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Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells

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Resumo:Aim: To produce marine-origin nanoparticles (NPs) aiming to develop more effective and tolerated therapies for breast cancer. Materials & methods: NPs based in two marine-origin polymers (fucoidan and chitosan) were prepared by polyelectrolyte complexation, for the delivery of an antitumor drug model (gemcitabine [Gem]). Results: Final formulation resulted in stable NPs around 115â 140 nm in size and with a polydispersity index less than 0.2. Gem was encapsulated at a maximum entrapment efficiency of 35â 42%. Drug-release studies demonstrated that around 84% of Gem is released within 4 h. Cytotoxicity results of Gem-loaded NPs showed increased toxicity (around 25%) when compared with free Gem. Conclusion: The drug-loaded NPs present increased toxicity over human breast cancer cells without increasing toxic effects over endothelial cells.
Autores principais:Oliveira, Catarina
Outros Autores:Neves, N. M.; Reis, R. L.; Martins, A.; Silva, T. H.
Assunto:breast cancer cells Chitosan Drug Delivery System endothelial cells Fucoidan gemcitabine Nanoparticles Polyelectrolyte complexation
Ano:2018
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho
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author Oliveira, Catarina
author2 Neves, N. M.
Reis, R. L.
Martins, A.
Silva, T. H.
author2_role author
author
author
author
author_facet Oliveira, Catarina
Neves, N. M.
Reis, R. L.
Martins, A.
Silva, T. H.
author_role author
contributor_name_str_mv Universidade do Minho
country_str PT
creators_json_txt [{\"Person.name\":\"Oliveira, Catarina\"},{\"Person.name\":\"Neves, N. M.\"},{\"Person.name\":\"Reis, R. L.\"},{\"Person.name\":\"Martins, A.\"},{\"Person.name\":\"Silva, T. H.\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Universidade do Minho
datacite.creators.creator.creatorName.fl_str_mv Oliveira, Catarina
Neves, N. M.
Reis, R. L.
Martins, A.
Silva, T. H.
datacite.date.Accepted.fl_str_mv 2018-09-01T00:00:00Z
datacite.date.available.fl_str_mv 2019-01-30T11:05:23Z
datacite.date.embargoed.fl_str_mv 2019-01-30T11:05:23Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv breast cancer cells
Chitosan
Drug Delivery System
endothelial cells
Fucoidan
gemcitabine
Nanoparticles
Polyelectrolyte complexation
datacite.titles.title.fl_str_mv Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells
dc.contributor.none.fl_str_mv Universidade do Minho
dc.creator.none.fl_str_mv Oliveira, Catarina
Neves, N. M.
Reis, R. L.
Martins, A.
Silva, T. H.
dc.date.Accepted.fl_str_mv 2018-09-01T00:00:00Z
dc.date.available.fl_str_mv 2019-01-30T11:05:23Z
dc.date.embargoed.fl_str_mv 2019-01-30T11:05:23Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv https://hdl.handle.net/1822/58734
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv Future Medicine Ltd
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv breast cancer cells
Chitosan
Drug Delivery System
endothelial cells
Fucoidan
gemcitabine
Nanoparticles
Polyelectrolyte complexation
dc.title.fl_str_mv Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description Aim: To produce marine-origin nanoparticles (NPs) aiming to develop more effective and tolerated therapies for breast cancer. Materials & methods: NPs based in two marine-origin polymers (fucoidan and chitosan) were prepared by polyelectrolyte complexation, for the delivery of an antitumor drug model (gemcitabine [Gem]). Results: Final formulation resulted in stable NPs around 115â 140 nm in size and with a polydispersity index less than 0.2. Gem was encapsulated at a maximum entrapment efficiency of 35â 42%. Drug-release studies demonstrated that around 84% of Gem is released within 4 h. Cytotoxicity results of Gem-loaded NPs showed increased toxicity (around 25%) when compared with free Gem. Conclusion: The drug-loaded NPs present increased toxicity over human breast cancer cells without increasing toxic effects over endothelial cells.
dirty 0
eu_rights_str_mv openAccess
format article
fulltext.url.fl_str_mv https://prod-dspace.uminho.pt/bitstreams/c4f8150e-28c4-4800-bbef-b7cb33abb66b/download
id rum_dcbf4df4d0660443f89fe6fb2d24c8bb
identifier.url.fl_str_mv https://hdl.handle.net/1822/58734
instacron_str repositorium
institution Universidade do Minho
instname_str Universidade do Minho
language eng
network_acronym_str rum
network_name_str RepositóriUM - Universidade do Minho
oai_identifier_str oai:repositorium.uminho.pt:1822/58734
organization_str_mv urn:organizationAcronym:repositorium
person_str_mv Oliveira, Catarina
Neves, N. M.
Reis, R. L.
Martins, A.
Silva, T. H.
publishDate 2018
publisher.none.fl_str_mv Future Medicine Ltd
reponame_str RepositóriUM - Universidade do Minho
repository_id_str urn:repositoryAcronym:rum
service_str_mv urn:repositoryAcronym:rum
spelling engFuture Medicine LtdporAim: To produce marine-origin nanoparticles (NPs) aiming to develop more effective and tolerated therapies for breast cancer. Materials & methods: NPs based in two marine-origin polymers (fucoidan and chitosan) were prepared by polyelectrolyte complexation, for the delivery of an antitumor drug model (gemcitabine [Gem]). Results: Final formulation resulted in stable NPs around 115â 140 nm in size and with a polydispersity index less than 0.2. Gem was encapsulated at a maximum entrapment efficiency of 35â 42%. Drug-release studies demonstrated that around 84% of Gem is released within 4 h. Cytotoxicity results of Gem-loaded NPs showed increased toxicity (around 25%) when compared with free Gem. Conclusion: The drug-loaded NPs present increased toxicity over human breast cancer cells without increasing toxic effects over endothelial cells.application/pdfporGemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cellsOliveira, CatarinaNeves, N. M.Reis, R. L.Martins, A.Silva, T. H.HostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf1743-5889DOIIsPartOf10.2217/nnm-2018-00042019-01-30T11:05:23Z2018-092018-112019-01-30T09:50:11Z2018-09-01T00:00:00ZHandlehttps://hdl.handle.net/1822/58734http://purl.org/coar/access_right/c_abf2open accessbreast cancer cellsChitosanDrug Delivery Systemendothelial cellsFucoidangemcitabineNanoparticlesPolyelectrolyte complexation2933782 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/c4f8150e-28c4-4800-bbef-b7cb33abb66b/download
spellingShingle Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells
Oliveira, Catarina
breast cancer cells
Chitosan
Drug Delivery System
endothelial cells
Fucoidan
gemcitabine
Nanoparticles
Polyelectrolyte complexation
status SINGLETON
subject.fl_str_mv breast cancer cells
Chitosan
Drug Delivery System
endothelial cells
Fucoidan
gemcitabine
Nanoparticles
Polyelectrolyte complexation
title Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells
title_full Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells
title_fullStr Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells
title_full_unstemmed Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells
title_short Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells
title_sort Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells
topic breast cancer cells
Chitosan
Drug Delivery System
endothelial cells
Fucoidan
gemcitabine
Nanoparticles
Polyelectrolyte complexation
topic_facet breast cancer cells
Chitosan
Drug Delivery System
endothelial cells
Fucoidan
gemcitabine
Nanoparticles
Polyelectrolyte complexation
url https://hdl.handle.net/1822/58734
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