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Hypericum perforatum and Its potential antiplatelet effect

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Resumo:Background: <i>Hypericum perforatum</i> (HP) is currently one of the most consumed medicinal plants in the world. In traditional Chinese medicine, the herb hypericum (<i>Guan Ye Lian Qiao</i>) belongs to the group of plants that clarify heat. It is also used to treat various types of infection and inflammation. In contrast to the extensive literature on the antidepressant effects of HP, little is known about its action on platelets. The main objective of this work was to investigate the possible relevance of HP to platelet function. Methods: We characterized the profile of platelet activation in the presence of HP extracts through an evaluation of molecular markers by flow cytometry: mobilization of intracellular Ca++ and expression of platelet receptors such as activated GPIIbIIIa and P-selectin (CD62). Results: The results indicated a possible inhibitory effect of HP on the platelet activation response, which could be explained by the effect on intracellular calcium mobilization and the expression of activated GPIIbIIIa receptors. Despite of the limitations of an in vitro study, our results provide evidence of the possible mechanisms of action of HP. Conclusions: Further studies are needed to elucidate the effect of HP on hemostasis, but it may be recognized as a substance with antiplatelet properties.
Autores principais:Monteiro, Maria-do-Céu
Outros Autores:Dias, Alberto Carlos Pires; Costa, Daniela; Almeida-Dias, António; Criado, Maria Begoña
Assunto:Hypericum perforatum platelet activation flow cytometry traditional Chinese medicine traditional Chinese medicine
Ano:2022
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho
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author Monteiro, Maria-do-Céu
author2 Dias, Alberto Carlos Pires
Costa, Daniela
Almeida-Dias, António
Criado, Maria Begoña
author2_role author
author
author
author
author_facet Monteiro, Maria-do-Céu
Dias, Alberto Carlos Pires
Costa, Daniela
Almeida-Dias, António
Criado, Maria Begoña
author_role author
contributor_name_str_mv Universidade do Minho
country_str PT
creators_json_txt [{\"Person.name\":\"Monteiro, Maria-do-Céu\"},{\"Person.name\":\"Dias, Alberto Carlos Pires\"},{\"Person.name\":\"Costa, Daniela\"},{\"Person.name\":\"Almeida-Dias, António\"},{\"Person.name\":\"Criado, Maria Begoña\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Universidade do Minho
datacite.creators.creator.creatorName.fl_str_mv Monteiro, Maria-do-Céu
Dias, Alberto Carlos Pires
Costa, Daniela
Almeida-Dias, António
Criado, Maria Begoña
datacite.date.Accepted.fl_str_mv 2022-09-15T00:00:00Z
datacite.date.available.fl_str_mv 2022-11-21T10:55:48Z
datacite.date.embargoed.fl_str_mv 2022-11-21T10:55:48Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv Hypericum perforatum
platelet activation
flow cytometry
traditional Chinese
medicine
traditional Chinese medicine
datacite.titles.title.fl_str_mv Hypericum perforatum and Its potential antiplatelet effect
dc.contributor.none.fl_str_mv Universidade do Minho
dc.creator.none.fl_str_mv Monteiro, Maria-do-Céu
Dias, Alberto Carlos Pires
Costa, Daniela
Almeida-Dias, António
Criado, Maria Begoña
dc.date.Accepted.fl_str_mv 2022-09-15T00:00:00Z
dc.date.available.fl_str_mv 2022-11-21T10:55:48Z
dc.date.embargoed.fl_str_mv 2022-11-21T10:55:48Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv https://hdl.handle.net/1822/80744
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.rights.cclincense.fl_str_mv http://creativecommons.org/licenses/by/4.0/
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.rights.copyright.fl_str_mv openAccess
dc.subject.none.fl_str_mv Hypericum perforatum
platelet activation
flow cytometry
traditional Chinese
medicine
traditional Chinese medicine
dc.title.fl_str_mv Hypericum perforatum and Its potential antiplatelet effect
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description Background: <i>Hypericum perforatum</i> (HP) is currently one of the most consumed medicinal plants in the world. In traditional Chinese medicine, the herb hypericum (<i>Guan Ye Lian Qiao</i>) belongs to the group of plants that clarify heat. It is also used to treat various types of infection and inflammation. In contrast to the extensive literature on the antidepressant effects of HP, little is known about its action on platelets. The main objective of this work was to investigate the possible relevance of HP to platelet function. Methods: We characterized the profile of platelet activation in the presence of HP extracts through an evaluation of molecular markers by flow cytometry: mobilization of intracellular Ca++ and expression of platelet receptors such as activated GPIIbIIIa and P-selectin (CD62). Results: The results indicated a possible inhibitory effect of HP on the platelet activation response, which could be explained by the effect on intracellular calcium mobilization and the expression of activated GPIIbIIIa receptors. Despite of the limitations of an in vitro study, our results provide evidence of the possible mechanisms of action of HP. Conclusions: Further studies are needed to elucidate the effect of HP on hemostasis, but it may be recognized as a substance with antiplatelet properties.
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eu_rights_str_mv openAccess
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fulltext.url.fl_str_mv https://repositorium.uminho.pt/bitstreams/a945f472-b04f-4af2-9556-8037b192e1a8/download
id rum_b8c76ec39f623ca71bc3d2b41ba77bf3
identifier.url.fl_str_mv https://hdl.handle.net/1822/80744
instacron_str repositorium
institution Universidade do Minho
instname_str Universidade do Minho
language eng
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oai_identifier_str oai:repositorium.uminho.pt:1822/80744
organization_str_mv urn:organizationAcronym:repositorium
person_str_mv Monteiro, Maria-do-Céu
Dias, Alberto Carlos Pires
Costa, Daniela
Almeida-Dias, António
Criado, Maria Begoña
publishDate 2022
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
reponame_str RepositóriUM - Universidade do Minho
repository_id_str urn:repositoryAcronym:rum
service_str_mv urn:repositoryAcronym:rum
spelling engMultidisciplinary Digital Publishing InstituteporBackground: <i>Hypericum perforatum</i> (HP) is currently one of the most consumed medicinal plants in the world. In traditional Chinese medicine, the herb hypericum (<i>Guan Ye Lian Qiao</i>) belongs to the group of plants that clarify heat. It is also used to treat various types of infection and inflammation. In contrast to the extensive literature on the antidepressant effects of HP, little is known about its action on platelets. The main objective of this work was to investigate the possible relevance of HP to platelet function. Methods: We characterized the profile of platelet activation in the presence of HP extracts through an evaluation of molecular markers by flow cytometry: mobilization of intracellular Ca++ and expression of platelet receptors such as activated GPIIbIIIa and P-selectin (CD62). Results: The results indicated a possible inhibitory effect of HP on the platelet activation response, which could be explained by the effect on intracellular calcium mobilization and the expression of activated GPIIbIIIa receptors. Despite of the limitations of an in vitro study, our results provide evidence of the possible mechanisms of action of HP. Conclusions: Further studies are needed to elucidate the effect of HP on hemostasis, but it may be recognized as a substance with antiplatelet properties.application/pdfengHypericum perforatum and Its potential antiplatelet effectMonteiro, Maria-do-CéuDias, Alberto Carlos PiresCosta, DanielaAlmeida-Dias, AntónioCriado, Maria BegoñaHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptDOIIsPartOf10.3390/healthcare100917742022-11-21T10:55:48Z2022-09-152022-09-22T12:02:38Z2022-09-15T00:00:00ZHandlehttps://hdl.handle.net/1822/80744http://purl.org/coar/access_right/c_abf2open accessHypericum perforatumplatelet activationflow cytometrytraditional Chinesemedicinetraditional Chinese medicine1531829 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2022-09-15http://creativecommons.org/licenses/by/4.0/openAccesshttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/a945f472-b04f-4af2-9556-8037b192e1a8/download
spellingShingle Hypericum perforatum and Its potential antiplatelet effect
Monteiro, Maria-do-Céu
Hypericum perforatum
platelet activation
flow cytometry
traditional Chinese
medicine
traditional Chinese medicine
status SINGLETON
subject.fl_str_mv Hypericum perforatum
platelet activation
flow cytometry
traditional Chinese
medicine
traditional Chinese medicine
title Hypericum perforatum and Its potential antiplatelet effect
title_full Hypericum perforatum and Its potential antiplatelet effect
title_fullStr Hypericum perforatum and Its potential antiplatelet effect
title_full_unstemmed Hypericum perforatum and Its potential antiplatelet effect
title_short Hypericum perforatum and Its potential antiplatelet effect
title_sort Hypericum perforatum and Its potential antiplatelet effect
topic Hypericum perforatum
platelet activation
flow cytometry
traditional Chinese
medicine
traditional Chinese medicine
topic_facet Hypericum perforatum
platelet activation
flow cytometry
traditional Chinese
medicine
traditional Chinese medicine
url https://hdl.handle.net/1822/80744
visible 1