Publicação
Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor
| Resumo: | One of the major challenges in biofuels production from lignocellulosic biomass is the generation of high glucose titers from cellulose in the enzymatic hydrolysis stage of pretreated biomass to guarantee a cost-effective process. Therefore, the enzymatic saccharification on cellulose at high solid loading is an alternative. In this work, the agave bagasse was hydrothermally pretreated and optimized at 194°C/30min, obtaining a pretreated solid rich in cellulose content (>46.46%), and subjected to enzymatic hydrolysis at high solid levels. A horizontal bioreactor was designed for enzyme saccharification at high solid loadings [25% (w/v)]. The bioreactor improved mixing efficiency, with cellulose conversions up to 98% (195.6g/L at 72h). Moreover, mathematical modeling of cellulase deactivation demonstrated that cellulases lose most of their initial activity in the first hours of the reaction. Also, cellulose was characterized by X-ray diffraction, and the pretreated solids were visualized using scanning electron microscopy. |
|---|---|
| Autores principais: | Pino, Marcela Sofía |
| Outros Autores: | Rodríguez-Jasso, Rosa M.; Michelin, Michele; Ruiz, Héctor A. |
| Assunto: | Glucan Glucose Biofuels Autohydrolysis Severity Factor Biorefinery |
| Ano: | 2019 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867438668267913216 |
|---|---|
| author | Pino, Marcela Sofía |
| author2 | Rodríguez-Jasso, Rosa M. Michelin, Michele Ruiz, Héctor A. |
| author2_role | author author author |
| author_facet | Pino, Marcela Sofía Rodríguez-Jasso, Rosa M. Michelin, Michele Ruiz, Héctor A. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Pino, Marcela Sofía\"},{\"Person.name\":\"Rodríguez-Jasso, Rosa M.\"},{\"Person.name\":\"Michelin, Michele\"},{\"Person.name\":\"Ruiz, Héctor A.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Pino, Marcela Sofía Rodríguez-Jasso, Rosa M. Michelin, Michele Ruiz, Héctor A. |
| datacite.date.Accepted.fl_str_mv | 2019-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2019-02-18T11:04:03Z |
| datacite.date.embargoed.fl_str_mv | 2019-02-18T11:04:03Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Glucan Glucose Biofuels Autohydrolysis Severity Factor Biorefinery |
| datacite.titles.title.fl_str_mv | Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Pino, Marcela Sofía Rodríguez-Jasso, Rosa M. Michelin, Michele Ruiz, Héctor A. |
| dc.date.Accepted.fl_str_mv | 2019-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2019-02-18T11:04:03Z |
| dc.date.embargoed.fl_str_mv | 2019-02-18T11:04:03Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/59092 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Glucan Glucose Biofuels Autohydrolysis Severity Factor Biorefinery |
| dc.title.fl_str_mv | Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | One of the major challenges in biofuels production from lignocellulosic biomass is the generation of high glucose titers from cellulose in the enzymatic hydrolysis stage of pretreated biomass to guarantee a cost-effective process. Therefore, the enzymatic saccharification on cellulose at high solid loading is an alternative. In this work, the agave bagasse was hydrothermally pretreated and optimized at 194°C/30min, obtaining a pretreated solid rich in cellulose content (>46.46%), and subjected to enzymatic hydrolysis at high solid levels. A horizontal bioreactor was designed for enzyme saccharification at high solid loadings [25% (w/v)]. The bioreactor improved mixing efficiency, with cellulose conversions up to 98% (195.6g/L at 72h). Moreover, mathematical modeling of cellulase deactivation demonstrated that cellulases lose most of their initial activity in the first hours of the reaction. Also, cellulose was characterized by X-ray diffraction, and the pretreated solids were visualized using scanning electron microscopy. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/44fa4e0d-d9e4-4cdd-b598-0843916a1ba8/download |
| id | rum_9bfe02ca9362c1cd9dca97b54a5e33e5 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/59092 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/59092 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Pino, Marcela Sofía Rodríguez-Jasso, Rosa M. Michelin, Michele Ruiz, Héctor A. |
| publishDate | 2019 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engElsevierporOne of the major challenges in biofuels production from lignocellulosic biomass is the generation of high glucose titers from cellulose in the enzymatic hydrolysis stage of pretreated biomass to guarantee a cost-effective process. Therefore, the enzymatic saccharification on cellulose at high solid loading is an alternative. In this work, the agave bagasse was hydrothermally pretreated and optimized at 194°C/30min, obtaining a pretreated solid rich in cellulose content (>46.46%), and subjected to enzymatic hydrolysis at high solid levels. A horizontal bioreactor was designed for enzyme saccharification at high solid loadings [25% (w/v)]. The bioreactor improved mixing efficiency, with cellulose conversions up to 98% (195.6g/L at 72h). Moreover, mathematical modeling of cellulase deactivation demonstrated that cellulases lose most of their initial activity in the first hours of the reaction. Also, cellulose was characterized by X-ray diffraction, and the pretreated solids were visualized using scanning electron microscopy.application/pdfporEnhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactorPino, Marcela SofíaRodríguez-Jasso, Rosa M.Michelin, MicheleRuiz, Héctor A.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONPino, Marcela; Rodríguez-Jasso, Rosa M.; Michelin, Michele; Ruiz, Héctor A., Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor. Carbohydrate Polymers, 211, 349-359, 2019PMID30824099ISSNIsPartOf0144-8617EISSNIsPartOf0144-8617DOIIsPartOf10.1016/j.carbpol.2019.01.1112019-02-18T11:04:03Z20192019-02-17T12:48:39Z2019-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/59092http://purl.org/coar/access_right/c_abf2open accessGlucanGlucoseBiofuelsAutohydrolysisSeverity FactorBiorefinery3618564 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/44fa4e0d-d9e4-4cdd-b598-0843916a1ba8/download |
| spellingShingle | Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor Pino, Marcela Sofía Glucan Glucose Biofuels Autohydrolysis Severity Factor Biorefinery |
| status | SINGLETON |
| subject.fl_str_mv | Glucan Glucose Biofuels Autohydrolysis Severity Factor Biorefinery |
| title | Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor |
| title_full | Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor |
| title_fullStr | Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor |
| title_full_unstemmed | Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor |
| title_short | Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor |
| title_sort | Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor |
| topic | Glucan Glucose Biofuels Autohydrolysis Severity Factor Biorefinery |
| topic_facet | Glucan Glucose Biofuels Autohydrolysis Severity Factor Biorefinery |
| url | https://hdl.handle.net/1822/59092 |
| visible | 1 |