Publicação
Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development
| Resumo: | Specific interactions between fibroblast growth factors (Fgf1-22) and their tyrosine kinase receptors (FgfR1-4) activate different signalling pathways that are responsible for the biological processes in which Fgf signalling is implicated during embryonic development. In the chick, several Fgf ligands (Fgf2, 4, 8, 9, 10, 12, 13 and 18) and the four FgfRs (FgfR 1, 2, 3 and 4) have been reported to be expressed in the developing limb. The precise spatial and temporal expression of these transcripts is important to guide the limb bud to develop into a wing/leg. In this paper, we present a detailed and systematic analysis of the expression patterns of FgfR1, 2, 3 and 4 throughout chick wing development, by in situ hybridisation on whole mounts and sections. Moreover, we characterize for the first time the different isoforms of FGFR1-3 by analysing their differential expression in limb ectoderm and mesodermal tissues, using RT-PCR and in situ hybridisation on sections. Finally, isoform-specific sequences for FgfR1IIIb, FgfR1IIIc, FgfR3IIIb and FgfR3IIIc were determined and deposited in GenBank with the following accession numbers: GU053725, GU065444, GU053726, GU065445, respectively. |
|---|---|
| Autores principais: | Sheeba, Caroline J. |
| Outros Autores: | Andrade, Raquel P.; Duprez, Delphine; Palmeirim, Isabel |
| Assunto: | Animals Chick embryo Ectoderm Fibroblast growth factors Gene expression Gene expression profiling In situ hybridization Limb buds Mesoderm Molecular sequence data Receptors, Fibroblast growth factor Reverse transcriptase polymerase chain reaction Signal Transduction Wings, Animal Gene expression regulation, Developmental Fgf FgfRs limb chick |
| Ano: | 2010 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866270603578703872 |
|---|---|
| author | Sheeba, Caroline J. |
| author2 | Andrade, Raquel P. Duprez, Delphine Palmeirim, Isabel |
| author2_role | author author author |
| author_facet | Sheeba, Caroline J. Andrade, Raquel P. Duprez, Delphine Palmeirim, Isabel |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Sheeba, Caroline J.\"},{\"Person.name\":\"Andrade, Raquel P.\"},{\"Person.name\":\"Duprez, Delphine\"},{\"Person.name\":\"Palmeirim, Isabel\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Sheeba, Caroline J. Andrade, Raquel P. Duprez, Delphine Palmeirim, Isabel |
| datacite.date.Accepted.fl_str_mv | 2010-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2020-10-19T08:26:32Z |
| datacite.date.embargoed.fl_str_mv | 2020-10-19T08:26:32Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Animals Chick embryo Ectoderm Fibroblast growth factors Gene expression Gene expression profiling In situ hybridization Limb buds Mesoderm Molecular sequence data Receptors, Fibroblast growth factor Reverse transcriptase polymerase chain reaction Signal Transduction Wings, Animal Gene expression regulation, Developmental Fgf FgfRs limb chick |
| datacite.titles.title.fl_str_mv | Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Sheeba, Caroline J. Andrade, Raquel P. Duprez, Delphine Palmeirim, Isabel |
| dc.date.Accepted.fl_str_mv | 2010-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2020-10-19T08:26:32Z |
| dc.date.embargoed.fl_str_mv | 2020-10-19T08:26:32Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/67556 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | University of the Basque Country (UPV/EHU) |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Animals Chick embryo Ectoderm Fibroblast growth factors Gene expression Gene expression profiling In situ hybridization Limb buds Mesoderm Molecular sequence data Receptors, Fibroblast growth factor Reverse transcriptase polymerase chain reaction Signal Transduction Wings, Animal Gene expression regulation, Developmental Fgf FgfRs limb chick |
| dc.title.fl_str_mv | Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Specific interactions between fibroblast growth factors (Fgf1-22) and their tyrosine kinase receptors (FgfR1-4) activate different signalling pathways that are responsible for the biological processes in which Fgf signalling is implicated during embryonic development. In the chick, several Fgf ligands (Fgf2, 4, 8, 9, 10, 12, 13 and 18) and the four FgfRs (FgfR 1, 2, 3 and 4) have been reported to be expressed in the developing limb. The precise spatial and temporal expression of these transcripts is important to guide the limb bud to develop into a wing/leg. In this paper, we present a detailed and systematic analysis of the expression patterns of FgfR1, 2, 3 and 4 throughout chick wing development, by in situ hybridisation on whole mounts and sections. Moreover, we characterize for the first time the different isoforms of FGFR1-3 by analysing their differential expression in limb ectoderm and mesodermal tissues, using RT-PCR and in situ hybridisation on sections. Finally, isoform-specific sequences for FgfR1IIIb, FgfR1IIIc, FgfR3IIIb and FgfR3IIIc were determined and deposited in GenBank with the following accession numbers: GU053725, GU065444, GU053726, GU065445, respectively. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/f729833c-b198-4379-8a7e-891057e90115/download |
| id | rum_b5dfc5696bd3baa39ff9b6ec0d2ac0d6 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/67556 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/67556 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Sheeba, Caroline J. Andrade, Raquel P. Duprez, Delphine Palmeirim, Isabel |
| publishDate | 2010 |
| publisher.none.fl_str_mv | University of the Basque Country (UPV/EHU) |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engUniversity of the Basque Country (UPV/EHU)porSpecific interactions between fibroblast growth factors (Fgf1-22) and their tyrosine kinase receptors (FgfR1-4) activate different signalling pathways that are responsible for the biological processes in which Fgf signalling is implicated during embryonic development. In the chick, several Fgf ligands (Fgf2, 4, 8, 9, 10, 12, 13 and 18) and the four FgfRs (FgfR 1, 2, 3 and 4) have been reported to be expressed in the developing limb. The precise spatial and temporal expression of these transcripts is important to guide the limb bud to develop into a wing/leg. In this paper, we present a detailed and systematic analysis of the expression patterns of FgfR1, 2, 3 and 4 throughout chick wing development, by in situ hybridisation on whole mounts and sections. Moreover, we characterize for the first time the different isoforms of FGFR1-3 by analysing their differential expression in limb ectoderm and mesodermal tissues, using RT-PCR and in situ hybridisation on sections. Finally, isoform-specific sequences for FgfR1IIIb, FgfR1IIIc, FgfR3IIIb and FgfR3IIIc were determined and deposited in GenBank with the following accession numbers: GU053725, GU065444, GU053726, GU065445, respectively.application/pdfporComprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb developmentSheeba, Caroline J.Andrade, Raquel P.Duprez, DelphinePalmeirim, IsabelHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf0214-6282DOIIsPartOf10.1387/ijdb.092887cs2020-10-19T08:26:32Z20102010-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/67556http://purl.org/coar/access_right/c_abf2open accessAnimalsChick embryoEctodermFibroblast growth factorsGene expressionGene expression profilingIn situ hybridizationLimb budsMesodermMolecular sequence dataReceptors, Fibroblast growth factorReverse transcriptase polymerase chain reactionSignal TransductionWings, AnimalGene expression regulation, DevelopmentalFgfFgfRslimbchick5028129 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/f729833c-b198-4379-8a7e-891057e90115/download |
| spellingShingle | Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development Sheeba, Caroline J. Animals Chick embryo Ectoderm Fibroblast growth factors Gene expression Gene expression profiling In situ hybridization Limb buds Mesoderm Molecular sequence data Receptors, Fibroblast growth factor Reverse transcriptase polymerase chain reaction Signal Transduction Wings, Animal Gene expression regulation, Developmental Fgf FgfRs limb chick |
| status | SINGLETON |
| subject.fl_str_mv | Animals Chick embryo Ectoderm Fibroblast growth factors Gene expression Gene expression profiling In situ hybridization Limb buds Mesoderm Molecular sequence data Receptors, Fibroblast growth factor Reverse transcriptase polymerase chain reaction Signal Transduction Wings, Animal Gene expression regulation, Developmental Fgf FgfRs limb chick |
| title | Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development |
| title_full | Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development |
| title_fullStr | Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development |
| title_full_unstemmed | Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development |
| title_short | Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development |
| title_sort | Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development |
| topic | Animals Chick embryo Ectoderm Fibroblast growth factors Gene expression Gene expression profiling In situ hybridization Limb buds Mesoderm Molecular sequence data Receptors, Fibroblast growth factor Reverse transcriptase polymerase chain reaction Signal Transduction Wings, Animal Gene expression regulation, Developmental Fgf FgfRs limb chick |
| topic_facet | Animals Chick embryo Ectoderm Fibroblast growth factors Gene expression Gene expression profiling In situ hybridization Limb buds Mesoderm Molecular sequence data Receptors, Fibroblast growth factor Reverse transcriptase polymerase chain reaction Signal Transduction Wings, Animal Gene expression regulation, Developmental Fgf FgfRs limb chick |
| url | https://hdl.handle.net/1822/67556 |
| visible | 1 |