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Brazilian red propolis: extracts production, physicochemical characterization, and cytotoxicity profile for antitumor activity

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Resumo:Brazilian red propolis has been proposed as a new source of compounds with cytotoxic activity. Red propolis is a resinous material of vegetal origin, synthesized from the bees of the Appis mellifera family, with recognized biological properties. To obtain actives of low polarity and high cytotoxic profile from red propolis, in this work, we proposed a new solvent accelerated extraction method. A complete 23 factorial design was carried out to evaluate the influence of the independent variables or factors (e.g., temperature, number of cycles, and extraction time) on the dependent variable or response (i.e., yield of production). The extracts were analyzed by gas chromatography coupled with mass spectrometry for the identification of chemical compounds. Gas chromatography analysis revealed the presence of hydrocarbons, alcohols, ketones, ethers, and terpenes, such as lupeol, lupenone, and lupeol acetate, in most of the obtained extracts. To evaluate the cytotoxicity profile of the obtained bioactives, the 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyl-2-H-tetrazolium bromide colorimetric assay was performed in different tumor cell lines (HCT116 and PC3). The results show that the extract obtained from 70 °C and one cycle of extraction of 10 min exhibited the highest cytotoxic activity against the tested cell lines. The highest yield, however, did not indicate the highest cytotoxic activity, but the optimal extraction conditions were indeed dependent on the temperature (i.e., 70 °C).
Autores principais:de Carvalho, Felipe Mendes de Andrade
Outros Autores:Schneider, Jaderson Kleveston; de Jesus, Carla Viviane Freitas; de Andrade, Luciana Nalone; Amaral, Ricardo Guimarães; David, Jorge Maurício; Krause, Laíza Canielas; Severino, Patrícia; Soares, Cleide Mara Faria; Bastos, Elina Caramão; Padilha, Francine Ferreira; Gomes, Silvana Vieira Flores; Capasso, Raffaele; Santini, Antonello; Souto, Eliana B.; de Albuquerque-Júnior, Ricardo Luiz Cavalcanti
Assunto:red propolis extraction supercritical liquids antitumor activity
Ano:2020
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho

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