Publication
Mutagenesis and transformation of Aspergillus terreus based on the nitrate reductase pathway
| Summary: | Aspergillus terreus is an efficient producer of different extracellular enzymes which are used in food industries. Thus, we are interested in developing a genetic transformation system based on the nitrate structural gene niaD. In order to develop this system the nitrate assimilation pathway in A. terreus was studied and spontaneous mutants defective in the genes required for nitrate assimilation were obtained on the basis of chlorate resistance. Of particular interest were niaD mutants which failed to grow on nitrate but grew as wild type on other sole nitrogen sources. Afterwards the transformation system was developed for mutants defective in nitrate reductase of A. terreus using pSTA10 vector containing niaD gene from A. niger. The transformation frequency obtained was c.a. 0.5 per ug DNA. The vector appeared to be mitotically stable and Southern hybridization analysis of transformants showed that transformation events occurred by integration into to the recipient genome. To assess the amount of product made by the transformants, nitrate reductase assays were carried out under inducing conditions. Experiments to improved transformation frequency and to characterize the integration are now in progress. |
|---|---|
| Main Authors: | Ferraz, Elza |
| Other Authors: | Lima, Nelson; Mota, M. |
| Year: | 1996 |
| Country: | Portugal |
| Document type: | other |
| Access type: | open access |
| Associated institution: | Universidade do Minho |
| Language: | English |
| Origin: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439055047753728 |
|---|---|
| author | Ferraz, Elza |
| author2 | Lima, Nelson Mota, M. |
| author2_role | author author |
| author_facet | Ferraz, Elza Lima, Nelson Mota, M. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Ferraz, Elza\"},{\"Person.name\":\"Lima, Nelson\"},{\"Person.name\":\"Mota, M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Ferraz, Elza Lima, Nelson Mota, M. |
| datacite.date.Accepted.fl_str_mv | 1996-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2006-01-06T15:41:17Z |
| datacite.date.embargoed.fl_str_mv | 2006-01-06T15:41:17Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.titles.title.fl_str_mv | Mutagenesis and transformation of Aspergillus terreus based on the nitrate reductase pathway |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Ferraz, Elza Lima, Nelson Mota, M. |
| dc.date.Accepted.fl_str_mv | 1996-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2006-01-06T15:41:17Z |
| dc.date.embargoed.fl_str_mv | 2006-01-06T15:41:17Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/3839 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | University of Missouri. Fungal Genetics Stock Center |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.title.fl_str_mv | Mutagenesis and transformation of Aspergillus terreus based on the nitrate reductase pathway |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_1843 |
| description | Aspergillus terreus is an efficient producer of different extracellular enzymes which are used in food industries. Thus, we are interested in developing a genetic transformation system based on the nitrate structural gene niaD. In order to develop this system the nitrate assimilation pathway in A. terreus was studied and spontaneous mutants defective in the genes required for nitrate assimilation were obtained on the basis of chlorate resistance. Of particular interest were niaD mutants which failed to grow on nitrate but grew as wild type on other sole nitrogen sources. Afterwards the transformation system was developed for mutants defective in nitrate reductase of A. terreus using pSTA10 vector containing niaD gene from A. niger. The transformation frequency obtained was c.a. 0.5 per ug DNA. The vector appeared to be mitotically stable and Southern hybridization analysis of transformants showed that transformation events occurred by integration into to the recipient genome. To assess the amount of product made by the transformants, nitrate reductase assays were carried out under inducing conditions. Experiments to improved transformation frequency and to characterize the integration are now in progress. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | other |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/1fcf73f7-6ff6-4448-a3ea-d81292e8b777/download |
| id | rum_b862048a2a51dfd06e6a17b2a5d222ca |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/3839 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/3839 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Ferraz, Elza Lima, Nelson Mota, M. |
| publishDate | 1996 |
| publisher.none.fl_str_mv | University of Missouri. Fungal Genetics Stock Center |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engUniversity of Missouri. Fungal Genetics Stock CenterengAspergillus terreus is an efficient producer of different extracellular enzymes which are used in food industries. Thus, we are interested in developing a genetic transformation system based on the nitrate structural gene niaD. In order to develop this system the nitrate assimilation pathway in A. terreus was studied and spontaneous mutants defective in the genes required for nitrate assimilation were obtained on the basis of chlorate resistance. Of particular interest were niaD mutants which failed to grow on nitrate but grew as wild type on other sole nitrogen sources. Afterwards the transformation system was developed for mutants defective in nitrate reductase of A. terreus using pSTA10 vector containing niaD gene from A. niger. The transformation frequency obtained was c.a. 0.5 per ug DNA. The vector appeared to be mitotically stable and Southern hybridization analysis of transformants showed that transformation events occurred by integration into to the recipient genome. To assess the amount of product made by the transformants, nitrate reductase assays were carried out under inducing conditions. Experiments to improved transformation frequency and to characterize the integration are now in progress.application/pdfengMutagenesis and transformation of Aspergillus terreus based on the nitrate reductase pathwayFerraz, ElzaLima, NelsonMota, M.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONFERRAZ, Elza; LIMA, Nelson; MOTA, Manuel - Mutagenesis and transformation of Aspergillus terreus based on the nitrate reductase pathway. In European Congress on Fungal Genetics, 3, Munster, 1996 – “On-line abstracts from the European Congress on Fungal Genetics 3, held March 27-30, 1996, Munster, Germany” [Em linha]. Kansas City : University of Missouri. Fungal Genetics Stock Center, [c. 1996]. [Consult. 6 Jan. 2006]. Disponível em: http://www.fgsc.net/ecfg/ecfg3.html2006-01-06T15:41:17Z19961996-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/3839http://purl.org/coar/access_right/c_abf2open access12650 bytesother research producthttp://purl.org/coar/resource_type/c_1843otherhttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/1fcf73f7-6ff6-4448-a3ea-d81292e8b777/download |
| spellingShingle | Mutagenesis and transformation of Aspergillus terreus based on the nitrate reductase pathway Ferraz, Elza |
| status | SINGLETON |
| title | Mutagenesis and transformation of Aspergillus terreus based on the nitrate reductase pathway |
| title_full | Mutagenesis and transformation of Aspergillus terreus based on the nitrate reductase pathway |
| title_fullStr | Mutagenesis and transformation of Aspergillus terreus based on the nitrate reductase pathway |
| title_full_unstemmed | Mutagenesis and transformation of Aspergillus terreus based on the nitrate reductase pathway |
| title_short | Mutagenesis and transformation of Aspergillus terreus based on the nitrate reductase pathway |
| title_sort | Mutagenesis and transformation of Aspergillus terreus based on the nitrate reductase pathway |
| url | https://hdl.handle.net/1822/3839 |
| visible | 1 |