Publicação
Antimicrobial enzyme immobilization in bacterial cellulose
| Resumo: | This work studied the physical immobilization of laccase on bacterial nanocellulose (BNC) aiming to identify the enzyme antibacterial properties suitable for wound dressings. The pH optimum and activation energy of free laccase depends on the substrate employed. The Michaelis-Menten constant for the immobilized laccase was found to be almost double of that of the free enzyme. However, the specific activities of immobilized and free laccase are similar suggesting that entrapped laccase on BNC maintain some flexibility and favour substrate accessibility. The results clearly show the antimicrobial effect of laccase and cytotoxicity acceptable for wound dressing applications. |
|---|---|
| Autores principais: | Sampaio, Liliana M. P. |
| Outros Autores: | Ribeiro, Jorge Manuel Padrão; Faria, Jorge; Silva, João Pedro Martins Soares Castro; Silva, Carla J.; Dourado, Fernando; Zille, Andrea |
| Assunto: | Bacterial nanocellulose Laccase Antimicrobial Immobilization |
| Ano: | 2017 |
| País: | Portugal |
| Tipo de documento: | outro |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866876773393760256 |
|---|---|
| author | Sampaio, Liliana M. P. |
| author2 | Ribeiro, Jorge Manuel Padrão Faria, Jorge Silva, João Pedro Martins Soares Castro Silva, Carla J. Dourado, Fernando Zille, Andrea |
| author2_role | author author author author author author |
| author_facet | Sampaio, Liliana M. P. Ribeiro, Jorge Manuel Padrão Faria, Jorge Silva, João Pedro Martins Soares Castro Silva, Carla J. Dourado, Fernando Zille, Andrea |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Sampaio, Liliana M. P.\"},{\"Person.name\":\"Ribeiro, Jorge Manuel Padrão\"},{\"Person.name\":\"Faria, Jorge\"},{\"Person.name\":\"Silva, João Pedro Martins Soares Castro\"},{\"Person.name\":\"Silva, Carla J.\"},{\"Person.name\":\"Dourado, Fernando\"},{\"Person.name\":\"Zille, Andrea\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Sampaio, Liliana M. P. Ribeiro, Jorge Manuel Padrão Faria, Jorge Silva, João Pedro Martins Soares Castro Silva, Carla J. Dourado, Fernando Zille, Andrea |
| datacite.date.Accepted.fl_str_mv | 2017-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2017-06-28T12:40:31Z |
| datacite.date.embargoed.fl_str_mv | 2017-06-28T12:40:31Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Bacterial nanocellulose Laccase Antimicrobial Immobilization |
| datacite.titles.title.fl_str_mv | Antimicrobial enzyme immobilization in bacterial cellulose |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Sampaio, Liliana M. P. Ribeiro, Jorge Manuel Padrão Faria, Jorge Silva, João Pedro Martins Soares Castro Silva, Carla J. Dourado, Fernando Zille, Andrea |
| dc.date.Accepted.fl_str_mv | 2017-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2017-06-28T12:40:31Z |
| dc.date.embargoed.fl_str_mv | 2017-06-28T12:40:31Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/46083 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Bacterial nanocellulose Laccase Antimicrobial Immobilization |
| dc.title.fl_str_mv | Antimicrobial enzyme immobilization in bacterial cellulose |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_1843 |
| description | This work studied the physical immobilization of laccase on bacterial nanocellulose (BNC) aiming to identify the enzyme antibacterial properties suitable for wound dressings. The pH optimum and activation energy of free laccase depends on the substrate employed. The Michaelis-Menten constant for the immobilized laccase was found to be almost double of that of the free enzyme. However, the specific activities of immobilized and free laccase are similar suggesting that entrapped laccase on BNC maintain some flexibility and favour substrate accessibility. The results clearly show the antimicrobial effect of laccase and cytotoxicity acceptable for wound dressing applications. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | other |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/d97917b2-6c99-4d02-81e5-cad16056e8aa/download |
| id | rum_a9eb51f7c41aa6f7d01be21bdfbb78af |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/46083 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/46083 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Sampaio, Liliana M. P. Ribeiro, Jorge Manuel Padrão Faria, Jorge Silva, João Pedro Martins Soares Castro Silva, Carla J. Dourado, Fernando Zille, Andrea |
| publishDate | 2017 |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engporThis work studied the physical immobilization of laccase on bacterial nanocellulose (BNC) aiming to identify the enzyme antibacterial properties suitable for wound dressings. The pH optimum and activation energy of free laccase depends on the substrate employed. The Michaelis-Menten constant for the immobilized laccase was found to be almost double of that of the free enzyme. However, the specific activities of immobilized and free laccase are similar suggesting that entrapped laccase on BNC maintain some flexibility and favour substrate accessibility. The results clearly show the antimicrobial effect of laccase and cytotoxicity acceptable for wound dressing applications.application/pdfporAntimicrobial enzyme immobilization in bacterial celluloseSampaio, Liliana M. P.Ribeiro, Jorge Manuel PadrãoFaria, JorgeSilva, João Pedro Martins Soares CastroSilva, Carla J.Dourado, FernandoZille, AndreaHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.pt2017-06-28T12:40:31Z20172017-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/46083http://purl.org/coar/access_right/c_abf2open accessBacterial nanocelluloseLaccaseAntimicrobialImmobilization553961 bytesother research producthttp://purl.org/coar/resource_type/c_1843otherhttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/d97917b2-6c99-4d02-81e5-cad16056e8aa/download |
| spellingShingle | Antimicrobial enzyme immobilization in bacterial cellulose Sampaio, Liliana M. P. Bacterial nanocellulose Laccase Antimicrobial Immobilization |
| status | SINGLETON |
| subject.fl_str_mv | Bacterial nanocellulose Laccase Antimicrobial Immobilization |
| title | Antimicrobial enzyme immobilization in bacterial cellulose |
| title_full | Antimicrobial enzyme immobilization in bacterial cellulose |
| title_fullStr | Antimicrobial enzyme immobilization in bacterial cellulose |
| title_full_unstemmed | Antimicrobial enzyme immobilization in bacterial cellulose |
| title_short | Antimicrobial enzyme immobilization in bacterial cellulose |
| title_sort | Antimicrobial enzyme immobilization in bacterial cellulose |
| topic | Bacterial nanocellulose Laccase Antimicrobial Immobilization |
| topic_facet | Bacterial nanocellulose Laccase Antimicrobial Immobilization |
| url | https://hdl.handle.net/1822/46083 |
| visible | 1 |