Publicação
Anaerobic biodegradation of oleic and palmitic acids : evidence of mass transfer limitations caused by long chain fatty acid accumulation onto the anaerobic sludge
| Resumo: | Palmitic acid was the main long chain fatty acids (LCFA) that accumulated onto the anaerobic sludge when oleic acid was fed to an EGSB reactor. The conversion between oleic and palmitic acid was linked to the biological activity. When palmitic acid was fed to an EGSB reactor it represented also the main LCFA that accumulated onto the sludge. The way of palmitic acid accumulation was different in the oleic and in the palmitic acid fed reactors.Whenoleic acid was fed, the biomass-associated LCFA (83% as palmitic acid) were mainly adsorbed and entrapped in the sludge that became ‘‘encapsulated’’ by an LCFA layer. However, when palmitic acid was fed, the biomass-associated LCFA (the totality as palmitic acid) was mainly precipitated in white spots like precipitates in between the sludge, which remained ‘‘non-encapsulated.’’ The two sludges were compared in terms of the specific methanogenic activity (SMA) in the presence of acetate, propionate, butyrate, and H2CO2, before and after the mineralization of similar amounts of biomassassociated LCFA (4.6 and 5.2 g COD-LCFA/g of volatile suspended solids (VSS), for the oleic and palmitic acid fed sludge, respectively). The ‘‘non-encapsulated,’’ sludge exhibited a considerable initial methanogenic activity on all the tested substrates, with the single exception of butyrate. However, with the ‘‘encapsulated’’ sludge only methane production from ethanol andH2/CO2 was detected, after a lag phase of about 50 h. After mineralization of the biomass-associated LCFA, both sludges exhibited activities of similar order of magnitude in the presence of the same individual substrates and significantly higher than before. The results evidenced that LCFA accumulation onto the sludge can create a physical barrier and hinder the transfer of substrates and products, inducing a delay on the initial methane production. Whatever the mechanism, metabolic or physical, that is behind this inhibition, it is reversible, being eliminated after the depletion of the biomass-associated LCFA. |
|---|---|
| Autores principais: | Pereira, M. A. |
| Outros Autores: | Pires, O. C.; Mota, M.; Alves, M. M. |
| Assunto: | LCFA Methanogenic activity Transport limitations |
| Ano: | 2005 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439706923335680 |
|---|---|
| author | Pereira, M. A. |
| author2 | Pires, O. C. Mota, M. Alves, M. M. |
| author2_role | author author author |
| author_facet | Pereira, M. A. Pires, O. C. Mota, M. Alves, M. M. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Pereira, M. A.\"},{\"Person.name\":\"Pires, O. C.\"},{\"Person.name\":\"Mota, M.\"},{\"Person.name\":\"Alves, M. M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Pereira, M. A. Pires, O. C. Mota, M. Alves, M. M. |
| datacite.date.Accepted.fl_str_mv | 2005-10-05T00:00:00Z |
| datacite.date.available.fl_str_mv | 2005-11-14T17:44:04Z |
| datacite.date.embargoed.fl_str_mv | 2005-11-14T17:44:04Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | LCFA Methanogenic activity Transport limitations |
| datacite.titles.title.fl_str_mv | Anaerobic biodegradation of oleic and palmitic acids : evidence of mass transfer limitations caused by long chain fatty acid accumulation onto the anaerobic sludge |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Pereira, M. A. Pires, O. C. Mota, M. Alves, M. M. |
| dc.date.Accepted.fl_str_mv | 2005-10-05T00:00:00Z |
| dc.date.available.fl_str_mv | 2005-11-14T17:44:04Z |
| dc.date.embargoed.fl_str_mv | 2005-11-14T17:44:04Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/3488 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Wiley |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | LCFA Methanogenic activity Transport limitations |
| dc.title.fl_str_mv | Anaerobic biodegradation of oleic and palmitic acids : evidence of mass transfer limitations caused by long chain fatty acid accumulation onto the anaerobic sludge |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Palmitic acid was the main long chain fatty acids (LCFA) that accumulated onto the anaerobic sludge when oleic acid was fed to an EGSB reactor. The conversion between oleic and palmitic acid was linked to the biological activity. When palmitic acid was fed to an EGSB reactor it represented also the main LCFA that accumulated onto the sludge. The way of palmitic acid accumulation was different in the oleic and in the palmitic acid fed reactors.Whenoleic acid was fed, the biomass-associated LCFA (83% as palmitic acid) were mainly adsorbed and entrapped in the sludge that became ‘‘encapsulated’’ by an LCFA layer. However, when palmitic acid was fed, the biomass-associated LCFA (the totality as palmitic acid) was mainly precipitated in white spots like precipitates in between the sludge, which remained ‘‘non-encapsulated.’’ The two sludges were compared in terms of the specific methanogenic activity (SMA) in the presence of acetate, propionate, butyrate, and H2CO2, before and after the mineralization of similar amounts of biomassassociated LCFA (4.6 and 5.2 g COD-LCFA/g of volatile suspended solids (VSS), for the oleic and palmitic acid fed sludge, respectively). The ‘‘non-encapsulated,’’ sludge exhibited a considerable initial methanogenic activity on all the tested substrates, with the single exception of butyrate. However, with the ‘‘encapsulated’’ sludge only methane production from ethanol andH2/CO2 was detected, after a lag phase of about 50 h. After mineralization of the biomass-associated LCFA, both sludges exhibited activities of similar order of magnitude in the presence of the same individual substrates and significantly higher than before. The results evidenced that LCFA accumulation onto the sludge can create a physical barrier and hinder the transfer of substrates and products, inducing a delay on the initial methane production. Whatever the mechanism, metabolic or physical, that is behind this inhibition, it is reversible, being eliminated after the depletion of the biomass-associated LCFA. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/bd0a30ff-83f4-47ba-b0dd-4ebd87e54f3c/download |
| id | rum_cfc4c7a6958f72fdcef20f4e9db0bc50 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/3488 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/3488 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Pereira, M. A. Pires, O. C. Mota, M. Alves, M. M. |
| publishDate | 2005 |
| publisher.none.fl_str_mv | Wiley |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engWileyengPalmitic acid was the main long chain fatty acids (LCFA) that accumulated onto the anaerobic sludge when oleic acid was fed to an EGSB reactor. The conversion between oleic and palmitic acid was linked to the biological activity. When palmitic acid was fed to an EGSB reactor it represented also the main LCFA that accumulated onto the sludge. The way of palmitic acid accumulation was different in the oleic and in the palmitic acid fed reactors.Whenoleic acid was fed, the biomass-associated LCFA (83% as palmitic acid) were mainly adsorbed and entrapped in the sludge that became ‘‘encapsulated’’ by an LCFA layer. However, when palmitic acid was fed, the biomass-associated LCFA (the totality as palmitic acid) was mainly precipitated in white spots like precipitates in between the sludge, which remained ‘‘non-encapsulated.’’ The two sludges were compared in terms of the specific methanogenic activity (SMA) in the presence of acetate, propionate, butyrate, and H2CO2, before and after the mineralization of similar amounts of biomassassociated LCFA (4.6 and 5.2 g COD-LCFA/g of volatile suspended solids (VSS), for the oleic and palmitic acid fed sludge, respectively). The ‘‘non-encapsulated,’’ sludge exhibited a considerable initial methanogenic activity on all the tested substrates, with the single exception of butyrate. However, with the ‘‘encapsulated’’ sludge only methane production from ethanol andH2/CO2 was detected, after a lag phase of about 50 h. After mineralization of the biomass-associated LCFA, both sludges exhibited activities of similar order of magnitude in the presence of the same individual substrates and significantly higher than before. The results evidenced that LCFA accumulation onto the sludge can create a physical barrier and hinder the transfer of substrates and products, inducing a delay on the initial methane production. Whatever the mechanism, metabolic or physical, that is behind this inhibition, it is reversible, being eliminated after the depletion of the biomass-associated LCFA.application/pdfengAnaerobic biodegradation of oleic and palmitic acids : evidence of mass transfer limitations caused by long chain fatty acid accumulation onto the anaerobic sludgePereira, M. A.Pires, O. C.Mota, M.Alves, M. M.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATION"Biotechnology and bioengineering". ISSN 0006-3592. 92:1 (Oct. 2005) 15-23.PMID16136588ISSNIsPartOf0006-3592DOIIsPartOf10.1002/bit.205482005-11-14T17:44:04Z2005-10-052005-10-05T00:00:00ZHandlehttps://hdl.handle.net/1822/3488http://purl.org/coar/access_right/c_abf2open accessLCFAMethanogenic activityTransport limitations228820 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/bd0a30ff-83f4-47ba-b0dd-4ebd87e54f3c/download |
| spellingShingle | Anaerobic biodegradation of oleic and palmitic acids : evidence of mass transfer limitations caused by long chain fatty acid accumulation onto the anaerobic sludge Pereira, M. A. LCFA Methanogenic activity Transport limitations |
| status | SINGLETON |
| subject.fl_str_mv | LCFA Methanogenic activity Transport limitations |
| title | Anaerobic biodegradation of oleic and palmitic acids : evidence of mass transfer limitations caused by long chain fatty acid accumulation onto the anaerobic sludge |
| title_full | Anaerobic biodegradation of oleic and palmitic acids : evidence of mass transfer limitations caused by long chain fatty acid accumulation onto the anaerobic sludge |
| title_fullStr | Anaerobic biodegradation of oleic and palmitic acids : evidence of mass transfer limitations caused by long chain fatty acid accumulation onto the anaerobic sludge |
| title_full_unstemmed | Anaerobic biodegradation of oleic and palmitic acids : evidence of mass transfer limitations caused by long chain fatty acid accumulation onto the anaerobic sludge |
| title_short | Anaerobic biodegradation of oleic and palmitic acids : evidence of mass transfer limitations caused by long chain fatty acid accumulation onto the anaerobic sludge |
| title_sort | Anaerobic biodegradation of oleic and palmitic acids : evidence of mass transfer limitations caused by long chain fatty acid accumulation onto the anaerobic sludge |
| topic | LCFA Methanogenic activity Transport limitations |
| topic_facet | LCFA Methanogenic activity Transport limitations |
| url | https://hdl.handle.net/1822/3488 |
| visible | 1 |