Publicação
Development, characterization, antioxidant and hepatoprotective properties of poly(Ɛ-caprolactone) nanoparticles loaded with a neuroprotective fraction of Hypericum perforatum
| Resumo: | Quercetin and biapigenin are antioxidant and neuroprotective compounds present in Hypericum perforatum, with potential application in neurodegenerative diseases. Quercetin has shown to have excellent antioxidant activities. Biapigenin possesses a distinctive mechanism of action, preventing the onset of calcium deregulation and mitochondrial dysfunction. The main aim of this study was to establish a new delivery system encapsulating H. perforatum neuroprotective fraction, isolated from it,containing quercetin and biapigenin into poly(Ɛ-caprolactone) (PCL) nanoparticles. It also aimed to study its hepatoprotective potential. Different formulation parameters were optimized, resulting in a stable formulation of polymer:compounds ratio 1:0.1 with a mean particle size of 185nm, zeta potential around -20mV and association efficiency close to 100%. Compounds in vitro release, under physiological conditions, showed an initial burst followed by a sustained release. The antioxidant functional properties of these compounds were not altered by encapsulation. PCL-loaded nanoparticles protected HepG2 cells from intrinsic compound toxicity at high concentrations. Depending on the incubation regimen, quercetin-biapigenin PCL-loaded nanoparticles or free compounds were more effective in protecting HepG2 cells against tert-butylhydroperoxide-induced toxicity. This is the first report of the encapsulation of a quercetin-biapigenin mixture in a polymeric matrix, specifically, in PCL, with synergic anti-oxidant and hepatoprotective effects. |
|---|---|
| Autores principais: | Oliveira, Ana Isabel |
| Outros Autores: | Pinho, Cláudia; Fonte, Pedro; Sarmento, Bruno; Dias, Alberto Carlos Pires |
| Assunto: | Hep G2 Cells Humans Hypericum perforatum Antioxidants Drug Carriers Nanoparticles Neuroprotective Agents Polyesters Quercetin Biapigenin Hepatoprotective In vitro release Anti-oxidant Anti-oxidant |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866877395339837440 |
|---|---|
| author | Oliveira, Ana Isabel |
| author2 | Pinho, Cláudia Fonte, Pedro Sarmento, Bruno Dias, Alberto Carlos Pires |
| author2_role | author author author author |
| author_facet | Oliveira, Ana Isabel Pinho, Cláudia Fonte, Pedro Sarmento, Bruno Dias, Alberto Carlos Pires |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Oliveira, Ana Isabel\"},{\"Person.name\":\"Pinho, Cláudia\"},{\"Person.name\":\"Fonte, Pedro\"},{\"Person.name\":\"Sarmento, Bruno\"},{\"Person.name\":\"Dias, Alberto Carlos Pires\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Oliveira, Ana Isabel Pinho, Cláudia Fonte, Pedro Sarmento, Bruno Dias, Alberto Carlos Pires |
| datacite.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-06-16T09:47:54Z |
| datacite.date.embargoed.fl_str_mv | 2021-06-16T09:47:54Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Hep G2 Cells Humans Hypericum perforatum Antioxidants Drug Carriers Nanoparticles Neuroprotective Agents Polyesters Quercetin Biapigenin Hepatoprotective In vitro release Anti-oxidant Anti-oxidant |
| datacite.titles.title.fl_str_mv | Development, characterization, antioxidant and hepatoprotective properties of poly(Ɛ-caprolactone) nanoparticles loaded with a neuroprotective fraction of Hypericum perforatum |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Oliveira, Ana Isabel Pinho, Cláudia Fonte, Pedro Sarmento, Bruno Dias, Alberto Carlos Pires |
| dc.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-06-16T09:47:54Z |
| dc.date.embargoed.fl_str_mv | 2021-06-16T09:47:54Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/73411 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Hep G2 Cells Humans Hypericum perforatum Antioxidants Drug Carriers Nanoparticles Neuroprotective Agents Polyesters Quercetin Biapigenin Hepatoprotective In vitro release Anti-oxidant Anti-oxidant |
| dc.title.fl_str_mv | Development, characterization, antioxidant and hepatoprotective properties of poly(Ɛ-caprolactone) nanoparticles loaded with a neuroprotective fraction of Hypericum perforatum |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Quercetin and biapigenin are antioxidant and neuroprotective compounds present in Hypericum perforatum, with potential application in neurodegenerative diseases. Quercetin has shown to have excellent antioxidant activities. Biapigenin possesses a distinctive mechanism of action, preventing the onset of calcium deregulation and mitochondrial dysfunction. The main aim of this study was to establish a new delivery system encapsulating H. perforatum neuroprotective fraction, isolated from it,containing quercetin and biapigenin into poly(Ɛ-caprolactone) (PCL) nanoparticles. It also aimed to study its hepatoprotective potential. Different formulation parameters were optimized, resulting in a stable formulation of polymer:compounds ratio 1:0.1 with a mean particle size of 185nm, zeta potential around -20mV and association efficiency close to 100%. Compounds in vitro release, under physiological conditions, showed an initial burst followed by a sustained release. The antioxidant functional properties of these compounds were not altered by encapsulation. PCL-loaded nanoparticles protected HepG2 cells from intrinsic compound toxicity at high concentrations. Depending on the incubation regimen, quercetin-biapigenin PCL-loaded nanoparticles or free compounds were more effective in protecting HepG2 cells against tert-butylhydroperoxide-induced toxicity. This is the first report of the encapsulation of a quercetin-biapigenin mixture in a polymeric matrix, specifically, in PCL, with synergic anti-oxidant and hepatoprotective effects. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/b30aaeda-7781-4279-848d-f36f4f7b0de4/download |
| id | rum_a3cdcbfb779fdec07e8c390751d87f64 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/73411 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/73411 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Oliveira, Ana Isabel Pinho, Cláudia Fonte, Pedro Sarmento, Bruno Dias, Alberto Carlos Pires |
| publishDate | 2018 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engElsevierporQuercetin and biapigenin are antioxidant and neuroprotective compounds present in Hypericum perforatum, with potential application in neurodegenerative diseases. Quercetin has shown to have excellent antioxidant activities. Biapigenin possesses a distinctive mechanism of action, preventing the onset of calcium deregulation and mitochondrial dysfunction. The main aim of this study was to establish a new delivery system encapsulating H. perforatum neuroprotective fraction, isolated from it,containing quercetin and biapigenin into poly(Ɛ-caprolactone) (PCL) nanoparticles. It also aimed to study its hepatoprotective potential. Different formulation parameters were optimized, resulting in a stable formulation of polymer:compounds ratio 1:0.1 with a mean particle size of 185nm, zeta potential around -20mV and association efficiency close to 100%. Compounds in vitro release, under physiological conditions, showed an initial burst followed by a sustained release. The antioxidant functional properties of these compounds were not altered by encapsulation. PCL-loaded nanoparticles protected HepG2 cells from intrinsic compound toxicity at high concentrations. Depending on the incubation regimen, quercetin-biapigenin PCL-loaded nanoparticles or free compounds were more effective in protecting HepG2 cells against tert-butylhydroperoxide-induced toxicity. This is the first report of the encapsulation of a quercetin-biapigenin mixture in a polymeric matrix, specifically, in PCL, with synergic anti-oxidant and hepatoprotective effects.application/pdfporDevelopment, characterization, antioxidant and hepatoprotective properties of poly(Ɛ-caprolactone) nanoparticles loaded with a neuroprotective fraction of Hypericum perforatumOliveira, Ana IsabelPinho, CláudiaFonte, PedroSarmento, BrunoDias, Alberto Carlos PiresHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf0141-8130DOIIsPartOf10.1016/j.ijbiomac.2017.10.1032021-06-16T09:47:54Z20182018-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/73411http://purl.org/coar/access_right/c_abf2open accessHep G2 CellsHumansHypericum perforatumAntioxidantsDrug CarriersNanoparticlesNeuroprotective AgentsPolyestersQuercetinBiapigeninHepatoprotectiveIn vitro releaseAnti-oxidantAnti-oxidant2301241 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/b30aaeda-7781-4279-848d-f36f4f7b0de4/download |
| spellingShingle | Development, characterization, antioxidant and hepatoprotective properties of poly(Ɛ-caprolactone) nanoparticles loaded with a neuroprotective fraction of Hypericum perforatum Oliveira, Ana Isabel Hep G2 Cells Humans Hypericum perforatum Antioxidants Drug Carriers Nanoparticles Neuroprotective Agents Polyesters Quercetin Biapigenin Hepatoprotective In vitro release Anti-oxidant Anti-oxidant |
| status | SINGLETON |
| subject.fl_str_mv | Hep G2 Cells Humans Hypericum perforatum Antioxidants Drug Carriers Nanoparticles Neuroprotective Agents Polyesters Quercetin Biapigenin Hepatoprotective In vitro release Anti-oxidant Anti-oxidant |
| title | Development, characterization, antioxidant and hepatoprotective properties of poly(Ɛ-caprolactone) nanoparticles loaded with a neuroprotective fraction of Hypericum perforatum |
| title_full | Development, characterization, antioxidant and hepatoprotective properties of poly(Ɛ-caprolactone) nanoparticles loaded with a neuroprotective fraction of Hypericum perforatum |
| title_fullStr | Development, characterization, antioxidant and hepatoprotective properties of poly(Ɛ-caprolactone) nanoparticles loaded with a neuroprotective fraction of Hypericum perforatum |
| title_full_unstemmed | Development, characterization, antioxidant and hepatoprotective properties of poly(Ɛ-caprolactone) nanoparticles loaded with a neuroprotective fraction of Hypericum perforatum |
| title_short | Development, characterization, antioxidant and hepatoprotective properties of poly(Ɛ-caprolactone) nanoparticles loaded with a neuroprotective fraction of Hypericum perforatum |
| title_sort | Development, characterization, antioxidant and hepatoprotective properties of poly(Ɛ-caprolactone) nanoparticles loaded with a neuroprotective fraction of Hypericum perforatum |
| topic | Hep G2 Cells Humans Hypericum perforatum Antioxidants Drug Carriers Nanoparticles Neuroprotective Agents Polyesters Quercetin Biapigenin Hepatoprotective In vitro release Anti-oxidant Anti-oxidant |
| topic_facet | Hep G2 Cells Humans Hypericum perforatum Antioxidants Drug Carriers Nanoparticles Neuroprotective Agents Polyesters Quercetin Biapigenin Hepatoprotective In vitro release Anti-oxidant Anti-oxidant |
| url | https://hdl.handle.net/1822/73411 |
| visible | 1 |