Publicação
Antibacterial and antioxidant activities of Streptomyces sp. strain FR7 isolated from forest soil
| Resumo: | Actinomycetes produce secondary metabolites with many bioactivities such as antimicrobial, which can be useful as alternatives against resistant bacterial strains. Therefore, the screening of new habitats is likely to provide new strains with high potential. In this work, the antimicrobial capacity was used to select Streptomyces sp. strains isolated from Raf Raf forest (Tunisia). From the strain displaying higher activity, FR7, an ethyl acetate extract was prepared under optimized culturing conditions (10 days at 30°C in ISP2 medium with initial pH 8), showing significant antimicrobial activity against Micrococcus luteus and Staphylococcus aureus (MIC = 5 μg ml-1), and Listeria monocytogenes and Pseudomonas aeruginosa (MIC = 20 μg ml-1). The extract displayed strong DPPH radical scavenging activity (IC50 = 1.3 μg ml-1) and protection of yeast cells from H2O2-induced oxidative stress determined by flow cytometry with dichlorofluorescein diacetate. The crude extract showed the presence of polyketides, with methylsalicylic acid as moiety, a large and diverse group of secondary metabolites with a wide range of bioactivities, including antioxidant and antibacterial. Based on 16S RNA gene sequences, strain FR7 was identified as belonging to genus Streptomyces with high resemblance to S. iakyrus. Streptomyces sp. FR7 has great potential as a source of antibacterial and antioxidant metabolites. |
|---|---|
| Autores principais: | Weslati, Imen |
| Outros Autores: | Simões, Luara Aparecida; Teixeira, Ana; Parpot, Pier; Raies, Aly; Oliveira, Rui Pedro Soares de |
| Assunto: | Antioxidants Soil Hydrogen Peroxide Microbial Sensitivity Tests Anti-Bacterial Agents Forests Streptomyces Anti-Infective Agents Actinomycetes Streptomycetes Antimicrobials Polyketides Ciências Naturais::Ciências Biológicas |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439685873172480 |
|---|---|
| author | Weslati, Imen |
| author2 | Simões, Luara Aparecida Teixeira, Ana Parpot, Pier Raies, Aly Oliveira, Rui Pedro Soares de |
| author2_role | author author author author author |
| author_facet | Weslati, Imen Simões, Luara Aparecida Teixeira, Ana Parpot, Pier Raies, Aly Oliveira, Rui Pedro Soares de |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Weslati, Imen\"},{\"Person.name\":\"Simões, Luara Aparecida\"},{\"Person.name\":\"Teixeira, Ana\"},{\"Person.name\":\"Parpot, Pier\"},{\"Person.name\":\"Raies, Aly\"},{\"Person.name\":\"Oliveira, Rui Pedro Soares de\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Weslati, Imen Simões, Luara Aparecida Teixeira, Ana Parpot, Pier Raies, Aly Oliveira, Rui Pedro Soares de |
| datacite.date.Accepted.fl_str_mv | 2023-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2024-04-02T13:55:54Z |
| datacite.date.embargoed.fl_str_mv | 2024-04-02T13:55:54Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Antioxidants Soil Hydrogen Peroxide Microbial Sensitivity Tests Anti-Bacterial Agents Forests Streptomyces Anti-Infective Agents Actinomycetes Streptomycetes Antimicrobials Polyketides Ciências Naturais::Ciências Biológicas |
| datacite.titles.title.fl_str_mv | Antibacterial and antioxidant activities of Streptomyces sp. strain FR7 isolated from forest soil |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Weslati, Imen Simões, Luara Aparecida Teixeira, Ana Parpot, Pier Raies, Aly Oliveira, Rui Pedro Soares de |
| dc.date.Accepted.fl_str_mv | 2023-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2024-04-02T13:55:54Z |
| dc.date.embargoed.fl_str_mv | 2024-04-02T13:55:54Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/90384 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Oxford University Press |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Antioxidants Soil Hydrogen Peroxide Microbial Sensitivity Tests Anti-Bacterial Agents Forests Streptomyces Anti-Infective Agents Actinomycetes Streptomycetes Antimicrobials Polyketides Ciências Naturais::Ciências Biológicas |
| dc.title.fl_str_mv | Antibacterial and antioxidant activities of Streptomyces sp. strain FR7 isolated from forest soil |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Actinomycetes produce secondary metabolites with many bioactivities such as antimicrobial, which can be useful as alternatives against resistant bacterial strains. Therefore, the screening of new habitats is likely to provide new strains with high potential. In this work, the antimicrobial capacity was used to select Streptomyces sp. strains isolated from Raf Raf forest (Tunisia). From the strain displaying higher activity, FR7, an ethyl acetate extract was prepared under optimized culturing conditions (10 days at 30°C in ISP2 medium with initial pH 8), showing significant antimicrobial activity against Micrococcus luteus and Staphylococcus aureus (MIC = 5 μg ml-1), and Listeria monocytogenes and Pseudomonas aeruginosa (MIC = 20 μg ml-1). The extract displayed strong DPPH radical scavenging activity (IC50 = 1.3 μg ml-1) and protection of yeast cells from H2O2-induced oxidative stress determined by flow cytometry with dichlorofluorescein diacetate. The crude extract showed the presence of polyketides, with methylsalicylic acid as moiety, a large and diverse group of secondary metabolites with a wide range of bioactivities, including antioxidant and antibacterial. Based on 16S RNA gene sequences, strain FR7 was identified as belonging to genus Streptomyces with high resemblance to S. iakyrus. Streptomyces sp. FR7 has great potential as a source of antibacterial and antioxidant metabolites. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/e5040231-90c8-4502-9cd4-7900fefde568/download |
| id | rum_9e4e1dc4f3baf94a4eece906bbf95f0a |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/90384 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/90384 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Weslati, Imen Simões, Luara Aparecida Teixeira, Ana Parpot, Pier Raies, Aly Oliveira, Rui Pedro Soares de |
| publishDate | 2023 |
| publisher.none.fl_str_mv | Oxford University Press |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engOxford University PressporActinomycetes produce secondary metabolites with many bioactivities such as antimicrobial, which can be useful as alternatives against resistant bacterial strains. Therefore, the screening of new habitats is likely to provide new strains with high potential. In this work, the antimicrobial capacity was used to select Streptomyces sp. strains isolated from Raf Raf forest (Tunisia). From the strain displaying higher activity, FR7, an ethyl acetate extract was prepared under optimized culturing conditions (10 days at 30°C in ISP2 medium with initial pH 8), showing significant antimicrobial activity against Micrococcus luteus and Staphylococcus aureus (MIC = 5 μg ml-1), and Listeria monocytogenes and Pseudomonas aeruginosa (MIC = 20 μg ml-1). The extract displayed strong DPPH radical scavenging activity (IC50 = 1.3 μg ml-1) and protection of yeast cells from H2O2-induced oxidative stress determined by flow cytometry with dichlorofluorescein diacetate. The crude extract showed the presence of polyketides, with methylsalicylic acid as moiety, a large and diverse group of secondary metabolites with a wide range of bioactivities, including antioxidant and antibacterial. Based on 16S RNA gene sequences, strain FR7 was identified as belonging to genus Streptomyces with high resemblance to S. iakyrus. Streptomyces sp. FR7 has great potential as a source of antibacterial and antioxidant metabolites.application/pdfporAntibacterial and antioxidant activities of Streptomyces sp. strain FR7 isolated from forest soilWeslati, ImenSimões, Luara AparecidaTeixeira, AnaParpot, PierRaies, AlyOliveira, Rui Pedro Soares deHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONWeslati, I., Simões, L., Teixeira, A., Parpot, P., Raies, A., & Oliveira, R. (2023, March 16). Antibacterial and antioxidant activities of Streptomyces sp. strain FR7 isolated from forest soil. Letters in Applied Microbiology. Oxford University Press (OUP). http://doi.org/10.1093/lambio/ovad036PMID36928103ISSNIsPartOf0266-8254DOIIsPartOf10.1093/lambio/ovad0362024-04-02T13:55:54Z20232023-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/90384http://purl.org/coar/access_right/c_abf2open accessAntioxidantsSoilHydrogen PeroxideMicrobial Sensitivity TestsAnti-Bacterial AgentsForestsStreptomycesAnti-Infective AgentsActinomycetesStreptomycetesAntimicrobialsPolyketideshttp://www.oecd.org/science/inno/38235147.pdfFields of Science and Technology (FOS)Ciências Naturais::Ciências Biológicas458361 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/e5040231-90c8-4502-9cd4-7900fefde568/download |
| spellingShingle | Antibacterial and antioxidant activities of Streptomyces sp. strain FR7 isolated from forest soil Weslati, Imen Antioxidants Soil Hydrogen Peroxide Microbial Sensitivity Tests Anti-Bacterial Agents Forests Streptomyces Anti-Infective Agents Actinomycetes Streptomycetes Antimicrobials Polyketides Ciências Naturais::Ciências Biológicas |
| status | SINGLETON |
| subject.fl_str_mv | Antioxidants Soil Hydrogen Peroxide Microbial Sensitivity Tests Anti-Bacterial Agents Forests Streptomyces Anti-Infective Agents Actinomycetes Streptomycetes Antimicrobials Polyketides |
| subject.other.fl_str_mv | Ciências Naturais::Ciências Biológicas |
| title | Antibacterial and antioxidant activities of Streptomyces sp. strain FR7 isolated from forest soil |
| title_full | Antibacterial and antioxidant activities of Streptomyces sp. strain FR7 isolated from forest soil |
| title_fullStr | Antibacterial and antioxidant activities of Streptomyces sp. strain FR7 isolated from forest soil |
| title_full_unstemmed | Antibacterial and antioxidant activities of Streptomyces sp. strain FR7 isolated from forest soil |
| title_short | Antibacterial and antioxidant activities of Streptomyces sp. strain FR7 isolated from forest soil |
| title_sort | Antibacterial and antioxidant activities of Streptomyces sp. strain FR7 isolated from forest soil |
| topic | Antioxidants Soil Hydrogen Peroxide Microbial Sensitivity Tests Anti-Bacterial Agents Forests Streptomyces Anti-Infective Agents Actinomycetes Streptomycetes Antimicrobials Polyketides Ciências Naturais::Ciências Biológicas |
| topic_facet | Antioxidants Soil Hydrogen Peroxide Microbial Sensitivity Tests Anti-Bacterial Agents Forests Streptomyces Anti-Infective Agents Actinomycetes Streptomycetes Antimicrobials Polyketides Ciências Naturais::Ciências Biológicas |
| url | https://hdl.handle.net/1822/90384 |
| visible | 1 |