Publicação
Biofilms of non-Candida albicans Candida species : quantification, structure and matrix composition
| Resumo: | Most cases of candidiasis have been attributed to C. albicans, but recently, non- Candida albicans Candida (NCAC) species have been identified as common pathogens. The ability of Candida species to form biofilms has important clinical repercussions due to their increased resistance to antifungal therapy and the ability of yeast cells within the biofilms to withstand host immune defenses. Given this clinical importance of the biofilm growth form, the aim of this study was to characterize biofilms produced by three NCAC species, namely C. parapsilosis, C. tropicalis and C. glabrata. The biofilm forming ability of clinical isolates of C. parapsilosis, C. tropicalis and C. glabrata recovered from different sources, was evaluated by crystal violet staining. The structure and morphological characteristics of the biofilms were also assessed by scanning electron microscopy and the biofilm matrix composition analyzed for protein and carbohydrate content. All NCAC species were able to form biofilms although these were less extensive for C. glabrata compared with C. parapsilosis and C. tropicalis. It was evident that C. parapsilosis biofilm production was highly strain dependent, a feature not evident with C. glabrata and C. tropicalis. Scanning electron microscopy revealed structural differences for biofilms with respect to cell morphology and spatial arrangement. Candida parapsilosis biofilm matrices had large amounts of carbohydrate with less protein. Conversely, matrices extracted from C. tropicalis biofilms had low amounts of carbohydrate and protein. Interestingly, C. glabrata biofilm matrix was high in both protein and carbohydrate content. The present work demonstrates that biofilm forming ability, structure and matrix composition are highly species dependent with additional strain variability occurring with C. parapsilosis. |
|---|---|
| Autores principais: | Silva, Sónia Carina |
| Outros Autores: | Henriques, Mariana; Martins, António; Oliveira, Rosário; Williams, David; Azeredo, Joana |
| Assunto: | Biofilm non-Candida albicans Candida species |
| Ano: | 2009 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866878078607687680 |
|---|---|
| author | Silva, Sónia Carina |
| author2 | Henriques, Mariana Martins, António Oliveira, Rosário Williams, David Azeredo, Joana |
| author2_role | author author author author author |
| author_facet | Silva, Sónia Carina Henriques, Mariana Martins, António Oliveira, Rosário Williams, David Azeredo, Joana |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Silva, Sónia Carina\"},{\"Person.name\":\"Henriques, Mariana\"},{\"Person.name\":\"Martins, António\"},{\"Person.name\":\"Oliveira, Rosário\"},{\"Person.name\":\"Williams, David\"},{\"Person.name\":\"Azeredo, Joana\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Silva, Sónia Carina Henriques, Mariana Martins, António Oliveira, Rosário Williams, David Azeredo, Joana |
| datacite.date.Accepted.fl_str_mv | 2009-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2010-04-12T16:21:57Z |
| datacite.date.embargoed.fl_str_mv | 2010-04-12T16:21:57Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Biofilm non-Candida albicans Candida species |
| datacite.titles.title.fl_str_mv | Biofilms of non-Candida albicans Candida species : quantification, structure and matrix composition |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Silva, Sónia Carina Henriques, Mariana Martins, António Oliveira, Rosário Williams, David Azeredo, Joana |
| dc.date.Accepted.fl_str_mv | 2009-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2010-04-12T16:21:57Z |
| dc.date.embargoed.fl_str_mv | 2010-04-12T16:21:57Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/10514 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Informa Healthcare |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Biofilm non-Candida albicans Candida species |
| dc.title.fl_str_mv | Biofilms of non-Candida albicans Candida species : quantification, structure and matrix composition |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Most cases of candidiasis have been attributed to C. albicans, but recently, non- Candida albicans Candida (NCAC) species have been identified as common pathogens. The ability of Candida species to form biofilms has important clinical repercussions due to their increased resistance to antifungal therapy and the ability of yeast cells within the biofilms to withstand host immune defenses. Given this clinical importance of the biofilm growth form, the aim of this study was to characterize biofilms produced by three NCAC species, namely C. parapsilosis, C. tropicalis and C. glabrata. The biofilm forming ability of clinical isolates of C. parapsilosis, C. tropicalis and C. glabrata recovered from different sources, was evaluated by crystal violet staining. The structure and morphological characteristics of the biofilms were also assessed by scanning electron microscopy and the biofilm matrix composition analyzed for protein and carbohydrate content. All NCAC species were able to form biofilms although these were less extensive for C. glabrata compared with C. parapsilosis and C. tropicalis. It was evident that C. parapsilosis biofilm production was highly strain dependent, a feature not evident with C. glabrata and C. tropicalis. Scanning electron microscopy revealed structural differences for biofilms with respect to cell morphology and spatial arrangement. Candida parapsilosis biofilm matrices had large amounts of carbohydrate with less protein. Conversely, matrices extracted from C. tropicalis biofilms had low amounts of carbohydrate and protein. Interestingly, C. glabrata biofilm matrix was high in both protein and carbohydrate content. The present work demonstrates that biofilm forming ability, structure and matrix composition are highly species dependent with additional strain variability occurring with C. parapsilosis. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/3534e89e-7191-450e-b2cf-d5264fc1eb19/download |
| id | rum_eba2d5edfef14e9ec7a2a40e5bfa6f02 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/10514 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/10514 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Silva, Sónia Carina Henriques, Mariana Martins, António Oliveira, Rosário Williams, David Azeredo, Joana |
| publishDate | 2009 |
| publisher.none.fl_str_mv | Informa Healthcare |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engInforma HealthcareporMost cases of candidiasis have been attributed to C. albicans, but recently, non- Candida albicans Candida (NCAC) species have been identified as common pathogens. The ability of Candida species to form biofilms has important clinical repercussions due to their increased resistance to antifungal therapy and the ability of yeast cells within the biofilms to withstand host immune defenses. Given this clinical importance of the biofilm growth form, the aim of this study was to characterize biofilms produced by three NCAC species, namely C. parapsilosis, C. tropicalis and C. glabrata. The biofilm forming ability of clinical isolates of C. parapsilosis, C. tropicalis and C. glabrata recovered from different sources, was evaluated by crystal violet staining. The structure and morphological characteristics of the biofilms were also assessed by scanning electron microscopy and the biofilm matrix composition analyzed for protein and carbohydrate content. All NCAC species were able to form biofilms although these were less extensive for C. glabrata compared with C. parapsilosis and C. tropicalis. It was evident that C. parapsilosis biofilm production was highly strain dependent, a feature not evident with C. glabrata and C. tropicalis. Scanning electron microscopy revealed structural differences for biofilms with respect to cell morphology and spatial arrangement. Candida parapsilosis biofilm matrices had large amounts of carbohydrate with less protein. Conversely, matrices extracted from C. tropicalis biofilms had low amounts of carbohydrate and protein. Interestingly, C. glabrata biofilm matrix was high in both protein and carbohydrate content. The present work demonstrates that biofilm forming ability, structure and matrix composition are highly species dependent with additional strain variability occurring with C. parapsilosis.application/pdfporBiofilms of non-Candida albicans Candida species : quantification, structure and matrix compositionSilva, Sónia CarinaHenriques, MarianaMartins, AntónioOliveira, RosárioWilliams, DavidAzeredo, JoanaHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf1369-3786ISSNIsPartOf1460-2709DOIIsPartOf10.3109/136937808025495942010-04-12T16:21:57Z20092009-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/10514http://purl.org/coar/access_right/c_abf2open accessBiofilmnon-Candida albicans Candida species1422617 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/3534e89e-7191-450e-b2cf-d5264fc1eb19/download |
| spellingShingle | Biofilms of non-Candida albicans Candida species : quantification, structure and matrix composition Silva, Sónia Carina Biofilm non-Candida albicans Candida species |
| status | SINGLETON |
| subject.fl_str_mv | Biofilm non-Candida albicans Candida species |
| title | Biofilms of non-Candida albicans Candida species : quantification, structure and matrix composition |
| title_full | Biofilms of non-Candida albicans Candida species : quantification, structure and matrix composition |
| title_fullStr | Biofilms of non-Candida albicans Candida species : quantification, structure and matrix composition |
| title_full_unstemmed | Biofilms of non-Candida albicans Candida species : quantification, structure and matrix composition |
| title_short | Biofilms of non-Candida albicans Candida species : quantification, structure and matrix composition |
| title_sort | Biofilms of non-Candida albicans Candida species : quantification, structure and matrix composition |
| topic | Biofilm non-Candida albicans Candida species |
| topic_facet | Biofilm non-Candida albicans Candida species |
| url | https://hdl.handle.net/1822/10514 |
| visible | 1 |