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Hyperbaric bioreactors use with Yarrowia lipolytica cultures : cellular adaptation to hyperbaric conditions

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Resumo:Increase air pressure for aeration of microbial cell cultures can prevent oxygen limitation but cause oxidative stress and consequently affect cell physiology. A pressurized bioreactor was used for Y. lipolytica batch cultivation under increased air pressure up to 6 bar and no inhibition of cell growth was observed. Moreover, an increase of 6-fold led to a 3.4-fold increase in specific growth rate under atmospheric pressure. The antioxidant enzyme superoxide dismutase was induced by the air pressure raise, which indicates that the defensive mechanisms of the cells were effective and cells could cope with increased pressure. The extracellular lipase activity increased from 22.3 to 43.7 U/l using a 5-bar air pressure instead of air at 1 bar pressure.
Autores principais:Lopes, Marlene
Outros Autores:Gomes, Nelma; Mota, M.; Belo, Isabel
Assunto:Hyperbaric bioreactor Pressure Cell concentration Yarrowia lipolytica Oxidative stress
Ano:2008
País:Portugal
Tipo de documento:comunicação em conferência
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho

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