Publicação
Characterization of biofilm formation on a humic material
| Resumo: | Biofilms are major sites of carbon cycling in streams. Therefore, it is crucial to improve knowledge about biofilms’ structure and microbial composition to understand their contribution in the self-purification of surface water. The present work intends to study biofilm formation in the presence of humic substances (HSs) as a carbon source. Two biofilm flowcells were operated in parallel; one with synthetic stream water, displaying a background carbon concentration of 1.26 ± 0.84 mg L−1, the other with added HSs and an overall carbon concentration of 9.68 ± 1.00 mg L−1. From the biofilms’ results of culturable and total countable cells, it can be concluded that the presence of HSs did not significantly enhance the biofilm cell density. However, the biofilm formed in the presence of HSs presented slightly higher values of volatile suspended solids (VSS) and protein. One possible explanation for this result is that HSs adsorbed to the polymeric matrix of the biofilm and were included in the quantification of VSS and protein. The microbial composition of the biofilm with addition of HSs was characterized by the presence of bacteria belonging to beta-Proteobacteria, Cupriavidus metallidurans and several species of the genus Ralstonia were identified, and gamma-Proteobacteria, represented by Escherichia coli. In the biofilm formed without HSs addition beta-Proteobacteria, represented by the species Variovorax paradoxus, and bacteria belonging to the group Bacteroidetes were detected. In conclusion, the presence of HSs did not significantly enhance biofilm cell density but influenced the bacterial diversity in the biofilm. |
|---|---|
| Autores principais: | Rodrigues, A. L. |
| Outros Autores: | Brito, A. G.; Janknecht, P.; Silva, J.; Machado, A. V.; Nogueira, R. |
| Assunto: | Humic material Biofilm Flowcell Community composition FTIR |
| Ano: | 2008 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866876349348577280 |
|---|---|
| author | Rodrigues, A. L. |
| author2 | Brito, A. G. Janknecht, P. Silva, J. Machado, A. V. Nogueira, R. |
| author2_role | author author author author author |
| author_facet | Rodrigues, A. L. Brito, A. G. Janknecht, P. Silva, J. Machado, A. V. Nogueira, R. |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Rodrigues, A. L.\"},{\"Person.name\":\"Brito, A. G.\"},{\"Person.name\":\"Janknecht, P.\"},{\"Person.name\":\"Silva, J.\"},{\"Person.name\":\"Machado, A. V.\"},{\"Person.name\":\"Nogueira, R.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Rodrigues, A. L. Brito, A. G. Janknecht, P. Silva, J. Machado, A. V. Nogueira, R. |
| datacite.date.Accepted.fl_str_mv | 2008-11-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2008-11-24T17:31:26Z |
| datacite.date.embargoed.fl_str_mv | 2008-11-24T17:31:26Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Humic material Biofilm Flowcell Community composition FTIR |
| datacite.titles.title.fl_str_mv | Characterization of biofilm formation on a humic material |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Rodrigues, A. L. Brito, A. G. Janknecht, P. Silva, J. Machado, A. V. Nogueira, R. |
| dc.date.Accepted.fl_str_mv | 2008-11-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2008-11-24T17:31:26Z |
| dc.date.embargoed.fl_str_mv | 2008-11-24T17:31:26Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/8355 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Springer |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Humic material Biofilm Flowcell Community composition FTIR |
| dc.title.fl_str_mv | Characterization of biofilm formation on a humic material |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Biofilms are major sites of carbon cycling in streams. Therefore, it is crucial to improve knowledge about biofilms’ structure and microbial composition to understand their contribution in the self-purification of surface water. The present work intends to study biofilm formation in the presence of humic substances (HSs) as a carbon source. Two biofilm flowcells were operated in parallel; one with synthetic stream water, displaying a background carbon concentration of 1.26 ± 0.84 mg L−1, the other with added HSs and an overall carbon concentration of 9.68 ± 1.00 mg L−1. From the biofilms’ results of culturable and total countable cells, it can be concluded that the presence of HSs did not significantly enhance the biofilm cell density. However, the biofilm formed in the presence of HSs presented slightly higher values of volatile suspended solids (VSS) and protein. One possible explanation for this result is that HSs adsorbed to the polymeric matrix of the biofilm and were included in the quantification of VSS and protein. The microbial composition of the biofilm with addition of HSs was characterized by the presence of bacteria belonging to beta-Proteobacteria, Cupriavidus metallidurans and several species of the genus Ralstonia were identified, and gamma-Proteobacteria, represented by Escherichia coli. In the biofilm formed without HSs addition beta-Proteobacteria, represented by the species Variovorax paradoxus, and bacteria belonging to the group Bacteroidetes were detected. In conclusion, the presence of HSs did not significantly enhance biofilm cell density but influenced the bacterial diversity in the biofilm. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/6ee015b6-3163-4302-9d29-f870114873eb/download |
| id | rum_ea7be49babf2fa4fd8ca1767805cba4c |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/8355 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/8355 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Rodrigues, A. L. Brito, A. G. Janknecht, P. Silva, J. Machado, A. V. Nogueira, R. |
| publishDate | 2008 |
| publisher.none.fl_str_mv | Springer |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engSpringerenBiofilms are major sites of carbon cycling in streams. Therefore, it is crucial to improve knowledge about biofilms’ structure and microbial composition to understand their contribution in the self-purification of surface water. The present work intends to study biofilm formation in the presence of humic substances (HSs) as a carbon source. Two biofilm flowcells were operated in parallel; one with synthetic stream water, displaying a background carbon concentration of 1.26 ± 0.84 mg L−1, the other with added HSs and an overall carbon concentration of 9.68 ± 1.00 mg L−1. From the biofilms’ results of culturable and total countable cells, it can be concluded that the presence of HSs did not significantly enhance the biofilm cell density. However, the biofilm formed in the presence of HSs presented slightly higher values of volatile suspended solids (VSS) and protein. One possible explanation for this result is that HSs adsorbed to the polymeric matrix of the biofilm and were included in the quantification of VSS and protein. The microbial composition of the biofilm with addition of HSs was characterized by the presence of bacteria belonging to beta-Proteobacteria, Cupriavidus metallidurans and several species of the genus Ralstonia were identified, and gamma-Proteobacteria, represented by Escherichia coli. In the biofilm formed without HSs addition beta-Proteobacteria, represented by the species Variovorax paradoxus, and bacteria belonging to the group Bacteroidetes were detected. In conclusion, the presence of HSs did not significantly enhance biofilm cell density but influenced the bacterial diversity in the biofilm.application/pdfenCharacterization of biofilm formation on a humic materialRodrigues, A. L.Brito, A. G.Janknecht, P.Silva, J.Machado, A. V.Nogueira, R.HostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf1367-5435ISSNIsPartOf1476-5535DOIIsPartOf10.1007/s10295-008-0424-82008-11-24T17:31:26Z2008-112008-11-01T00:00:00ZHandlehttps://hdl.handle.net/1822/8355http://purl.org/coar/access_right/c_abf2open accessHumic materialBiofilmFlowcellCommunity compositionFTIR398803 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/6ee015b6-3163-4302-9d29-f870114873eb/download |
| spellingShingle | Characterization of biofilm formation on a humic material Rodrigues, A. L. Humic material Biofilm Flowcell Community composition FTIR |
| status | SINGLETON |
| subject.fl_str_mv | Humic material Biofilm Flowcell Community composition FTIR |
| title | Characterization of biofilm formation on a humic material |
| title_full | Characterization of biofilm formation on a humic material |
| title_fullStr | Characterization of biofilm formation on a humic material |
| title_full_unstemmed | Characterization of biofilm formation on a humic material |
| title_short | Characterization of biofilm formation on a humic material |
| title_sort | Characterization of biofilm formation on a humic material |
| topic | Humic material Biofilm Flowcell Community composition FTIR |
| topic_facet | Humic material Biofilm Flowcell Community composition FTIR |
| url | https://hdl.handle.net/1822/8355 |
| visible | 1 |