Publicação
Suturable regenerated silk fibroin scaffold reinforced with 3D-printed polycaprolactone mesh: biomechanical performance and subcutaneous implantation
| Resumo: | The menisci have crucial roles in the knee, chondroprotection being the primary. Meniscus repair or substitution is favored in the clinical management of the meniscus lesions with given indications. The outstanding challenges with the meniscal scaffolds include the required biomechanical behavior and features. Suturability is one of the prerequisites for both implantation and implant survival. Therefore, we proposed herein a novel highly interconnected suturable porous scaffolds from regenerated silk fibroin that is reinforced with 3D-printed polycaprolactone (PCL) mesh in the middle, on the transverse plane to enhance the suture-holding capacity. Results showed that the reinforcement of the silk fibroin scaffolds with the PCL mesh increased the suture retention strength up to 400%, with a decrease in the mean porosity and an increase in crystallinity from 51.9 to 55.6%. The wet compression modulus values were significantly different for silk fibroin, and silk fibroin + PCL mesh by being 0.16 ± 0.02, and 0.40 ± 0.06 MPa, respectively. Both scaffolds had excellent interconnectivity (>99%), and a high water uptake feature (>500%). The tissueâ s infiltration and formation of new blood vessels were assessed by means of performing an in vivo subcutaneous implantation of the silk fibroin + PCL mesh scaffolds that were seeded with primary human meniscocytes or stem cells. Regarding suturability and in vivo biocompatibility, the findings of this study indicate that the silk fibroin + PCL mesh scaffolds are suitable for further studies to be carried out for meniscus tissue engineering applications such as the studies involving orthotopic meniscal models and fabrication of patient-specific implants. |
|---|---|
| Autores principais: | Cengiz, I. F. |
| Outros Autores: | Pereira, H.; Espregueira-Mendes, J.; Kwon, I. K.; Reis, R. L.; Oliveira, Joaquim M. |
| Assunto: | 3D-printed mesh meniscus Meniscus tissue engineering Silk Fibroin subcutaneous implantation Suturability Suture retention |
| Ano: | 2019 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866875486724947968 |
|---|---|
| author | Cengiz, I. F. |
| author2 | Pereira, H. Espregueira-Mendes, J. Kwon, I. K. Reis, R. L. Oliveira, Joaquim M. |
| author2_role | author author author author author |
| author_facet | Cengiz, I. F. Pereira, H. Espregueira-Mendes, J. Kwon, I. K. Reis, R. L. Oliveira, Joaquim M. |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Cengiz, I. F.\"},{\"Person.name\":\"Pereira, H.\"},{\"Person.name\":\"Espregueira-Mendes, J.\"},{\"Person.name\":\"Kwon, I. K.\"},{\"Person.name\":\"Reis, R. L.\"},{\"Person.name\":\"Oliveira, Joaquim M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Cengiz, I. F. Pereira, H. Espregueira-Mendes, J. Kwon, I. K. Reis, R. L. Oliveira, Joaquim M. |
| datacite.date.Accepted.fl_str_mv | 2019-05-01T00:00:00Z |
| datacite.date.embargoed.fl_str_mv | 10000-01-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | 3D-printed mesh meniscus Meniscus tissue engineering Silk Fibroin subcutaneous implantation Suturability Suture retention |
| datacite.titles.title.fl_str_mv | Suturable regenerated silk fibroin scaffold reinforced with 3D-printed polycaprolactone mesh: biomechanical performance and subcutaneous implantation |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Cengiz, I. F. Pereira, H. Espregueira-Mendes, J. Kwon, I. K. Reis, R. L. Oliveira, Joaquim M. |
| dc.date.Accepted.fl_str_mv | 2019-05-01T00:00:00Z |
| dc.date.embargoed.fl_str_mv | 10000-01-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/66387 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Springer |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | 3D-printed mesh meniscus Meniscus tissue engineering Silk Fibroin subcutaneous implantation Suturability Suture retention |
| dc.title.fl_str_mv | Suturable regenerated silk fibroin scaffold reinforced with 3D-printed polycaprolactone mesh: biomechanical performance and subcutaneous implantation |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The menisci have crucial roles in the knee, chondroprotection being the primary. Meniscus repair or substitution is favored in the clinical management of the meniscus lesions with given indications. The outstanding challenges with the meniscal scaffolds include the required biomechanical behavior and features. Suturability is one of the prerequisites for both implantation and implant survival. Therefore, we proposed herein a novel highly interconnected suturable porous scaffolds from regenerated silk fibroin that is reinforced with 3D-printed polycaprolactone (PCL) mesh in the middle, on the transverse plane to enhance the suture-holding capacity. Results showed that the reinforcement of the silk fibroin scaffolds with the PCL mesh increased the suture retention strength up to 400%, with a decrease in the mean porosity and an increase in crystallinity from 51.9 to 55.6%. The wet compression modulus values were significantly different for silk fibroin, and silk fibroin + PCL mesh by being 0.16 ± 0.02, and 0.40 ± 0.06 MPa, respectively. Both scaffolds had excellent interconnectivity (>99%), and a high water uptake feature (>500%). The tissueâ s infiltration and formation of new blood vessels were assessed by means of performing an in vivo subcutaneous implantation of the silk fibroin + PCL mesh scaffolds that were seeded with primary human meniscocytes or stem cells. Regarding suturability and in vivo biocompatibility, the findings of this study indicate that the silk fibroin + PCL mesh scaffolds are suitable for further studies to be carried out for meniscus tissue engineering applications such as the studies involving orthotopic meniscal models and fabrication of patient-specific implants. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/a5856f77-8278-4e1f-bf42-6c685abecd98/download |
| id | rum_bfb9bb40b231a43db210bc09fcc94f84 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/66387 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/66387 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Cengiz, I. F. Pereira, H. Espregueira-Mendes, J. Kwon, I. K. Reis, R. L. Oliveira, Joaquim M. |
| publishDate | 2019 |
| publisher.none.fl_str_mv | Springer |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engSpringerporThe menisci have crucial roles in the knee, chondroprotection being the primary. Meniscus repair or substitution is favored in the clinical management of the meniscus lesions with given indications. The outstanding challenges with the meniscal scaffolds include the required biomechanical behavior and features. Suturability is one of the prerequisites for both implantation and implant survival. Therefore, we proposed herein a novel highly interconnected suturable porous scaffolds from regenerated silk fibroin that is reinforced with 3D-printed polycaprolactone (PCL) mesh in the middle, on the transverse plane to enhance the suture-holding capacity. Results showed that the reinforcement of the silk fibroin scaffolds with the PCL mesh increased the suture retention strength up to 400%, with a decrease in the mean porosity and an increase in crystallinity from 51.9 to 55.6%. The wet compression modulus values were significantly different for silk fibroin, and silk fibroin + PCL mesh by being 0.16 ± 0.02, and 0.40 ± 0.06 MPa, respectively. Both scaffolds had excellent interconnectivity (>99%), and a high water uptake feature (>500%). The tissueâ s infiltration and formation of new blood vessels were assessed by means of performing an in vivo subcutaneous implantation of the silk fibroin + PCL mesh scaffolds that were seeded with primary human meniscocytes or stem cells. Regarding suturability and in vivo biocompatibility, the findings of this study indicate that the silk fibroin + PCL mesh scaffolds are suitable for further studies to be carried out for meniscus tissue engineering applications such as the studies involving orthotopic meniscal models and fabrication of patient-specific implants.application/pdfporSuturable regenerated silk fibroin scaffold reinforced with 3D-printed polycaprolactone mesh: biomechanical performance and subcutaneous implantationCengiz, I. F.Pereira, H.Espregueira-Mendes, J.Kwon, I. K.Reis, R. L.Oliveira, Joaquim M.HostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf1573-4838DOIIsPartOf10.1007/s10856-019-6265-32019-052019-062020-08-07T10:18:28Z2019-05-01T00:00:00Z10000-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/66387http://purl.org/coar/access_right/c_16ecrestricted access3D-printed meshmeniscusMeniscus tissue engineeringSilk Fibroinsubcutaneous implantationSuturabilitySuture retention5689129 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_f1cfapplication/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/a5856f77-8278-4e1f-bf42-6c685abecd98/download |
| spellingShingle | Suturable regenerated silk fibroin scaffold reinforced with 3D-printed polycaprolactone mesh: biomechanical performance and subcutaneous implantation Cengiz, I. F. 3D-printed mesh meniscus Meniscus tissue engineering Silk Fibroin subcutaneous implantation Suturability Suture retention |
| status | SINGLETON |
| subject.fl_str_mv | 3D-printed mesh meniscus Meniscus tissue engineering Silk Fibroin subcutaneous implantation Suturability Suture retention |
| title | Suturable regenerated silk fibroin scaffold reinforced with 3D-printed polycaprolactone mesh: biomechanical performance and subcutaneous implantation |
| title_full | Suturable regenerated silk fibroin scaffold reinforced with 3D-printed polycaprolactone mesh: biomechanical performance and subcutaneous implantation |
| title_fullStr | Suturable regenerated silk fibroin scaffold reinforced with 3D-printed polycaprolactone mesh: biomechanical performance and subcutaneous implantation |
| title_full_unstemmed | Suturable regenerated silk fibroin scaffold reinforced with 3D-printed polycaprolactone mesh: biomechanical performance and subcutaneous implantation |
| title_short | Suturable regenerated silk fibroin scaffold reinforced with 3D-printed polycaprolactone mesh: biomechanical performance and subcutaneous implantation |
| title_sort | Suturable regenerated silk fibroin scaffold reinforced with 3D-printed polycaprolactone mesh: biomechanical performance and subcutaneous implantation |
| topic | 3D-printed mesh meniscus Meniscus tissue engineering Silk Fibroin subcutaneous implantation Suturability Suture retention |
| topic_facet | 3D-printed mesh meniscus Meniscus tissue engineering Silk Fibroin subcutaneous implantation Suturability Suture retention |
| url | https://hdl.handle.net/1822/66387 |
| visible | 1 |