Publicação

A case control study on the diagnostic potential of multiantigen serological tests for tuberculosis

Detalhes bibliográficos
Resumo:	Background: Eradicating tuberculosis (TB) in European countries with high incidence, such as Portugal, rely to a great extent on the ability to perform early accurate diagnosis. For the first time, the diagnostic value of two serologic tests, TB STAT-PAK II and MAPIA, was evaluated for rapid tuberculosis detection in a Portuguese cohort. Materials and Methods: A multicentre case-control study was performed on individuals who had been followed up for TB. Serum was collected from a total of 157 patients divided in: 1) control group with 32 healthy individuals 2) control group with 30 patients with pulmonary diseases other than TB; 3) patients with active TB on anti-TB medication for less than 2 weeks (43 patients), for 1 to 2 months (32 patients) or for more than 6 months (28 patients); 4) 25 patients with active TB co-infected with human immunodeficiency virus (HIV); 5) 29 recently exposed individuals with probable latent TB infection. Sera were tested for the presence of specific mycobacteria antibodies using the TB STAT-PAK II and MAPIA tests and compared with sputum smear microscopy information. Findings: Both of the serological methods showed high specificity. However the two methods had sub-optimal sensitivity, especially in HIV-co-infected patients. Combination of the antibody assays with sputum smear microscopy increased the overall sensitivity of rapid diagnosis. Conclusions: The use of point-of-care serodiagnostic tests may improve early detection of active tuberculosis and contribute for better control programs.
Autores principais:	Duarte, Raquel
Outros Autores:	Afonso, Andrea; Ferreira, Carina; Roque, Susana; Vasconcelos, Olga; Castro, Rui Sarmento; Tavares, Margarida; Caldas, Cátia; Greenwald, Rena; Lyashchenko, Konstantin; Neves, Margarida Correia; Nóbrega, Cláudia
Assunto:	Tuberculosis Diagnosis Serological tests
Ano:	2012
País:	Portugal
Tipo de documento:	artigo
Tipo de acesso:	acesso restrito
Instituição associada:	Universidade do Minho
Idioma:	inglês
Origem:	RepositóriUM - Universidade do Minho

Descrição
Resumo:	Background: Eradicating tuberculosis (TB) in European countries with high incidence, such as Portugal, rely to a great extent on the ability to perform early accurate diagnosis. For the first time, the diagnostic value of two serologic tests, TB STAT-PAK II and MAPIA, was evaluated for rapid tuberculosis detection in a Portuguese cohort. Materials and Methods: A multicentre case-control study was performed on individuals who had been followed up for TB. Serum was collected from a total of 157 patients divided in: 1) control group with 32 healthy individuals 2) control group with 30 patients with pulmonary diseases other than TB; 3) patients with active TB on anti-TB medication for less than 2 weeks (43 patients), for 1 to 2 months (32 patients) or for more than 6 months (28 patients); 4) 25 patients with active TB co-infected with human immunodeficiency virus (HIV); 5) 29 recently exposed individuals with probable latent TB infection. Sera were tested for the presence of specific mycobacteria antibodies using the TB STAT-PAK II and MAPIA tests and compared with sputum smear microscopy information. Findings: Both of the serological methods showed high specificity. However the two methods had sub-optimal sensitivity, especially in HIV-co-infected patients. Combination of the antibody assays with sputum smear microscopy increased the overall sensitivity of rapid diagnosis. Conclusions: The use of point-of-care serodiagnostic tests may improve early detection of active tuberculosis and contribute for better control programs.