Publicação
Biocompatibility of poly(lactic acid) with incorporated graphene-based materials
| Resumo: | The incorporation of graphene-based materials has been shown to improve mechanical properties of poly(lactic acid) (PLA). In this work, PLA films and composite PLA films incorporating two graphene-based materials – graphene oxide (GO) and graphene nanoplatelets (GNP) – were prepared and characterized regarding not only biocompatibility, but also surface topography, chemistry and wettability. The presence of both fillers changed the films surface topography, increasing the roughness, and modified the wettability – the polar component of surface free energy increased 59% with GO and decreased 56% with GNP. Mouse embryo fibroblasts incubated with both fillers exceeded the IC50 in both cases with a concentration of 10 μg mL−1. No variations in cell proliferation at the surface of the composite films were observed, except for those containing GO after 24 h incubation, which presented higher cell proliferation than pristine PLA films. Platelet adhesion to PLA and PLA/GNP films was lower in the presence of plasma proteins than when no proteins were present. Furthermore, incorporation of GNP into PLA reduced platelet activation in the presence of plasma proteins. The results indicated that low concentrations of GO and GNP may be incorporated safely in PLA to improve aspects relevant for biomedical applications, such as mechanical properties. |
|---|---|
| Autores principais: | Pinto, Artur Moreira |
| Outros Autores: | Moreira, Susana Margarida Gomes; Gonçalves, Inês; Gama, F. M.; Mendes, Adélio; Magalhães, Fernão D. |
| Assunto: | Topography Wettability Cytotoxicity Platelets Hemocompatibility |
| Ano: | 2013 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867438927537766400 |
|---|---|
| author | Pinto, Artur Moreira |
| author2 | Moreira, Susana Margarida Gomes Gonçalves, Inês Gama, F. M. Mendes, Adélio Magalhães, Fernão D. |
| author2_role | author author author author author |
| author_facet | Pinto, Artur Moreira Moreira, Susana Margarida Gomes Gonçalves, Inês Gama, F. M. Mendes, Adélio Magalhães, Fernão D. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Pinto, Artur Moreira\"},{\"Person.name\":\"Moreira, Susana Margarida Gomes\"},{\"Person.name\":\"Gonçalves, Inês\"},{\"Person.name\":\"Gama, F. M.\"},{\"Person.name\":\"Mendes, Adélio\"},{\"Person.name\":\"Magalhães, Fernão D.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Pinto, Artur Moreira Moreira, Susana Margarida Gomes Gonçalves, Inês Gama, F. M. Mendes, Adélio Magalhães, Fernão D. |
| datacite.date.Accepted.fl_str_mv | 2013-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2013-06-05T14:13:32Z |
| datacite.date.embargoed.fl_str_mv | 2013-06-05T14:13:32Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Topography Wettability Cytotoxicity Platelets Hemocompatibility |
| datacite.titles.title.fl_str_mv | Biocompatibility of poly(lactic acid) with incorporated graphene-based materials |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Pinto, Artur Moreira Moreira, Susana Margarida Gomes Gonçalves, Inês Gama, F. M. Mendes, Adélio Magalhães, Fernão D. |
| dc.date.Accepted.fl_str_mv | 2013-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2013-06-05T14:13:32Z |
| dc.date.embargoed.fl_str_mv | 2013-06-05T14:13:32Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/24335 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Topography Wettability Cytotoxicity Platelets Hemocompatibility |
| dc.title.fl_str_mv | Biocompatibility of poly(lactic acid) with incorporated graphene-based materials |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The incorporation of graphene-based materials has been shown to improve mechanical properties of poly(lactic acid) (PLA). In this work, PLA films and composite PLA films incorporating two graphene-based materials – graphene oxide (GO) and graphene nanoplatelets (GNP) – were prepared and characterized regarding not only biocompatibility, but also surface topography, chemistry and wettability. The presence of both fillers changed the films surface topography, increasing the roughness, and modified the wettability – the polar component of surface free energy increased 59% with GO and decreased 56% with GNP. Mouse embryo fibroblasts incubated with both fillers exceeded the IC50 in both cases with a concentration of 10 μg mL−1. No variations in cell proliferation at the surface of the composite films were observed, except for those containing GO after 24 h incubation, which presented higher cell proliferation than pristine PLA films. Platelet adhesion to PLA and PLA/GNP films was lower in the presence of plasma proteins than when no proteins were present. Furthermore, incorporation of GNP into PLA reduced platelet activation in the presence of plasma proteins. The results indicated that low concentrations of GO and GNP may be incorporated safely in PLA to improve aspects relevant for biomedical applications, such as mechanical properties. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/f2ab7d94-e072-4455-976c-ca739e077e3b/download |
| id | rum_bd1c8e2d2cc7abb151bc279c68c5fdb2 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/24335 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/24335 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Pinto, Artur Moreira Moreira, Susana Margarida Gomes Gonçalves, Inês Gama, F. M. Mendes, Adélio Magalhães, Fernão D. |
| publishDate | 2013 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engElsevierporThe incorporation of graphene-based materials has been shown to improve mechanical properties of poly(lactic acid) (PLA). In this work, PLA films and composite PLA films incorporating two graphene-based materials – graphene oxide (GO) and graphene nanoplatelets (GNP) – were prepared and characterized regarding not only biocompatibility, but also surface topography, chemistry and wettability. The presence of both fillers changed the films surface topography, increasing the roughness, and modified the wettability – the polar component of surface free energy increased 59% with GO and decreased 56% with GNP. Mouse embryo fibroblasts incubated with both fillers exceeded the IC50 in both cases with a concentration of 10 μg mL−1. No variations in cell proliferation at the surface of the composite films were observed, except for those containing GO after 24 h incubation, which presented higher cell proliferation than pristine PLA films. Platelet adhesion to PLA and PLA/GNP films was lower in the presence of plasma proteins than when no proteins were present. Furthermore, incorporation of GNP into PLA reduced platelet activation in the presence of plasma proteins. The results indicated that low concentrations of GO and GNP may be incorporated safely in PLA to improve aspects relevant for biomedical applications, such as mechanical properties.application/pdfporBiocompatibility of poly(lactic acid) with incorporated graphene-based materialsPinto, Artur MoreiraMoreira, Susana Margarida GomesGonçalves, InêsGama, F. M.Mendes, AdélioMagalhães, Fernão D.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptPMID23333912ISSNIsPartOf0927-7765DOIIsPartOf10.1016/j.colsurfb.2012.12.0062013-06-05T14:13:32Z20132013-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/24335http://purl.org/coar/access_right/c_abf2open accessTopographyWettabilityCytotoxicityPlateletsHemocompatibility1853463 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/f2ab7d94-e072-4455-976c-ca739e077e3b/download |
| spellingShingle | Biocompatibility of poly(lactic acid) with incorporated graphene-based materials Pinto, Artur Moreira Topography Wettability Cytotoxicity Platelets Hemocompatibility |
| status | SINGLETON |
| subject.fl_str_mv | Topography Wettability Cytotoxicity Platelets Hemocompatibility |
| title | Biocompatibility of poly(lactic acid) with incorporated graphene-based materials |
| title_full | Biocompatibility of poly(lactic acid) with incorporated graphene-based materials |
| title_fullStr | Biocompatibility of poly(lactic acid) with incorporated graphene-based materials |
| title_full_unstemmed | Biocompatibility of poly(lactic acid) with incorporated graphene-based materials |
| title_short | Biocompatibility of poly(lactic acid) with incorporated graphene-based materials |
| title_sort | Biocompatibility of poly(lactic acid) with incorporated graphene-based materials |
| topic | Topography Wettability Cytotoxicity Platelets Hemocompatibility |
| topic_facet | Topography Wettability Cytotoxicity Platelets Hemocompatibility |
| url | https://hdl.handle.net/1822/24335 |
| visible | 1 |