Publicação
Liquid-liquid equilibrium and partitioning features of bovine trypsin in Ucon 50 HB5100 /sodium citrate aqueous two phase systems
| Resumo: | The phase diagrams of Ucon 50-HB-5100, a non-ionic random copolymer of ethylene oxide and propylene oxide (EOPO) and sodium citrate aqueous two-phase systems were determined at different pHs (5.20 and 8.20) and temperatures (5, 20 and 40º C). The binodal curves were determined by refractive index and enzymatic assay of the solution and described using a four-parameter sigmoidal equation, the reliability of the measured tie line compositions was ascertained by correlation equations given by Othmer Tobias and Bancroft. The two-phase area was expanded by increasing both pH and temperature. The partitioning of bovine trypsin and a-chymotrypsin, proteases of similar physico-chemical properties was investigated in order to evaluate the applicability of partitioning as a putative method to isolate from pancreas and to obtain any information about their partitioning mechanism. The effect of different factors such as pH, tie line length and the presence of an inorganic salt on the protein partition coefficient were analyzed. |
|---|---|
| Autores principais: | Tubío, Gisela |
| Outros Autores: | Venâncio, Armando; Teixeira, J. A.; Nerli, Bibiana B.; Picó, Guillermo A. |
| Ano: | 2008 |
| País: | Portugal |
| Tipo de documento: | capítulo de livro |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439120029057024 |
|---|---|
| author | Tubío, Gisela |
| author2 | Venâncio, Armando Teixeira, J. A. Nerli, Bibiana B. Picó, Guillermo A. |
| author2_role | author author author author |
| author_facet | Tubío, Gisela Venâncio, Armando Teixeira, J. A. Nerli, Bibiana B. Picó, Guillermo A. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Tubío, Gisela\"},{\"Person.name\":\"Venâncio, Armando\"},{\"Person.name\":\"Teixeira, J. A.\"},{\"Person.name\":\"Nerli, Bibiana B.\"},{\"Person.name\":\"Picó, Guillermo A.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Tubío, Gisela Venâncio, Armando Teixeira, J. A. Nerli, Bibiana B. Picó, Guillermo A. |
| datacite.date.Accepted.fl_str_mv | 2008-09-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2015-02-13T11:26:51Z |
| datacite.date.embargoed.fl_str_mv | 2015-02-13T11:26:51Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.titles.title.fl_str_mv | Liquid-liquid equilibrium and partitioning features of bovine trypsin in Ucon 50 HB5100 /sodium citrate aqueous two phase systems |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Tubío, Gisela Venâncio, Armando Teixeira, J. A. Nerli, Bibiana B. Picó, Guillermo A. |
| dc.date.Accepted.fl_str_mv | 2008-09-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2015-02-13T11:26:51Z |
| dc.date.embargoed.fl_str_mv | 2015-02-13T11:26:51Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/33853 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.title.fl_str_mv | Liquid-liquid equilibrium and partitioning features of bovine trypsin in Ucon 50 HB5100 /sodium citrate aqueous two phase systems |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_3248 |
| description | The phase diagrams of Ucon 50-HB-5100, a non-ionic random copolymer of ethylene oxide and propylene oxide (EOPO) and sodium citrate aqueous two-phase systems were determined at different pHs (5.20 and 8.20) and temperatures (5, 20 and 40º C). The binodal curves were determined by refractive index and enzymatic assay of the solution and described using a four-parameter sigmoidal equation, the reliability of the measured tie line compositions was ascertained by correlation equations given by Othmer Tobias and Bancroft. The two-phase area was expanded by increasing both pH and temperature. The partitioning of bovine trypsin and a-chymotrypsin, proteases of similar physico-chemical properties was investigated in order to evaluate the applicability of partitioning as a putative method to isolate from pancreas and to obtain any information about their partitioning mechanism. The effect of different factors such as pH, tie line length and the presence of an inorganic salt on the protein partition coefficient were analyzed. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | bookPart |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/300f0d1d-7eb0-4f0a-8c5a-557fc2ca2a40/download |
| id | rum_e3c1ffca4e4e8bad5bc590ef55d97098 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/33853 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/33853 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Tubío, Gisela Venâncio, Armando Teixeira, J. A. Nerli, Bibiana B. Picó, Guillermo A. |
| publishDate | 2008 |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engporThe phase diagrams of Ucon 50-HB-5100, a non-ionic random copolymer of ethylene oxide and propylene oxide (EOPO) and sodium citrate aqueous two-phase systems were determined at different pHs (5.20 and 8.20) and temperatures (5, 20 and 40º C). The binodal curves were determined by refractive index and enzymatic assay of the solution and described using a four-parameter sigmoidal equation, the reliability of the measured tie line compositions was ascertained by correlation equations given by Othmer Tobias and Bancroft. The two-phase area was expanded by increasing both pH and temperature. The partitioning of bovine trypsin and a-chymotrypsin, proteases of similar physico-chemical properties was investigated in order to evaluate the applicability of partitioning as a putative method to isolate from pancreas and to obtain any information about their partitioning mechanism. The effect of different factors such as pH, tie line length and the presence of an inorganic salt on the protein partition coefficient were analyzed.application/pdfporLiquid-liquid equilibrium and partitioning features of bovine trypsin in Ucon 50 HB5100 /sodium citrate aqueous two phase systemsTubío, GiselaVenâncio, ArmandoTeixeira, J. A.Nerli, Bibiana B.Picó, Guillermo A.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONTubío, Gisela; Venâncio, Armando; Teixeira, J. A.; Nerli, B. B.; Picó, G. A., Liquid-liquid equilibrium and partitioning features of bovine trypsin in Ucon 50 HB5100 /sodium citrate aqueous two phase systems. VALNATURA - A Europe-Latin América post-graduate research network in the VALorization of NATURAL resources, 2008. ISBN: 978-972-97810-5-6, 151-158ISBNIsPartOf978-972-97810-5-62015-02-13T11:26:51Z2008-092015-02-09T18:05:55Z2008-09-01T00:00:00ZHandlehttps://hdl.handle.net/1822/33853http://purl.org/coar/access_right/c_abf2open access291556 bytesliteraturehttp://purl.org/coar/resource_type/c_3248book parthttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/300f0d1d-7eb0-4f0a-8c5a-557fc2ca2a40/download |
| spellingShingle | Liquid-liquid equilibrium and partitioning features of bovine trypsin in Ucon 50 HB5100 /sodium citrate aqueous two phase systems Tubío, Gisela |
| status | SINGLETON |
| title | Liquid-liquid equilibrium and partitioning features of bovine trypsin in Ucon 50 HB5100 /sodium citrate aqueous two phase systems |
| title_full | Liquid-liquid equilibrium and partitioning features of bovine trypsin in Ucon 50 HB5100 /sodium citrate aqueous two phase systems |
| title_fullStr | Liquid-liquid equilibrium and partitioning features of bovine trypsin in Ucon 50 HB5100 /sodium citrate aqueous two phase systems |
| title_full_unstemmed | Liquid-liquid equilibrium and partitioning features of bovine trypsin in Ucon 50 HB5100 /sodium citrate aqueous two phase systems |
| title_short | Liquid-liquid equilibrium and partitioning features of bovine trypsin in Ucon 50 HB5100 /sodium citrate aqueous two phase systems |
| title_sort | Liquid-liquid equilibrium and partitioning features of bovine trypsin in Ucon 50 HB5100 /sodium citrate aqueous two phase systems |
| url | https://hdl.handle.net/1822/33853 |
| visible | 1 |