10T08:33:14Z2019-12-052025-04-04T10:48:26Z2019-12-05T00:00:00ZHandlehttps://hdl.handle.net/1822/95298http://purl.org/coar/access_right/c_abf2open accessColon simulatorMicrobiotaDynamic model160306 bytesother research producthttp://purl.org/coar/resource_type/c_1843otherhttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/c82af681-3b52-4ae2-96e9-7296ea65bea9/download |
| spellingShingle | ColOsH - a dynamic simulator of the human Colon: concept and design Nobre, Clarisse Colon simulator Microbiota Dynamic model |
| status | SINGLETON |
| subject.fl_str_mv | Colon simulator Microbiota Dynamic model |
| title | ColOsH - a dynamic simulator of the human Colon: concept and design |
| title_full | ColOsH - a dynamic simulator of the human Colon: concept and design |
| title_fullStr | ColOsH - a dynamic simulator of the human Colon: concept and design |
| title_full_unstemmed | ColOsH - a dynamic simulator of the human Colon: concept and design |
| title_short | ColOsH - a dynamic simulator of the human Colon: concept and design |
| title_sort | ColOsH - a dynamic simulator of the human Colon: concept and design |
| topic | Colon simulator Microbiota Dynamic model |
| topic_facet | Colon simulator Microbiota Dynamic model |
| url | https://hdl.handle.net/1822/95298 |
| visible | 1 |