Publicação
A new model of laryngitis: neuropeptide, cyclooxygenase, and cytokine profile
| Resumo: | Objectives/Hypothesis: To develop and characterize a new model of laryngeal inflammation by analyzing the presence of neurogenic peptides and expression of cyclooxygenases (COX) and cytokines in the mucosa.Study Design: Laryngitis induced by nasogastric intubation (NGI) was evaluated by histopathologic changes of the mucosa, alterations in calcitonin gene related peptide (CGRP) and substance P (SP) neuropeptides in sensory fibers, and COX-1,2, and cytokine (interleukin [IL]-1, IL-6, IL-10, tumor necrosis factor [TNF]-alpha) expression in the laryngeal mucosa.Methods: Rats submitted to NGI for 1 to 5 weeks were compared with controls. Laryngeal sections were immunostained for stereologic analysis of SP and CGRP fiber density and number of mucosal cells expressing COX-2. Alterations in inflammatory mediators were evaluated by quantitative reverse-transcriptase polymerase chain reaction.Results: NGI induced metaplasia of the epithelium and narrowing of the laryngeal lumen because of hypertrophy of laryngeal glandules and edema. An initial decrease in CGRP- and SP-immunoreactive fibers in the laryngeal mucosa (1-3 wk) was reverted with time (5 wk). COX-2 expression in mucosal cells increased progressively, reaching a maximum level at 5 weeks, and was observed in mononuclear immune cells, which is indicative of a chronic inflammatory process. In regard to mRNA expression levels of inflammatory mediators, TNF-alpha was increased during the 5 week NGI, and IL-10 decreased during the 5 weeks,whereas IL-beta, IL-6, and COX-2 increased in the first 1 to 2 weeks and returned to baseline at 5 weeks.Conclusions: This NGI model results in laryngeal chronic inflammation without direct mechanical aggression of the mucosa and may contribute to the study of future therapeutic approaches to this pathology. |
|---|---|
| Autores principais: | Rodrigues, Manuel Lima |
| Outros Autores: | Fernandes, Ana Valle; Lamas, Nuno Jorge; Cruz, Andrea; Baltazar, Fátima; Milanezi, Fernanda; Nunes, Rui; Reis, R. M.; Pedrosa, Jorge; Castro, António G.; Almeida, Armando |
| Assunto: | Laryngeal inflammation Neuropeptides Cyclooxygenase-2 Cytokines Nasogastric intubation Immunocytochemistry MRNA expression Reverse transcriptase polymerase chain reaction analysis Animal model |
| Ano: | 2008 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866876614252429312 |
|---|---|
| author | Rodrigues, Manuel Lima |
| author2 | Fernandes, Ana Valle Lamas, Nuno Jorge Cruz, Andrea Baltazar, Fátima Milanezi, Fernanda Nunes, Rui Reis, R. M. Pedrosa, Jorge Castro, António G. Almeida, Armando |
| author2_role | author author author author author author author author author author |
| author_facet | Rodrigues, Manuel Lima Fernandes, Ana Valle Lamas, Nuno Jorge Cruz, Andrea Baltazar, Fátima Milanezi, Fernanda Nunes, Rui Reis, R. M. Pedrosa, Jorge Castro, António G. Almeida, Armando |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Rodrigues, Manuel Lima\"},{\"Person.name\":\"Fernandes, Ana Valle\"},{\"Person.name\":\"Lamas, Nuno Jorge\"},{\"Person.name\":\"Cruz, Andrea\"},{\"Person.name\":\"Baltazar, Fátima\"},{\"Person.name\":\"Milanezi, Fernanda\"},{\"Person.name\":\"Nunes, Rui\"},{\"Person.name\":\"Reis, R. M.\"},{\"Person.name\":\"Pedrosa, Jorge\"},{\"Person.name\":\"Castro, António G.\"},{\"Person.name\":\"Almeida, Armando\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Rodrigues, Manuel Lima Fernandes, Ana Valle Lamas, Nuno Jorge Cruz, Andrea Baltazar, Fátima Milanezi, Fernanda Nunes, Rui Reis, R. M. Pedrosa, Jorge Castro, António G. Almeida, Armando |
| datacite.date.Accepted.fl_str_mv | 2008-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2008-06-03T14:42:22Z |
| datacite.date.embargoed.fl_str_mv | 2008-06-03T14:42:22Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Laryngeal inflammation Neuropeptides Cyclooxygenase-2 Cytokines Nasogastric intubation Immunocytochemistry MRNA expression Reverse transcriptase polymerase chain reaction analysis Animal model |
| datacite.titles.title.fl_str_mv | A new model of laryngitis: neuropeptide, cyclooxygenase, and cytokine profile |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Rodrigues, Manuel Lima Fernandes, Ana Valle Lamas, Nuno Jorge Cruz, Andrea Baltazar, Fátima Milanezi, Fernanda Nunes, Rui Reis, R. M. Pedrosa, Jorge Castro, António G. Almeida, Armando |
| dc.date.Accepted.fl_str_mv | 2008-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2008-06-03T14:42:22Z |
| dc.date.embargoed.fl_str_mv | 2008-06-03T14:42:22Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/7897 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Lippincott, Williams & Wilkins |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Laryngeal inflammation Neuropeptides Cyclooxygenase-2 Cytokines Nasogastric intubation Immunocytochemistry MRNA expression Reverse transcriptase polymerase chain reaction analysis Animal model |
| dc.title.fl_str_mv | A new model of laryngitis: neuropeptide, cyclooxygenase, and cytokine profile |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Objectives/Hypothesis: To develop and characterize a new model of laryngeal inflammation by analyzing the presence of neurogenic peptides and expression of cyclooxygenases (COX) and cytokines in the mucosa.Study Design: Laryngitis induced by nasogastric intubation (NGI) was evaluated by histopathologic changes of the mucosa, alterations in calcitonin gene related peptide (CGRP) and substance P (SP) neuropeptides in sensory fibers, and COX-1,2, and cytokine (interleukin [IL]-1, IL-6, IL-10, tumor necrosis factor [TNF]-alpha) expression in the laryngeal mucosa.Methods: Rats submitted to NGI for 1 to 5 weeks were compared with controls. Laryngeal sections were immunostained for stereologic analysis of SP and CGRP fiber density and number of mucosal cells expressing COX-2. Alterations in inflammatory mediators were evaluated by quantitative reverse-transcriptase polymerase chain reaction.Results: NGI induced metaplasia of the epithelium and narrowing of the laryngeal lumen because of hypertrophy of laryngeal glandules and edema. An initial decrease in CGRP- and SP-immunoreactive fibers in the laryngeal mucosa (1-3 wk) was reverted with time (5 wk). COX-2 expression in mucosal cells increased progressively, reaching a maximum level at 5 weeks, and was observed in mononuclear immune cells, which is indicative of a chronic inflammatory process. In regard to mRNA expression levels of inflammatory mediators, TNF-alpha was increased during the 5 week NGI, and IL-10 decreased during the 5 weeks,whereas IL-beta, IL-6, and COX-2 increased in the first 1 to 2 weeks and returned to baseline at 5 weeks.Conclusions: This NGI model results in laryngeal chronic inflammation without direct mechanical aggression of the mucosa and may contribute to the study of future therapeutic approaches to this pathology. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/45664afb-34f2-4d9f-9d66-3ddf7e99e4b2/download |
| id | rum_ad263c1bcb11e6ba61347203ee05fa4e |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/7897 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/7897 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Rodrigues, Manuel Lima Fernandes, Ana Valle Lamas, Nuno Jorge Cruz, Andrea Baltazar, Fátima Milanezi, Fernanda Nunes, Rui Reis, R. M. Pedrosa, Jorge Castro, António G. Almeida, Armando |
| publishDate | 2008 |
| publisher.none.fl_str_mv | Lippincott, Williams & Wilkins |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engLippincott, Williams & WilkinsporObjectives/Hypothesis: To develop and characterize a new model of laryngeal inflammation by analyzing the presence of neurogenic peptides and expression of cyclooxygenases (COX) and cytokines in the mucosa.Study Design: Laryngitis induced by nasogastric intubation (NGI) was evaluated by histopathologic changes of the mucosa, alterations in calcitonin gene related peptide (CGRP) and substance P (SP) neuropeptides in sensory fibers, and COX-1,2, and cytokine (interleukin [IL]-1, IL-6, IL-10, tumor necrosis factor [TNF]-alpha) expression in the laryngeal mucosa.Methods: Rats submitted to NGI for 1 to 5 weeks were compared with controls. Laryngeal sections were immunostained for stereologic analysis of SP and CGRP fiber density and number of mucosal cells expressing COX-2. Alterations in inflammatory mediators were evaluated by quantitative reverse-transcriptase polymerase chain reaction.Results: NGI induced metaplasia of the epithelium and narrowing of the laryngeal lumen because of hypertrophy of laryngeal glandules and edema. An initial decrease in CGRP- and SP-immunoreactive fibers in the laryngeal mucosa (1-3 wk) was reverted with time (5 wk). COX-2 expression in mucosal cells increased progressively, reaching a maximum level at 5 weeks, and was observed in mononuclear immune cells, which is indicative of a chronic inflammatory process. In regard to mRNA expression levels of inflammatory mediators, TNF-alpha was increased during the 5 week NGI, and IL-10 decreased during the 5 weeks,whereas IL-beta, IL-6, and COX-2 increased in the first 1 to 2 weeks and returned to baseline at 5 weeks.Conclusions: This NGI model results in laryngeal chronic inflammation without direct mechanical aggression of the mucosa and may contribute to the study of future therapeutic approaches to this pathology.application/pdfengA new model of laryngitis: neuropeptide, cyclooxygenase, and cytokine profileRodrigues, Manuel LimaFernandes, Ana ValleLamas, Nuno JorgeCruz, AndreaBaltazar, FátimaMilanezi, FernandaNunes, RuiReis, R. M.Pedrosa, JorgeCastro, António G.Almeida, ArmandoHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf0023-852XDOIIsPartOf10.1097/MLG.0b013e31814924002008-06-03T14:42:22Z2008-012007-06-272008-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/7897http://purl.org/coar/access_right/c_abf2open accessLaryngeal inflammationNeuropeptidesCyclooxygenase-2CytokinesNasogastric intubationImmunocytochemistryMRNA expressionReverse transcriptase polymerase chain reaction analysisAnimal model1584215 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/45664afb-34f2-4d9f-9d66-3ddf7e99e4b2/download |
| spellingShingle | A new model of laryngitis: neuropeptide, cyclooxygenase, and cytokine profile Rodrigues, Manuel Lima Laryngeal inflammation Neuropeptides Cyclooxygenase-2 Cytokines Nasogastric intubation Immunocytochemistry MRNA expression Reverse transcriptase polymerase chain reaction analysis Animal model |
| status | SINGLETON |
| subject.fl_str_mv | Laryngeal inflammation Neuropeptides Cyclooxygenase-2 Cytokines Nasogastric intubation Immunocytochemistry MRNA expression Reverse transcriptase polymerase chain reaction analysis Animal model |
| title | A new model of laryngitis: neuropeptide, cyclooxygenase, and cytokine profile |
| title_full | A new model of laryngitis: neuropeptide, cyclooxygenase, and cytokine profile |
| title_fullStr | A new model of laryngitis: neuropeptide, cyclooxygenase, and cytokine profile |
| title_full_unstemmed | A new model of laryngitis: neuropeptide, cyclooxygenase, and cytokine profile |
| title_short | A new model of laryngitis: neuropeptide, cyclooxygenase, and cytokine profile |
| title_sort | A new model of laryngitis: neuropeptide, cyclooxygenase, and cytokine profile |
| topic | Laryngeal inflammation Neuropeptides Cyclooxygenase-2 Cytokines Nasogastric intubation Immunocytochemistry MRNA expression Reverse transcriptase polymerase chain reaction analysis Animal model |
| topic_facet | Laryngeal inflammation Neuropeptides Cyclooxygenase-2 Cytokines Nasogastric intubation Immunocytochemistry MRNA expression Reverse transcriptase polymerase chain reaction analysis Animal model |
| url | https://hdl.handle.net/1822/7897 |
| visible | 1 |