Publicação
A genomically unique lytic bacteriophage infecting Pseudomonas syringae pv. actinidiae
| Resumo: | Bacterial canker caused by Pseudomonas syringae pv. actinidiae (Psa) continues to be a major threat to kiwifruit production worldwide. Current control methods, focused on chemical treatments and orchard hygiene strategies have low efficacy and pose several limitations, necessitating novel sustainable alternatives. In this study, a new lytic Myovirus bacteriophage, Qdp_Psa3, was isolated and characterized from Psa infected kiwifruit orchard soil. The bacteriophage displayed a narrow host range across Psa biovars (1, 2, and 3), strong pH stability and moderate thermal stability being inhibited at temperatures above 25 °C. Genomic sequencing revealed a 115 kb dsDNA genome lacking antibiotic resistance, tRNA, or virulence genes, and the analysis of the DNA packaging cassette suggested an uncharacterized mechanism. Phylogenetic and comparative genomic analyses support its designation as a novel species within the Caudoviricetes class, with proteomic profiling identified 62 virion-associated proteins, 71% of which remains hypothetical. Qdp_Psa3 demonstrated potential as a biocontrol agent, representing a promising candidate for inclusion as a complementary bacteriophage in a phage cocktail. Its discovery provides valuable insights into the hidden viral diversity of kiwifruit soils and expands our understanding of bacteriophagePsa interactions. |
|---|---|
| Autores principais: | Gimranov, Emil |
| Outros Autores: | Oliveira, Hugo Alexandre Mendes; Santos, Sílvio Roberto Branco; Weber, Moritz; Höfer, Katharina; Santos, Conceição; Moura, Luísa; Azeredo, Joana |
| Assunto: | Pseudomonas syringae pv. actinidiae Myovirus Bacterial canker Biological control Bacteriophage |
| Ano: | 2026 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867437958945046528 |
|---|---|
| author | Gimranov, Emil |
| author2 | Oliveira, Hugo Alexandre Mendes Santos, Sílvio Roberto Branco Weber, Moritz Höfer, Katharina Santos, Conceição Moura, Luísa Azeredo, Joana |
| author2_role | author author author author author author author |
| author_facet | Gimranov, Emil Oliveira, Hugo Alexandre Mendes Santos, Sílvio Roberto Branco Weber, Moritz Höfer, Katharina Santos, Conceição Moura, Luísa Azeredo, Joana |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Gimranov, Emil\"},{\"Person.name\":\"Oliveira, Hugo Alexandre Mendes\"},{\"Person.name\":\"Santos, Sílvio Roberto Branco\"},{\"Person.name\":\"Weber, Moritz\"},{\"Person.name\":\"Höfer, Katharina\"},{\"Person.name\":\"Santos, Conceição\"},{\"Person.name\":\"Moura, Luísa\"},{\"Person.name\":\"Azeredo, Joana\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Gimranov, Emil Oliveira, Hugo Alexandre Mendes Santos, Sílvio Roberto Branco Weber, Moritz Höfer, Katharina Santos, Conceição Moura, Luísa Azeredo, Joana |
| datacite.date.Accepted.fl_str_mv | 2026-01-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Pseudomonas syringae pv. actinidiae Myovirus Bacterial canker Biological control Bacteriophage |
| datacite.titles.title.fl_str_mv | A genomically unique lytic bacteriophage infecting Pseudomonas syringae pv. actinidiae |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Gimranov, Emil Oliveira, Hugo Alexandre Mendes Santos, Sílvio Roberto Branco Weber, Moritz Höfer, Katharina Santos, Conceição Moura, Luísa Azeredo, Joana |
| dc.date.Accepted.fl_str_mv | 2026-01-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/100752 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.rights.rights.copyright.fl_str_mv | openAccess |
| dc.subject.none.fl_str_mv | Pseudomonas syringae pv. actinidiae Myovirus Bacterial canker Biological control Bacteriophage |
| dc.title.fl_str_mv | A genomically unique lytic bacteriophage infecting Pseudomonas syringae pv. actinidiae |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Bacterial canker caused by Pseudomonas syringae pv. actinidiae (Psa) continues to be a major threat to kiwifruit production worldwide. Current control methods, focused on chemical treatments and orchard hygiene strategies have low efficacy and pose several limitations, necessitating novel sustainable alternatives. In this study, a new lytic Myovirus bacteriophage, Qdp_Psa3, was isolated and characterized from Psa infected kiwifruit orchard soil. The bacteriophage displayed a narrow host range across Psa biovars (1, 2, and 3), strong pH stability and moderate thermal stability being inhibited at temperatures above 25 °C. Genomic sequencing revealed a 115 kb dsDNA genome lacking antibiotic resistance, tRNA, or virulence genes, and the analysis of the DNA packaging cassette suggested an uncharacterized mechanism. Phylogenetic and comparative genomic analyses support its designation as a novel species within the Caudoviricetes class, with proteomic profiling identified 62 virion-associated proteins, 71% of which remains hypothetical. Qdp_Psa3 demonstrated potential as a biocontrol agent, representing a promising candidate for inclusion as a complementary bacteriophage in a phage cocktail. Its discovery provides valuable insights into the hidden viral diversity of kiwifruit soils and expands our understanding of bacteriophagePsa interactions. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/ea435f98-040d-42e8-8298-c83af69e5116/download |
| id | rum_e29d7684e7f5bb4604521e8c5bc8defd |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/100752 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/100752 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Gimranov, Emil Oliveira, Hugo Alexandre Mendes Santos, Sílvio Roberto Branco Weber, Moritz Höfer, Katharina Santos, Conceição Moura, Luísa Azeredo, Joana |
| publishDate | 2026 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engElsevierengBacterial canker caused by Pseudomonas syringae pv. actinidiae (Psa) continues to be a major threat to kiwifruit production worldwide. Current control methods, focused on chemical treatments and orchard hygiene strategies have low efficacy and pose several limitations, necessitating novel sustainable alternatives. In this study, a new lytic Myovirus bacteriophage, Qdp_Psa3, was isolated and characterized from Psa infected kiwifruit orchard soil. The bacteriophage displayed a narrow host range across Psa biovars (1, 2, and 3), strong pH stability and moderate thermal stability being inhibited at temperatures above 25 °C. Genomic sequencing revealed a 115 kb dsDNA genome lacking antibiotic resistance, tRNA, or virulence genes, and the analysis of the DNA packaging cassette suggested an uncharacterized mechanism. Phylogenetic and comparative genomic analyses support its designation as a novel species within the Caudoviricetes class, with proteomic profiling identified 62 virion-associated proteins, 71% of which remains hypothetical. Qdp_Psa3 demonstrated potential as a biocontrol agent, representing a promising candidate for inclusion as a complementary bacteriophage in a phage cocktail. Its discovery provides valuable insights into the hidden viral diversity of kiwifruit soils and expands our understanding of bacteriophagePsa interactions.application/pdfengA genomically unique lytic bacteriophage infecting Pseudomonas syringae pv. actinidiaeGimranov, EmilOliveira, Hugo Alexandre MendesSantos, Sílvio Roberto BrancoWeber, MoritzHöfer, KatharinaSantos, ConceiçãoMoura, LuísaAzeredo, JoanaHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONGimranov, Emil; Oliveira, Hugo; Santos, Sílvio Roberto Branco; Weber, Moritz; Höfer, Katharina; Santos, Conceição; Moura, Luísa; Azeredo, Joana, A genomically unique lytic bacteriophage infecting Pseudomonas syringae pv. actinidiae. Virology, 619(110864), 2026ARTICLENUMBER110864ISSNIsPartOf0042-6822EISSNIsPartOf1096-0341DOIIsPartOf10.1016/j.virol.2026.11086420262026-03-21T18:46:02Z2026-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/100752http://purl.org/coar/access_right/c_abf2open accessPseudomonas syringae pv. actinidiaeMyovirusBacterial cankerBiological controlBacteriophage6540468 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2026http://creativecommons.org/licenses/by/4.0/openAccesshttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/ea435f98-040d-42e8-8298-c83af69e5116/download619 |
| spellingShingle | A genomically unique lytic bacteriophage infecting Pseudomonas syringae pv. actinidiae Gimranov, Emil Pseudomonas syringae pv. actinidiae Myovirus Bacterial canker Biological control Bacteriophage |
| status | SINGLETON |
| subject.fl_str_mv | Pseudomonas syringae pv. actinidiae Myovirus Bacterial canker Biological control Bacteriophage |
| title | A genomically unique lytic bacteriophage infecting Pseudomonas syringae pv. actinidiae |
| title_full | A genomically unique lytic bacteriophage infecting Pseudomonas syringae pv. actinidiae |
| title_fullStr | A genomically unique lytic bacteriophage infecting Pseudomonas syringae pv. actinidiae |
| title_full_unstemmed | A genomically unique lytic bacteriophage infecting Pseudomonas syringae pv. actinidiae |
| title_short | A genomically unique lytic bacteriophage infecting Pseudomonas syringae pv. actinidiae |
| title_sort | A genomically unique lytic bacteriophage infecting Pseudomonas syringae pv. actinidiae |
| topic | Pseudomonas syringae pv. actinidiae Myovirus Bacterial canker Biological control Bacteriophage |
| topic_facet | Pseudomonas syringae pv. actinidiae Myovirus Bacterial canker Biological control Bacteriophage |
| url | https://hdl.handle.net/1822/100752 |
| visible | 1 |