Publicação
Brazilian red propolis: extracts production, physicochemical characterization, and cytotoxicity profile for antitumor activity
| Resumo: | Brazilian red propolis has been proposed as a new source of compounds with cytotoxic activity. Red propolis is a resinous material of vegetal origin, synthesized from the bees of the Appis mellifera family, with recognized biological properties. To obtain actives of low polarity and high cytotoxic profile from red propolis, in this work, we proposed a new solvent accelerated extraction method. A complete 23 factorial design was carried out to evaluate the influence of the independent variables or factors (e.g., temperature, number of cycles, and extraction time) on the dependent variable or response (i.e., yield of production). The extracts were analyzed by gas chromatography coupled with mass spectrometry for the identification of chemical compounds. Gas chromatography analysis revealed the presence of hydrocarbons, alcohols, ketones, ethers, and terpenes, such as lupeol, lupenone, and lupeol acetate, in most of the obtained extracts. To evaluate the cytotoxicity profile of the obtained bioactives, the 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyl-2-H-tetrazolium bromide colorimetric assay was performed in different tumor cell lines (HCT116 and PC3). The results show that the extract obtained from 70 °C and one cycle of extraction of 10 min exhibited the highest cytotoxic activity against the tested cell lines. The highest yield, however, did not indicate the highest cytotoxic activity, but the optimal extraction conditions were indeed dependent on the temperature (i.e., 70 °C). |
|---|---|
| Autores principais: | de Carvalho, Felipe Mendes de Andrade |
| Outros Autores: | Schneider, Jaderson Kleveston; de Jesus, Carla Viviane Freitas; de Andrade, Luciana Nalone; Amaral, Ricardo Guimarães; David, Jorge Maurício; Krause, Laíza Canielas; Severino, Patrícia; Soares, Cleide Mara Faria; Bastos, Elina Caramão; Padilha, Francine Ferreira; Gomes, Silvana Vieira Flores; Capasso, Raffaele; Santini, Antonello; Souto, Eliana B.; de Albuquerque-Júnior, Ricardo Luiz Cavalcanti |
| Assunto: | red propolis extraction supercritical liquids antitumor activity |
| Ano: | 2020 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866270717092298752 |
|---|---|
| author | de Carvalho, Felipe Mendes de Andrade |
| author2 | Schneider, Jaderson Kleveston de Jesus, Carla Viviane Freitas de Andrade, Luciana Nalone Amaral, Ricardo Guimarães David, Jorge Maurício Krause, Laíza Canielas Severino, Patrícia Soares, Cleide Mara Faria Bastos, Elina Caramão Padilha, Francine Ferreira Gomes, Silvana Vieira Flores Capasso, Raffaele Santini, Antonello Souto, Eliana B. de Albuquerque-Júnior, Ricardo Luiz Cavalcanti |
| author2_role | author author author author author author author author author author author author author author author |
| author_facet | de Carvalho, Felipe Mendes de Andrade Schneider, Jaderson Kleveston de Jesus, Carla Viviane Freitas de Andrade, Luciana Nalone Amaral, Ricardo Guimarães David, Jorge Maurício Krause, Laíza Canielas Severino, Patrícia Soares, Cleide Mara Faria Bastos, Elina Caramão Padilha, Francine Ferreira Gomes, Silvana Vieira Flores Capasso, Raffaele Santini, Antonello Souto, Eliana B. de Albuquerque-Júnior, Ricardo Luiz Cavalcanti |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"de Carvalho, Felipe Mendes de Andrade\"},{\"Person.name\":\"Schneider, Jaderson Kleveston\"},{\"Person.name\":\"de Jesus, Carla Viviane Freitas\"},{\"Person.name\":\"de Andrade, Luciana Nalone\"},{\"Person.name\":\"Amaral, Ricardo Guimarães\"},{\"Person.name\":\"David, Jorge Maurício\"},{\"Person.name\":\"Krause, Laíza Canielas\"},{\"Person.name\":\"Severino, Patrícia\"},{\"Person.name\":\"Soares, Cleide Mara Faria\"},{\"Person.name\":\"Bastos, Elina Caramão\"},{\"Person.name\":\"Padilha, Francine Ferreira\"},{\"Person.name\":\"Gomes, Silvana Vieira Flores\"},{\"Person.name\":\"Capasso, Raffaele\"},{\"Person.name\":\"Santini, Antonello\"},{\"Person.name\":\"Souto, Eliana B.\"},{\"Person.name\":\"de Albuquerque-Júnior, Ricardo Luiz Cavalcanti\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | de Carvalho, Felipe Mendes de Andrade Schneider, Jaderson Kleveston de Jesus, Carla Viviane Freitas de Andrade, Luciana Nalone Amaral, Ricardo Guimarães David, Jorge Maurício Krause, Laíza Canielas Severino, Patrícia Soares, Cleide Mara Faria Bastos, Elina Caramão Padilha, Francine Ferreira Gomes, Silvana Vieira Flores Capasso, Raffaele Santini, Antonello Souto, Eliana B. de Albuquerque-Júnior, Ricardo Luiz Cavalcanti |
| datacite.date.Accepted.fl_str_mv | 2020-05-06T00:00:00Z |
| datacite.date.available.fl_str_mv | 2020-05-09T15:09:32Z |
| datacite.date.embargoed.fl_str_mv | 2020-05-09T15:09:32Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | red propolis extraction supercritical liquids antitumor activity |
| datacite.titles.title.fl_str_mv | Brazilian red propolis: extracts production, physicochemical characterization, and cytotoxicity profile for antitumor activity |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | de Carvalho, Felipe Mendes de Andrade Schneider, Jaderson Kleveston de Jesus, Carla Viviane Freitas de Andrade, Luciana Nalone Amaral, Ricardo Guimarães David, Jorge Maurício Krause, Laíza Canielas Severino, Patrícia Soares, Cleide Mara Faria Bastos, Elina Caramão Padilha, Francine Ferreira Gomes, Silvana Vieira Flores Capasso, Raffaele Santini, Antonello Souto, Eliana B. de Albuquerque-Júnior, Ricardo Luiz Cavalcanti |
| dc.date.Accepted.fl_str_mv | 2020-05-06T00:00:00Z |
| dc.date.available.fl_str_mv | 2020-05-09T15:09:32Z |
| dc.date.embargoed.fl_str_mv | 2020-05-09T15:09:32Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/65301 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Multidisciplinary Digital Publishing Institute (MDPI) |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.rights.rights.copyright.fl_str_mv | openAccess |
| dc.subject.none.fl_str_mv | red propolis extraction supercritical liquids antitumor activity |
| dc.title.fl_str_mv | Brazilian red propolis: extracts production, physicochemical characterization, and cytotoxicity profile for antitumor activity |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Brazilian red propolis has been proposed as a new source of compounds with cytotoxic activity. Red propolis is a resinous material of vegetal origin, synthesized from the bees of the Appis mellifera family, with recognized biological properties. To obtain actives of low polarity and high cytotoxic profile from red propolis, in this work, we proposed a new solvent accelerated extraction method. A complete 23 factorial design was carried out to evaluate the influence of the independent variables or factors (e.g., temperature, number of cycles, and extraction time) on the dependent variable or response (i.e., yield of production). The extracts were analyzed by gas chromatography coupled with mass spectrometry for the identification of chemical compounds. Gas chromatography analysis revealed the presence of hydrocarbons, alcohols, ketones, ethers, and terpenes, such as lupeol, lupenone, and lupeol acetate, in most of the obtained extracts. To evaluate the cytotoxicity profile of the obtained bioactives, the 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyl-2-H-tetrazolium bromide colorimetric assay was performed in different tumor cell lines (HCT116 and PC3). The results show that the extract obtained from 70 °C and one cycle of extraction of 10 min exhibited the highest cytotoxic activity against the tested cell lines. The highest yield, however, did not indicate the highest cytotoxic activity, but the optimal extraction conditions were indeed dependent on the temperature (i.e., 70 °C). |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/1e284f73-ec1a-447e-bf72-5d411965fa1c/download |
| id | rum_c9b1e9c5fda2fe7c3558cc4fcf6b682d |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/65301 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/65301 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | de Carvalho, Felipe Mendes de Andrade Schneider, Jaderson Kleveston de Jesus, Carla Viviane Freitas de Andrade, Luciana Nalone Amaral, Ricardo Guimarães David, Jorge Maurício Krause, Laíza Canielas Severino, Patrícia Soares, Cleide Mara Faria Bastos, Elina Caramão Padilha, Francine Ferreira Gomes, Silvana Vieira Flores Capasso, Raffaele Santini, Antonello Souto, Eliana B. de Albuquerque-Júnior, Ricardo Luiz Cavalcanti |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Multidisciplinary Digital Publishing Institute (MDPI) |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engMultidisciplinary Digital Publishing Institute (MDPI)porBrazilian red propolis has been proposed as a new source of compounds with cytotoxic activity. Red propolis is a resinous material of vegetal origin, synthesized from the bees of the Appis mellifera family, with recognized biological properties. To obtain actives of low polarity and high cytotoxic profile from red propolis, in this work, we proposed a new solvent accelerated extraction method. A complete 23 factorial design was carried out to evaluate the influence of the independent variables or factors (e.g., temperature, number of cycles, and extraction time) on the dependent variable or response (i.e., yield of production). The extracts were analyzed by gas chromatography coupled with mass spectrometry for the identification of chemical compounds. Gas chromatography analysis revealed the presence of hydrocarbons, alcohols, ketones, ethers, and terpenes, such as lupeol, lupenone, and lupeol acetate, in most of the obtained extracts. To evaluate the cytotoxicity profile of the obtained bioactives, the 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyl-2-H-tetrazolium bromide colorimetric assay was performed in different tumor cell lines (HCT116 and PC3). The results show that the extract obtained from 70 °C and one cycle of extraction of 10 min exhibited the highest cytotoxic activity against the tested cell lines. The highest yield, however, did not indicate the highest cytotoxic activity, but the optimal extraction conditions were indeed dependent on the temperature (i.e., 70 °C).application/pdfporBrazilian red propolis: extracts production, physicochemical characterization, and cytotoxicity profile for antitumor activityde Carvalho, Felipe Mendes de AndradeSchneider, Jaderson Klevestonde Jesus, Carla Viviane Freitasde Andrade, Luciana NaloneAmaral, Ricardo GuimarãesDavid, Jorge MaurícioKrause, Laíza CanielasSeverino, PatríciaSoares, Cleide Mara FariaBastos, Elina CaramãoPadilha, Francine FerreiraGomes, Silvana Vieira FloresCapasso, RaffaeleSantini, AntonelloSouto, Eliana B.de Albuquerque-Júnior, Ricardo Luiz CavalcantiHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptDOIIsPartOf10.3390/biom100507262020-05-09T15:09:32Z2020-05-062020-05-09T13:33:05Z2020-05-06T00:00:00ZHandlehttps://hdl.handle.net/1822/65301http://purl.org/coar/access_right/c_abf2open accessred propolisextractionsupercritical liquidsantitumor activity1022176 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2020-05-06http://creativecommons.org/licenses/by/4.0/openAccesshttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/1e284f73-ec1a-447e-bf72-5d411965fa1c/download |
| spellingShingle | Brazilian red propolis: extracts production, physicochemical characterization, and cytotoxicity profile for antitumor activity de Carvalho, Felipe Mendes de Andrade red propolis extraction supercritical liquids antitumor activity |
| status | SINGLETON |
| subject.fl_str_mv | red propolis extraction supercritical liquids antitumor activity |
| title | Brazilian red propolis: extracts production, physicochemical characterization, and cytotoxicity profile for antitumor activity |
| title_full | Brazilian red propolis: extracts production, physicochemical characterization, and cytotoxicity profile for antitumor activity |
| title_fullStr | Brazilian red propolis: extracts production, physicochemical characterization, and cytotoxicity profile for antitumor activity |
| title_full_unstemmed | Brazilian red propolis: extracts production, physicochemical characterization, and cytotoxicity profile for antitumor activity |
| title_short | Brazilian red propolis: extracts production, physicochemical characterization, and cytotoxicity profile for antitumor activity |
| title_sort | Brazilian red propolis: extracts production, physicochemical characterization, and cytotoxicity profile for antitumor activity |
| topic | red propolis extraction supercritical liquids antitumor activity |
| topic_facet | red propolis extraction supercritical liquids antitumor activity |
| url | https://hdl.handle.net/1822/65301 |
| visible | 1 |