Publicação
Polydopamine-mediated immobilization of alginate lyase to prevent P. aeruginosa adhesion
| Resumo: | Given alginate’s contribution to Pseudomonas aeruginosa virulence, it has long been considered a promising target for interventional therapies, which have been performed by using the enzyme alginate lyase. In this work, instead of treating pre-established mucoid biofi lms, alginate lyase is immobilized onto a surface as a preventive measure against P. aeruginosa adhesion. A polydopamine dip-coating strategy is employed for functionalization of polycarbonate surfaces. Enzyme immobilization is confi rmed by surface characterization. Surfaces functionalized with alginate lyase exhibit anti-adhesive properties, inhibiting the attachment of the mucoid strain. Moreover, surfaces modifi ed with this enzyme also inhibit the adhesion of the tested non-mucoid strain. Unexpectedly, treatment with heat-inactivated enzyme also inhibits the attachment of mucoid and non-mucoid P. aeruginosa strains. These fi ndings suggest that the antibacterial performance of alginate lyase functional coatings is catalysis-independent, highlighting the importance of further studies to better understand its mechanism of action against P. aeruginosa strains. |
|---|---|
| Autores principais: | Alves, D. |
| Outros Autores: | Sileika, Tadas; Messersmith, Phillip; Pereira, Maria Olívia |
| Assunto: | Funcionalization of polycarbonate surfaces with alginate lyase Dopamine-based coating strategy Antiadhesion surfaces Catalysis-independent performance alginate lyase antibacterial coating catalysis-independent dopamine chemistry |
| Ano: | 2016 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866875867345453056 |
|---|---|
| author | Alves, D. |
| author2 | Sileika, Tadas Messersmith, Phillip Pereira, Maria Olívia |
| author2_role | author author author |
| author_facet | Alves, D. Sileika, Tadas Messersmith, Phillip Pereira, Maria Olívia |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Alves, D.\"},{\"Person.name\":\"Sileika, Tadas\"},{\"Person.name\":\"Messersmith, Phillip\"},{\"Person.name\":\"Pereira, Maria Olívia\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Alves, D. Sileika, Tadas Messersmith, Phillip Pereira, Maria Olívia |
| datacite.date.Accepted.fl_str_mv | 2016-09-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2016-09-12T10:30:58Z |
| datacite.date.embargoed.fl_str_mv | 2016-09-12T10:30:58Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Funcionalization of polycarbonate surfaces with alginate lyase Dopamine-based coating strategy Antiadhesion surfaces Catalysis-independent performance alginate lyase antibacterial coating catalysis-independent dopamine chemistry |
| datacite.titles.title.fl_str_mv | Polydopamine-mediated immobilization of alginate lyase to prevent P. aeruginosa adhesion |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Alves, D. Sileika, Tadas Messersmith, Phillip Pereira, Maria Olívia |
| dc.date.Accepted.fl_str_mv | 2016-09-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2016-09-12T10:30:58Z |
| dc.date.embargoed.fl_str_mv | 2016-09-12T10:30:58Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/42544 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | John Wiley and Sons |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Funcionalization of polycarbonate surfaces with alginate lyase Dopamine-based coating strategy Antiadhesion surfaces Catalysis-independent performance alginate lyase antibacterial coating catalysis-independent dopamine chemistry |
| dc.title.fl_str_mv | Polydopamine-mediated immobilization of alginate lyase to prevent P. aeruginosa adhesion |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Given alginate’s contribution to Pseudomonas aeruginosa virulence, it has long been considered a promising target for interventional therapies, which have been performed by using the enzyme alginate lyase. In this work, instead of treating pre-established mucoid biofi lms, alginate lyase is immobilized onto a surface as a preventive measure against P. aeruginosa adhesion. A polydopamine dip-coating strategy is employed for functionalization of polycarbonate surfaces. Enzyme immobilization is confi rmed by surface characterization. Surfaces functionalized with alginate lyase exhibit anti-adhesive properties, inhibiting the attachment of the mucoid strain. Moreover, surfaces modifi ed with this enzyme also inhibit the adhesion of the tested non-mucoid strain. Unexpectedly, treatment with heat-inactivated enzyme also inhibits the attachment of mucoid and non-mucoid P. aeruginosa strains. These fi ndings suggest that the antibacterial performance of alginate lyase functional coatings is catalysis-independent, highlighting the importance of further studies to better understand its mechanism of action against P. aeruginosa strains. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/aa8b5280-3842-4666-adad-7e97db2514aa/download |
| id | rum_cdec033656a2b944bfbc6ffe013d7de2 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/42544 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/42544 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Alves, D. Sileika, Tadas Messersmith, Phillip Pereira, Maria Olívia |
| publishDate | 2016 |
| publisher.none.fl_str_mv | John Wiley and Sons |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engJohn Wiley and SonsporGiven alginate’s contribution to Pseudomonas aeruginosa virulence, it has long been considered a promising target for interventional therapies, which have been performed by using the enzyme alginate lyase. In this work, instead of treating pre-established mucoid biofi lms, alginate lyase is immobilized onto a surface as a preventive measure against P. aeruginosa adhesion. A polydopamine dip-coating strategy is employed for functionalization of polycarbonate surfaces. Enzyme immobilization is confi rmed by surface characterization. Surfaces functionalized with alginate lyase exhibit anti-adhesive properties, inhibiting the attachment of the mucoid strain. Moreover, surfaces modifi ed with this enzyme also inhibit the adhesion of the tested non-mucoid strain. Unexpectedly, treatment with heat-inactivated enzyme also inhibits the attachment of mucoid and non-mucoid P. aeruginosa strains. These fi ndings suggest that the antibacterial performance of alginate lyase functional coatings is catalysis-independent, highlighting the importance of further studies to better understand its mechanism of action against P. aeruginosa strains.application/pdfporPolydopamine-mediated immobilization of alginate lyase to prevent P. aeruginosa adhesionAlves, D.Sileika, TadasMessersmith, PhillipPereira, Maria OlíviaHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf1616-5187DOIIsPartOf10.1002/mabi.2016000772016-09-12T10:30:58Z2016-092016-09-10T21:08:45Z2016-09-01T00:00:00ZHandlehttps://hdl.handle.net/1822/42544http://purl.org/coar/access_right/c_abf2open accessFuncionalization of polycarbonate surfaces with alginate lyaseDopamine-based coating strategyAntiadhesion surfacesCatalysis-independent performancealginate lyaseantibacterial coatingcatalysis-independentdopamine chemistry5483868 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/aa8b5280-3842-4666-adad-7e97db2514aa/download |
| spellingShingle | Polydopamine-mediated immobilization of alginate lyase to prevent P. aeruginosa adhesion Alves, D. Funcionalization of polycarbonate surfaces with alginate lyase Dopamine-based coating strategy Antiadhesion surfaces Catalysis-independent performance alginate lyase antibacterial coating catalysis-independent dopamine chemistry |
| status | SINGLETON |
| subject.fl_str_mv | Funcionalization of polycarbonate surfaces with alginate lyase Dopamine-based coating strategy Antiadhesion surfaces Catalysis-independent performance alginate lyase antibacterial coating catalysis-independent dopamine chemistry |
| title | Polydopamine-mediated immobilization of alginate lyase to prevent P. aeruginosa adhesion |
| title_full | Polydopamine-mediated immobilization of alginate lyase to prevent P. aeruginosa adhesion |
| title_fullStr | Polydopamine-mediated immobilization of alginate lyase to prevent P. aeruginosa adhesion |
| title_full_unstemmed | Polydopamine-mediated immobilization of alginate lyase to prevent P. aeruginosa adhesion |
| title_short | Polydopamine-mediated immobilization of alginate lyase to prevent P. aeruginosa adhesion |
| title_sort | Polydopamine-mediated immobilization of alginate lyase to prevent P. aeruginosa adhesion |
| topic | Funcionalization of polycarbonate surfaces with alginate lyase Dopamine-based coating strategy Antiadhesion surfaces Catalysis-independent performance alginate lyase antibacterial coating catalysis-independent dopamine chemistry |
| topic_facet | Funcionalization of polycarbonate surfaces with alginate lyase Dopamine-based coating strategy Antiadhesion surfaces Catalysis-independent performance alginate lyase antibacterial coating catalysis-independent dopamine chemistry |
| url | https://hdl.handle.net/1822/42544 |
| visible | 1 |