Publicação
Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications
| Resumo: | Cells microencapsulated in biocompatible semi-permeable polymeric membranes are effective as cell delivery systems while protecting the host against immune responses. In this study, cell encapsulation membranes were prepared based on carrageenan and alginate, two natural cationic polymers. Different formulations/conditions were explored to optimize the microcapsules which were characterized with respect to their morphology, mechanical stability, and cytotoxicity. Spherical-shaped microcapsules were obtained from all the polymeric systems. The iota-carrageenan/sodium alginate microcapsules exhibited the best stability and permeability, and therefore, these were selected for the cell encapsulation. These capsules provided an environment that supported cell proliferation and have the potential for tissue engineering as well as other cell-based therapy applications. |
|---|---|
| Autores principais: | Luna, S. M. |
| Outros Autores: | Gomes, Manuela E.; Mano, J. F.; Reis, R. L. |
| Assunto: | Capsules Carrageenan Alginate Fibroblasts Cell viability |
| Ano: | 2010 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867438689818247168 |
|---|---|
| author | Luna, S. M. |
| author2 | Gomes, Manuela E. Mano, J. F. Reis, R. L. |
| author2_role | author author author |
| author_facet | Luna, S. M. Gomes, Manuela E. Mano, J. F. Reis, R. L. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Luna, S. M.\"},{\"Person.name\":\"Gomes, Manuela E.\"},{\"Person.name\":\"Mano, J. F.\"},{\"Person.name\":\"Reis, R. L.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Luna, S. M. Gomes, Manuela E. Mano, J. F. Reis, R. L. |
| datacite.date.Accepted.fl_str_mv | 2010-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2012-10-26T16:14:49Z |
| datacite.date.embargoed.fl_str_mv | 2012-10-26T16:14:49Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Capsules Carrageenan Alginate Fibroblasts Cell viability |
| datacite.titles.title.fl_str_mv | Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Luna, S. M. Gomes, Manuela E. Mano, J. F. Reis, R. L. |
| dc.date.Accepted.fl_str_mv | 2010-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2012-10-26T16:14:49Z |
| dc.date.embargoed.fl_str_mv | 2012-10-26T16:14:49Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/20561 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | SAGE |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Capsules Carrageenan Alginate Fibroblasts Cell viability |
| dc.title.fl_str_mv | Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Cells microencapsulated in biocompatible semi-permeable polymeric membranes are effective as cell delivery systems while protecting the host against immune responses. In this study, cell encapsulation membranes were prepared based on carrageenan and alginate, two natural cationic polymers. Different formulations/conditions were explored to optimize the microcapsules which were characterized with respect to their morphology, mechanical stability, and cytotoxicity. Spherical-shaped microcapsules were obtained from all the polymeric systems. The iota-carrageenan/sodium alginate microcapsules exhibited the best stability and permeability, and therefore, these were selected for the cell encapsulation. These capsules provided an environment that supported cell proliferation and have the potential for tissue engineering as well as other cell-based therapy applications. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/58b2d262-e7dc-4a66-8bc0-6bff1ddde897/download |
| id | rum_c8fda8a74523c4fc1ba7f0b6c19f96be |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/20561 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/20561 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Luna, S. M. Gomes, Manuela E. Mano, J. F. Reis, R. L. |
| publishDate | 2010 |
| publisher.none.fl_str_mv | SAGE |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engSAGEporCells microencapsulated in biocompatible semi-permeable polymeric membranes are effective as cell delivery systems while protecting the host against immune responses. In this study, cell encapsulation membranes were prepared based on carrageenan and alginate, two natural cationic polymers. Different formulations/conditions were explored to optimize the microcapsules which were characterized with respect to their morphology, mechanical stability, and cytotoxicity. Spherical-shaped microcapsules were obtained from all the polymeric systems. The iota-carrageenan/sodium alginate microcapsules exhibited the best stability and permeability, and therefore, these were selected for the cell encapsulation. These capsules provided an environment that supported cell proliferation and have the potential for tissue engineering as well as other cell-based therapy applications.application/pdfporDevelopment of a novel cell encapsulation system based on natural origin polymers for tissue engineering applicationsLuna, S. M.Gomes, Manuela E.Mano, J. F.Reis, R. L.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf0883-9115ISSNIsPartOf1530-8030DOIIsPartOf10.1177/08839115103721732012-10-26T16:14:49Z20102010-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/20561http://purl.org/coar/access_right/c_abf2open accessCapsulesCarrageenanAlginateFibroblastsCell viability489523 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/58b2d262-e7dc-4a66-8bc0-6bff1ddde897/download |
| spellingShingle | Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications Luna, S. M. Capsules Carrageenan Alginate Fibroblasts Cell viability |
| status | SINGLETON |
| subject.fl_str_mv | Capsules Carrageenan Alginate Fibroblasts Cell viability |
| title | Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications |
| title_full | Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications |
| title_fullStr | Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications |
| title_full_unstemmed | Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications |
| title_short | Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications |
| title_sort | Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications |
| topic | Capsules Carrageenan Alginate Fibroblasts Cell viability |
| topic_facet | Capsules Carrageenan Alginate Fibroblasts Cell viability |
| url | https://hdl.handle.net/1822/20561 |
| visible | 1 |