Publicação
Pulsed electric field reduces the permeability of potato cell wall
| Resumo: | The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FM1-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases. |
|---|---|
| Autores principais: | Gómez Galindo, Federico |
| Outros Autores: | Vernier, P. Thomas; Dejmek, Petr; Vicente, A. A.; Gundersen, Martin A. |
| Assunto: | Cell wall FM1-43 eEectric fields Sress Oxidative cross-linking electric fields stress |
| Ano: | 2008 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439288704040960 |
|---|---|
| author | Gómez Galindo, Federico |
| author2 | Vernier, P. Thomas Dejmek, Petr Vicente, A. A. Gundersen, Martin A. |
| author2_role | author author author author |
| author_facet | Gómez Galindo, Federico Vernier, P. Thomas Dejmek, Petr Vicente, A. A. Gundersen, Martin A. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Gómez Galindo, Federico\"},{\"Person.name\":\"Vernier, P. Thomas\"},{\"Person.name\":\"Dejmek, Petr\"},{\"Person.name\":\"Vicente, A. A.\"},{\"Person.name\":\"Gundersen, Martin A.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Gómez Galindo, Federico Vernier, P. Thomas Dejmek, Petr Vicente, A. A. Gundersen, Martin A. |
| datacite.date.Accepted.fl_str_mv | 2008-05-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2010-02-19T15:56:03Z |
| datacite.date.embargoed.fl_str_mv | 2010-02-19T15:56:03Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Cell wall FM1-43 eEectric fields Sress Oxidative cross-linking electric fields stress |
| datacite.titles.title.fl_str_mv | Pulsed electric field reduces the permeability of potato cell wall |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Gómez Galindo, Federico Vernier, P. Thomas Dejmek, Petr Vicente, A. A. Gundersen, Martin A. |
| dc.date.Accepted.fl_str_mv | 2008-05-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2010-02-19T15:56:03Z |
| dc.date.embargoed.fl_str_mv | 2010-02-19T15:56:03Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/10424 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | John Wiley and Sons |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Cell wall FM1-43 eEectric fields Sress Oxidative cross-linking electric fields stress |
| dc.title.fl_str_mv | Pulsed electric field reduces the permeability of potato cell wall |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FM1-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/9a0171a5-f4e2-4d33-bbd5-f23e2181bede/download |
| id | rum_cf2fbf672240abe2c835534fe8e1ca39 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/10424 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/10424 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Gómez Galindo, Federico Vernier, P. Thomas Dejmek, Petr Vicente, A. A. Gundersen, Martin A. |
| publishDate | 2008 |
| publisher.none.fl_str_mv | John Wiley and Sons |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engJohn Wiley and SonsporThe effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FM1-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases.application/pdfporPulsed electric field reduces the permeability of potato cell wallGómez Galindo, FedericoVernier, P. ThomasDejmek, PetrVicente, A. A.Gundersen, Martin A.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATION"Bioelectromagnetic". ISSN 0197-8462. 29:4 (May 2008) 296-301.PMID18163439ISSNIsPartOf0197-8462DOIIsPartOf10.1002/bem.203942010-02-19T15:56:03Z2008-052008-05-01T00:00:00ZHandlehttps://hdl.handle.net/1822/10424http://purl.org/coar/access_right/c_abf2open accessCell wallFM1-43eEectric fieldsSressOxidative cross-linkingelectric fieldsstress156416 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/9a0171a5-f4e2-4d33-bbd5-f23e2181bede/download |
| spellingShingle | Pulsed electric field reduces the permeability of potato cell wall Gómez Galindo, Federico Cell wall FM1-43 eEectric fields Sress Oxidative cross-linking electric fields stress |
| status | SINGLETON |
| subject.fl_str_mv | Cell wall FM1-43 eEectric fields Sress Oxidative cross-linking electric fields stress |
| title | Pulsed electric field reduces the permeability of potato cell wall |
| title_full | Pulsed electric field reduces the permeability of potato cell wall |
| title_fullStr | Pulsed electric field reduces the permeability of potato cell wall |
| title_full_unstemmed | Pulsed electric field reduces the permeability of potato cell wall |
| title_short | Pulsed electric field reduces the permeability of potato cell wall |
| title_sort | Pulsed electric field reduces the permeability of potato cell wall |
| topic | Cell wall FM1-43 eEectric fields Sress Oxidative cross-linking electric fields stress |
| topic_facet | Cell wall FM1-43 eEectric fields Sress Oxidative cross-linking electric fields stress |
| url | https://hdl.handle.net/1822/10424 |
| visible | 1 |