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Maximization of fructooligosaccharides and β-fructofuranosidase production by Aspergillus japonicus under solid-state fermentation conditions

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Resumo:The conditions of temperature, moisture content, and inoculum rate able to maximize the production of fructooligosaccharides (FOS) and β-fructofuranosidase (FFase) enzyme by solid-state fermentation were established. Fermentation assays were performed using the support material (coffee silverskin) moistened to 60, 70, or 80 % with a 240-g/l sucrose solution and inoculated with a spore suspension of Aspergillus japonicus to obtain 2 × 10 5, 2 × 10 6, or 2 × 10 7 spores/gram dry material. The fermentation runs were maintained under static conditions at 26, 30, or 34 °C during 20 h. The moisture content did not influence the FOS and FFase production; however, temperature between 26 and 30 °C and inoculum rate of approximately 2 × 10 7 spores/gram dry material maximized the results (FOS = 208.8 g/l with productivity of 10.44 g/l h; FFase = 64.12 units U/ml with productivity of 4.0 U/ml h). These results are considerably higher than those obtained under no optimized fermentation conditions and represent an important contribution for the establishment of a new industrial process for FOS and FFase production.
Autores principais:Mussatto, Solange I.
Outros Autores:Ballesteros, Lina F.; Martins, Silvia; Maltos, Dulce A .F.; Aguilar, Cristóbal N.; Teixeira, J. A.
Assunto:Coffee silverskin Solid-state fermentation Fructooligosaccharides β-Fructofuranosidase Aspergillus japonicus beta-Fructofuranosidase
Ano:2013
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho

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