Publicação
Polyamide microparticles with immobilized enological pectinase as efficient biocatalysts for wine clarification: the role of the polymer support
| Resumo: | Free pectinase is commonly employed as a biocatalyst in wine clarification; however, its removal, recovery, and reuse are not feasible. To address these limitations, this study focuses on the immobilization of a commercial pectinolytic preparation (Pec) onto highly porous polymer microparticles (MPs). Seven microparticulate polyamide (PA) supports, namely PA4, PA6, PA12 (with and without magnetic properties), and the copolymeric PA612 MP, were synthesized through activated anionic ring-opening polymerization of various lactams. Pectinase was non-covalently immobilized on these supports by adsorption, forming Pec@PA conjugates. Comparative activity and kinetic studies revealed that the Pec@PA12 conjugate exhibited more than twice the catalytic efficiency of the free enzyme, followed by Pec@PA6-Fe and Pec@PA4-Fe. All Pec@PA complexes were tested in the clarification of industrial rosé must, demonstrating similar or better performance compared to the free enzyme. Some immobilized biocatalysts supported up to seven consecutive reuse cycles, maintaining up to 50% of their initial activity and achieving complete clarification within 3–30 h across three consecutive cycles of application. These findings highlight the potential for industrial applications of noncovalently immobilized pectinase on various polyamide microparticles, with possibilities for customization of the conjugates’ properties. |
|---|---|
| Autores principais: | Oliveira, Sandra Cristina Gomes |
| Outros Autores: | Araújo, Samuel M.; Dencheva, Nadya Vasileva; Denchev, Z. |
| Assunto: | Wine clarification Pectinase Enzyme immobilization Anionic polymerization of lactams Biocatalyst reusability PA4 PA6 PA12 |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1864252108165873664 |
|---|---|
| author | Oliveira, Sandra Cristina Gomes |
| author2 | Araújo, Samuel M. Dencheva, Nadya Vasileva Denchev, Z. |
| author2_role | author author author |
| author_facet | Oliveira, Sandra Cristina Gomes Araújo, Samuel M. Dencheva, Nadya Vasileva Denchev, Z. |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Oliveira, Sandra Cristina Gomes\"},{\"Person.name\":\"Araújo, Samuel M.\"},{\"Person.name\":\"Dencheva, Nadya Vasileva\"},{\"Person.name\":\"Denchev, Z.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Oliveira, Sandra Cristina Gomes Araújo, Samuel M. Dencheva, Nadya Vasileva Denchev, Z. |
| datacite.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2025-01-06T09:47:08Z |
| datacite.date.embargoed.fl_str_mv | 2025-01-06T09:47:08Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Wine clarification Pectinase Enzyme immobilization Anionic polymerization of lactams Biocatalyst reusability PA4 PA6 PA12 |
| datacite.titles.title.fl_str_mv | Polyamide microparticles with immobilized enological pectinase as efficient biocatalysts for wine clarification: the role of the polymer support |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Oliveira, Sandra Cristina Gomes Araújo, Samuel M. Dencheva, Nadya Vasileva Denchev, Z. |
| dc.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2025-01-06T09:47:08Z |
| dc.date.embargoed.fl_str_mv | 2025-01-06T09:47:08Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/94166 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Multidisciplinary Digital Publishing Institute (MDPI) |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.rights.rights.copyright.fl_str_mv | openAccess |
| dc.subject.none.fl_str_mv | Wine clarification Pectinase Enzyme immobilization Anionic polymerization of lactams Biocatalyst reusability PA4 PA6 PA12 |
| dc.title.fl_str_mv | Polyamide microparticles with immobilized enological pectinase as efficient biocatalysts for wine clarification: the role of the polymer support |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Free pectinase is commonly employed as a biocatalyst in wine clarification; however, its removal, recovery, and reuse are not feasible. To address these limitations, this study focuses on the immobilization of a commercial pectinolytic preparation (Pec) onto highly porous polymer microparticles (MPs). Seven microparticulate polyamide (PA) supports, namely PA4, PA6, PA12 (with and without magnetic properties), and the copolymeric PA612 MP, were synthesized through activated anionic ring-opening polymerization of various lactams. Pectinase was non-covalently immobilized on these supports by adsorption, forming Pec@PA conjugates. Comparative activity and kinetic studies revealed that the Pec@PA12 conjugate exhibited more than twice the catalytic efficiency of the free enzyme, followed by Pec@PA6-Fe and Pec@PA4-Fe. All Pec@PA complexes were tested in the clarification of industrial rosé must, demonstrating similar or better performance compared to the free enzyme. Some immobilized biocatalysts supported up to seven consecutive reuse cycles, maintaining up to 50% of their initial activity and achieving complete clarification within 3–30 h across three consecutive cycles of application. These findings highlight the potential for industrial applications of noncovalently immobilized pectinase on various polyamide microparticles, with possibilities for customization of the conjugates’ properties. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/fa58cad7-a9c8-498e-9150-f84b2829873a/download |
| id | rum_bdf475ea00a84ce1a2b3182e112b64e4 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/94166 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/94166 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Oliveira, Sandra Cristina Gomes Araújo, Samuel M. Dencheva, Nadya Vasileva Denchev, Z. |
| publishDate | 2025 |
| publisher.none.fl_str_mv | Multidisciplinary Digital Publishing Institute (MDPI) |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engMultidisciplinary Digital Publishing Institute (MDPI)porFree pectinase is commonly employed as a biocatalyst in wine clarification; however, its removal, recovery, and reuse are not feasible. To address these limitations, this study focuses on the immobilization of a commercial pectinolytic preparation (Pec) onto highly porous polymer microparticles (MPs). Seven microparticulate polyamide (PA) supports, namely PA4, PA6, PA12 (with and without magnetic properties), and the copolymeric PA612 MP, were synthesized through activated anionic ring-opening polymerization of various lactams. Pectinase was non-covalently immobilized on these supports by adsorption, forming Pec@PA conjugates. Comparative activity and kinetic studies revealed that the Pec@PA12 conjugate exhibited more than twice the catalytic efficiency of the free enzyme, followed by Pec@PA6-Fe and Pec@PA4-Fe. All Pec@PA complexes were tested in the clarification of industrial rosé must, demonstrating similar or better performance compared to the free enzyme. Some immobilized biocatalysts supported up to seven consecutive reuse cycles, maintaining up to 50% of their initial activity and achieving complete clarification within 3–30 h across three consecutive cycles of application. These findings highlight the potential for industrial applications of noncovalently immobilized pectinase on various polyamide microparticles, with possibilities for customization of the conjugates’ properties.application/pdfporPolyamide microparticles with immobilized enological pectinase as efficient biocatalysts for wine clarification: the role of the polymer supportOliveira, Sandra Cristina GomesAraújo, Samuel M.Dencheva, Nadya VasilevaDenchev, Z.HostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptDOIIsPartOf10.3390/molecules300101142025-01-06T09:47:08Z20252025-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/94166http://purl.org/coar/access_right/c_abf2open accessWine clarificationPectinaseEnzyme immobilizationAnionic polymerization of lactamsBiocatalyst reusabilityPA4PA6PA124183057 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2025http://creativecommons.org/licenses/by/4.0/openAccesshttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/fa58cad7-a9c8-498e-9150-f84b2829873a/download |
| spellingShingle | Polyamide microparticles with immobilized enological pectinase as efficient biocatalysts for wine clarification: the role of the polymer support Oliveira, Sandra Cristina Gomes Wine clarification Pectinase Enzyme immobilization Anionic polymerization of lactams Biocatalyst reusability PA4 PA6 PA12 |
| subject.fl_str_mv | Wine clarification Pectinase Enzyme immobilization Anionic polymerization of lactams Biocatalyst reusability PA4 PA6 PA12 |
| title | Polyamide microparticles with immobilized enological pectinase as efficient biocatalysts for wine clarification: the role of the polymer support |
| title_full | Polyamide microparticles with immobilized enological pectinase as efficient biocatalysts for wine clarification: the role of the polymer support |
| title_fullStr | Polyamide microparticles with immobilized enological pectinase as efficient biocatalysts for wine clarification: the role of the polymer support |
| title_full_unstemmed | Polyamide microparticles with immobilized enological pectinase as efficient biocatalysts for wine clarification: the role of the polymer support |
| title_short | Polyamide microparticles with immobilized enological pectinase as efficient biocatalysts for wine clarification: the role of the polymer support |
| title_sort | Polyamide microparticles with immobilized enological pectinase as efficient biocatalysts for wine clarification: the role of the polymer support |
| topic | Wine clarification Pectinase Enzyme immobilization Anionic polymerization of lactams Biocatalyst reusability PA4 PA6 PA12 |
| topic_facet | Wine clarification Pectinase Enzyme immobilization Anionic polymerization of lactams Biocatalyst reusability PA4 PA6 PA12 |
| url | https://hdl.handle.net/1822/94166 |
| visible | 1 |