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Penicillium tunisiense sp. nov., a novel species of Penicillium section Ramosa discovered from Tunisian orchard apples

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Resumo:Two similar Penicillium isolates could not be identified as previously described species in a survey of orchard apples from Tunisia for patulin-producing fungi. These isolates are described as novel species using multilocus DNA sequence analysis of partial -tubulin, calmodulin and nuclear ribosomal internal transcribed spacer regions; and morphological, physiological and biochemical characteristics. The isolates were considered negative for patulin production since the IDH gene fragment was not detected and the compound detected at the same retention time of patulin (14.9min) showed a different UV spectrum using U-HPLC/UV-DAD. In terms of phylogeny, the two isolates clustered with Penicillium section Ramosa and are closely related to Penicillium chroogomphum, Penicillium lenticrescens and Penicillium soppii. Furthermore, their macro- and micromorphological traits differed from these species. Hence, the isolates represent a novel species in Penicillium section Ramosa and the name Penicillium tunisiense sp. nov. is proposed, with the type strain MUM 17.62T (=ITEM 17445T).
Autores principais:Ouhibi, Salma
Outros Autores:Santos, Carla; Ghali, Ridha; Soares, Célia; Hedhili, Abderrazek; Paterson, R. R. M.; Lima, Nelson
Assunto:MLSA phylogeny calmodulin taxonomy ITS region ß-tubulin β-tubulin
Ano:2018
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho
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author Ouhibi, Salma
author2 Santos, Carla
Ghali, Ridha
Soares, Célia
Hedhili, Abderrazek
Paterson, R. R. M.
Lima, Nelson
author2_role author
author
author
author
author
author
author_facet Ouhibi, Salma
Santos, Carla
Ghali, Ridha
Soares, Célia
Hedhili, Abderrazek
Paterson, R. R. M.
Lima, Nelson
author_role author
contributor_name_str_mv Universidade do Minho
country_str PT
creators_json_txt [{\"Person.name\":\"Ouhibi, Salma\"},{\"Person.name\":\"Santos, Carla\"},{\"Person.name\":\"Ghali, Ridha\"},{\"Person.name\":\"Soares, Célia\"},{\"Person.name\":\"Hedhili, Abderrazek\"},{\"Person.name\":\"Paterson, R. R. M.\"},{\"Person.name\":\"Lima, Nelson\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Universidade do Minho
datacite.creators.creator.creatorName.fl_str_mv Ouhibi, Salma
Santos, Carla
Ghali, Ridha
Soares, Célia
Hedhili, Abderrazek
Paterson, R. R. M.
Lima, Nelson
datacite.date.Accepted.fl_str_mv 2018-08-20T00:00:00Z
datacite.date.available.fl_str_mv 2019-10-08T13:10:01Z
datacite.date.embargoed.fl_str_mv 2019-10-08T13:10:01Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv MLSA
phylogeny
calmodulin
taxonomy
ITS region
ß-tubulin
β-tubulin
datacite.titles.title.fl_str_mv Penicillium tunisiense sp. nov., a novel species of Penicillium section Ramosa discovered from Tunisian orchard apples
dc.contributor.none.fl_str_mv Universidade do Minho
dc.creator.none.fl_str_mv Ouhibi, Salma
Santos, Carla
Ghali, Ridha
Soares, Célia
Hedhili, Abderrazek
Paterson, R. R. M.
Lima, Nelson
dc.date.Accepted.fl_str_mv 2018-08-20T00:00:00Z
dc.date.available.fl_str_mv 2019-10-08T13:10:01Z
dc.date.embargoed.fl_str_mv 2019-10-08T13:10:01Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv https://hdl.handle.net/1822/61669
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv Society for General Microbiology
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv MLSA
phylogeny
calmodulin
taxonomy
ITS region
ß-tubulin
β-tubulin
dc.title.fl_str_mv Penicillium tunisiense sp. nov., a novel species of Penicillium section Ramosa discovered from Tunisian orchard apples
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description Two similar Penicillium isolates could not be identified as previously described species in a survey of orchard apples from Tunisia for patulin-producing fungi. These isolates are described as novel species using multilocus DNA sequence analysis of partial -tubulin, calmodulin and nuclear ribosomal internal transcribed spacer regions; and morphological, physiological and biochemical characteristics. The isolates were considered negative for patulin production since the IDH gene fragment was not detected and the compound detected at the same retention time of patulin (14.9min) showed a different UV spectrum using U-HPLC/UV-DAD. In terms of phylogeny, the two isolates clustered with Penicillium section Ramosa and are closely related to Penicillium chroogomphum, Penicillium lenticrescens and Penicillium soppii. Furthermore, their macro- and micromorphological traits differed from these species. Hence, the isolates represent a novel species in Penicillium section Ramosa and the name Penicillium tunisiense sp. nov. is proposed, with the type strain MUM 17.62T (=ITEM 17445T).
dirty 0
eu_rights_str_mv openAccess
format article
fulltext.url.fl_str_mv https://prod-dspace.uminho.pt/bitstreams/62c8c006-827d-4b4e-8255-aee7b42c9e56/download
id rum_d4f22c3fd218bda75112ea5d1aeffb60
identifier.url.fl_str_mv https://hdl.handle.net/1822/61669
instacron_str repositorium
institution Universidade do Minho
instname_str Universidade do Minho
language eng
network_acronym_str rum
network_name_str RepositóriUM - Universidade do Minho
oai_identifier_str oai:repositorium.uminho.pt:1822/61669
organization_str_mv urn:organizationAcronym:repositorium
person_str_mv Ouhibi, Salma
Santos, Carla
Ghali, Ridha
Soares, Célia
Hedhili, Abderrazek
Paterson, R. R. M.
Lima, Nelson
publishDate 2018
publisher.none.fl_str_mv Society for General Microbiology
reponame_str RepositóriUM - Universidade do Minho
repository_id_str urn:repositoryAcronym:rum
service_str_mv urn:repositoryAcronym:rum
spelling engSociety for General MicrobiologyporTwo similar Penicillium isolates could not be identified as previously described species in a survey of orchard apples from Tunisia for patulin-producing fungi. These isolates are described as novel species using multilocus DNA sequence analysis of partial -tubulin, calmodulin and nuclear ribosomal internal transcribed spacer regions; and morphological, physiological and biochemical characteristics. The isolates were considered negative for patulin production since the IDH gene fragment was not detected and the compound detected at the same retention time of patulin (14.9min) showed a different UV spectrum using U-HPLC/UV-DAD. In terms of phylogeny, the two isolates clustered with Penicillium section Ramosa and are closely related to Penicillium chroogomphum, Penicillium lenticrescens and Penicillium soppii. Furthermore, their macro- and micromorphological traits differed from these species. Hence, the isolates represent a novel species in Penicillium section Ramosa and the name Penicillium tunisiense sp. nov. is proposed, with the type strain MUM 17.62T (=ITEM 17445T).application/pdfporPenicillium tunisiense sp. nov., a novel species of Penicillium section Ramosa discovered from Tunisian orchard applesOuhibi, SalmaSantos, CarlaGhali, RidhaSoares, CéliaHedhili, AbderrazekPaterson, R. R. M.Lima, NelsonHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf1466-5026DOIIsPartOf10.1099/ijsem.0.0029622019-10-08T13:10:01Z2018-08-202019-09-28T12:36:54Z2018-08-20T00:00:00ZHandlehttps://hdl.handle.net/1822/61669http://purl.org/coar/access_right/c_abf2open accessMLSAphylogenycalmodulintaxonomyITS regionß-tubulinβ-tubulin2310784 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/62c8c006-827d-4b4e-8255-aee7b42c9e56/download
spellingShingle Penicillium tunisiense sp. nov., a novel species of Penicillium section Ramosa discovered from Tunisian orchard apples
Ouhibi, Salma
MLSA
phylogeny
calmodulin
taxonomy
ITS region
ß-tubulin
β-tubulin
status SINGLETON
subject.fl_str_mv MLSA
phylogeny
calmodulin
taxonomy
ITS region
ß-tubulin
β-tubulin
title Penicillium tunisiense sp. nov., a novel species of Penicillium section Ramosa discovered from Tunisian orchard apples
title_full Penicillium tunisiense sp. nov., a novel species of Penicillium section Ramosa discovered from Tunisian orchard apples
title_fullStr Penicillium tunisiense sp. nov., a novel species of Penicillium section Ramosa discovered from Tunisian orchard apples
title_full_unstemmed Penicillium tunisiense sp. nov., a novel species of Penicillium section Ramosa discovered from Tunisian orchard apples
title_short Penicillium tunisiense sp. nov., a novel species of Penicillium section Ramosa discovered from Tunisian orchard apples
title_sort Penicillium tunisiense sp. nov., a novel species of Penicillium section Ramosa discovered from Tunisian orchard apples
topic MLSA
phylogeny
calmodulin
taxonomy
ITS region
ß-tubulin
β-tubulin
topic_facet MLSA
phylogeny
calmodulin
taxonomy
ITS region
ß-tubulin
β-tubulin
url https://hdl.handle.net/1822/61669
visible 1