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Forecast cancer: the importance of biomimetic 3D in vitro models in cancer drug testing/discovery and therapy

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Resumo:Nowadays, the pharmaceutical industry faces a significant challenge related to the high attrition rates of drugs, which is particularly astonishing in oncology [1]. Indeed, several studies have reported that about 95% of anti-cancer drugs tested in phase I fail to reach the market. The reasons for this decline may be very complex, but most likely, they are related to how potential drug candidates are tested, which is far from being optimal. Typically, drugs are evaluated using cancer cells seeded on conventionalâ flatâ tissue culture surfaces (e.g., Petri dishes, flasks, or multi-well plates), which cannot recapitulate the three-dimensional (3D) architecture, rich cellular content, and complex interactions of the native tumor microenvironment [2]. Under these oversimplistic conditions, cancer cells display extreme phenotypes and aberrant gene expression, producing a non-physiological response when exposed to drugs. Additionally, these conventional approaches cannot recapitulate many of the dynamic events occurring in the human body that are crucial in cancer dissemination, particularly fluid flow or gradient formation [3, 4]. Similarly, animal models (e.g., mice, pigs, or primates), besides being ethically controversial and expensive, do not mimic human physiology, particularly the immune system, which is critical during cancer progression and therapy response. Even though â humanizedâ mice have been reported to address this limitation, they are not available for the majority of research labs and are incompatible with high-throughput production, an imperative drug discovery/screening feature.
Autores principais:Caballero, David
Outros Autores:Kundu, B.; Abreu, Catarina M.; Amorim, S.; Fernandes, D. C.; Pires, R. A.; Oliveira, Joaquim M.; Correlo, V. M.; Reis, R. L.; Kundu, Subhas C
Assunto:3D in vitro models Cancer drug screening Drug testing three dimensions
Ano:2022
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso restrito
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho
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author Caballero, David
author2 Kundu, B.
Abreu, Catarina M.
Amorim, S.
Fernandes, D. C.
Pires, R. A.
Oliveira, Joaquim M.
Correlo, V. M.
Reis, R. L.
Kundu, Subhas C
author2_role author
author
author
author
author
author
author
author
author
author_facet Caballero, David
Kundu, B.
Abreu, Catarina M.
Amorim, S.
Fernandes, D. C.
Pires, R. A.
Oliveira, Joaquim M.
Correlo, V. M.
Reis, R. L.
Kundu, Subhas C
author_role author
contributor_name_str_mv Universidade do Minho
country_str PT
creators_json_txt [{\"Person.name\":\"Caballero, David\"},{\"Person.name\":\"Kundu, B.\"},{\"Person.name\":\"Abreu, Catarina M.\"},{\"Person.name\":\"Amorim, S.\"},{\"Person.name\":\"Fernandes, D. C.\"},{\"Person.name\":\"Pires, R. A.\"},{\"Person.name\":\"Oliveira, Joaquim M.\"},{\"Person.name\":\"Correlo, V. M.\"},{\"Person.name\":\"Reis, R. L.\"},{\"Person.name\":\"Kundu, Subhas C\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Universidade do Minho
datacite.creators.creator.creatorName.fl_str_mv Caballero, David
Kundu, B.
Abreu, Catarina M.
Amorim, S.
Fernandes, D. C.
Pires, R. A.
Oliveira, Joaquim M.
Correlo, V. M.
Reis, R. L.
Kundu, Subhas C
datacite.date.Accepted.fl_str_mv 2022-03-01T00:00:00Z
datacite.date.embargoed.fl_str_mv 10000-01-01T00:00:00Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_16ec
datacite.subjects.subject.fl_str_mv 3D in vitro models
Cancer
drug screening
Drug testing
three dimensions
datacite.titles.title.fl_str_mv Forecast cancer: the importance of biomimetic 3D in vitro models in cancer drug testing/discovery and therapy
dc.contributor.none.fl_str_mv Universidade do Minho
dc.creator.none.fl_str_mv Caballero, David
Kundu, B.
Abreu, Catarina M.
Amorim, S.
Fernandes, D. C.
Pires, R. A.
Oliveira, Joaquim M.
Correlo, V. M.
Reis, R. L.
Kundu, Subhas C
dc.date.Accepted.fl_str_mv 2022-03-01T00:00:00Z
dc.date.embargoed.fl_str_mv 10000-01-01T00:00:00Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv https://hdl.handle.net/1822/76682
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv Springer
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.subject.none.fl_str_mv 3D in vitro models
Cancer
drug screening
Drug testing
three dimensions
dc.title.fl_str_mv Forecast cancer: the importance of biomimetic 3D in vitro models in cancer drug testing/discovery and therapy
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description Nowadays, the pharmaceutical industry faces a significant challenge related to the high attrition rates of drugs, which is particularly astonishing in oncology [1]. Indeed, several studies have reported that about 95% of anti-cancer drugs tested in phase I fail to reach the market. The reasons for this decline may be very complex, but most likely, they are related to how potential drug candidates are tested, which is far from being optimal. Typically, drugs are evaluated using cancer cells seeded on conventionalâ flatâ tissue culture surfaces (e.g., Petri dishes, flasks, or multi-well plates), which cannot recapitulate the three-dimensional (3D) architecture, rich cellular content, and complex interactions of the native tumor microenvironment [2]. Under these oversimplistic conditions, cancer cells display extreme phenotypes and aberrant gene expression, producing a non-physiological response when exposed to drugs. Additionally, these conventional approaches cannot recapitulate many of the dynamic events occurring in the human body that are crucial in cancer dissemination, particularly fluid flow or gradient formation [3, 4]. Similarly, animal models (e.g., mice, pigs, or primates), besides being ethically controversial and expensive, do not mimic human physiology, particularly the immune system, which is critical during cancer progression and therapy response. Even though â humanizedâ mice have been reported to address this limitation, they are not available for the majority of research labs and are incompatible with high-throughput production, an imperative drug discovery/screening feature.
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id rum_b0f4d9cb03e2d30c4e79b7258a6dce48
identifier.url.fl_str_mv https://hdl.handle.net/1822/76682
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institution Universidade do Minho
instname_str Universidade do Minho
language eng
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oai_identifier_str oai:repositorium.uminho.pt:1822/76682
organization_str_mv urn:organizationAcronym:repositorium
person_str_mv Caballero, David
Kundu, B.
Abreu, Catarina M.
Amorim, S.
Fernandes, D. C.
Pires, R. A.
Oliveira, Joaquim M.
Correlo, V. M.
Reis, R. L.
Kundu, Subhas C
publishDate 2022
publisher.none.fl_str_mv Springer
reponame_str RepositóriUM - Universidade do Minho
repository_id_str urn:repositoryAcronym:rum
service_str_mv urn:repositoryAcronym:rum
spelling engSpringerporNowadays, the pharmaceutical industry faces a significant challenge related to the high attrition rates of drugs, which is particularly astonishing in oncology [1]. Indeed, several studies have reported that about 95% of anti-cancer drugs tested in phase I fail to reach the market. The reasons for this decline may be very complex, but most likely, they are related to how potential drug candidates are tested, which is far from being optimal. Typically, drugs are evaluated using cancer cells seeded on conventionalâ flatâ tissue culture surfaces (e.g., Petri dishes, flasks, or multi-well plates), which cannot recapitulate the three-dimensional (3D) architecture, rich cellular content, and complex interactions of the native tumor microenvironment [2]. Under these oversimplistic conditions, cancer cells display extreme phenotypes and aberrant gene expression, producing a non-physiological response when exposed to drugs. Additionally, these conventional approaches cannot recapitulate many of the dynamic events occurring in the human body that are crucial in cancer dissemination, particularly fluid flow or gradient formation [3, 4]. Similarly, animal models (e.g., mice, pigs, or primates), besides being ethically controversial and expensive, do not mimic human physiology, particularly the immune system, which is critical during cancer progression and therapy response. Even though â humanizedâ mice have been reported to address this limitation, they are not available for the majority of research labs and are incompatible with high-throughput production, an imperative drug discovery/screening feature.application/pdfporForecast cancer: the importance of biomimetic 3D in vitro models in cancer drug testing/discovery and therapyCaballero, DavidKundu, B.Abreu, Catarina M.Amorim, S.Fernandes, D. C.Pires, R. A.Oliveira, Joaquim M.Correlo, V. M.Reis, R. L.Kundu, Subhas CHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf2731-3441DOIIsPartOf10.1007/s44164-022-00014-z2022-032022-032022-03-29T08:02:36Z2022-03-01T00:00:00Z10000-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/76682http://purl.org/coar/access_right/c_16ecrestricted access3D in vitro modelsCancerdrug screeningDrug testingthree dimensions934935 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_f1cfapplication/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/b7515d06-0a44-4823-a729-e698446235a7/download
spellingShingle Forecast cancer: the importance of biomimetic 3D in vitro models in cancer drug testing/discovery and therapy
Caballero, David
3D in vitro models
Cancer
drug screening
Drug testing
three dimensions
status SINGLETON
subject.fl_str_mv 3D in vitro models
Cancer
drug screening
Drug testing
three dimensions
title Forecast cancer: the importance of biomimetic 3D in vitro models in cancer drug testing/discovery and therapy
title_full Forecast cancer: the importance of biomimetic 3D in vitro models in cancer drug testing/discovery and therapy
title_fullStr Forecast cancer: the importance of biomimetic 3D in vitro models in cancer drug testing/discovery and therapy
title_full_unstemmed Forecast cancer: the importance of biomimetic 3D in vitro models in cancer drug testing/discovery and therapy
title_short Forecast cancer: the importance of biomimetic 3D in vitro models in cancer drug testing/discovery and therapy
title_sort Forecast cancer: the importance of biomimetic 3D in vitro models in cancer drug testing/discovery and therapy
topic 3D in vitro models
Cancer
drug screening
Drug testing
three dimensions
topic_facet 3D in vitro models
Cancer
drug screening
Drug testing
three dimensions
url https://hdl.handle.net/1822/76682
visible 1