Publicação
Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties
| Resumo: | This work studied the physical immobilization of a commercial laccase on bacterial nanocellulose (BNC) aiming to identify the laccase antibacterial properties suitable for wound dressings. Physico-chemical analysis demonstrates that the BNC structure is manly formed by pure crystalline I cellulose. The pH optimum and activation energy of free laccase depends on the substrate employed corresponding to pH 6, 7, 3 and 57, 22, 48 kJ mol1 for 2,6-dimethylphenol (DMP), catechol and 2,2 -azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), respectively. The Michaelis-Menten constant (Km) value for the immobilized laccase (0.77 mM) was found to be almost double of that of the free enzyme (0.42 mM). However, the specific activities of immobilized and free laccase are similar suggesting that the cage-like structure of BNC allows entrapped laccase to maintain some flexibility and favour substrate accessibility. The results clearly show the antimicrobial effect of laccase in Gram-positive (92%) and Gram-negative (26%) bacteria and cytotoxicity acceptable for wound dressing applications. |
|---|---|
| Autores principais: | Sampaio, Liliana M. P. |
| Outros Autores: | Padrão, Jorge; Faria, Jorge; Silva, João P.; Silva, Carla J.; Dourado, Fernando; Zille, Andrea |
| Assunto: | Bacterial Cellulose Laccase Antimicrobial Wound Dressing Immobilization Cytotoxicity |
| Ano: | 2016 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866876243061768192 |
|---|---|
| author | Sampaio, Liliana M. P. |
| author2 | Padrão, Jorge Faria, Jorge Silva, João P. Silva, Carla J. Dourado, Fernando Zille, Andrea |
| author2_role | author author author author author author |
| author_facet | Sampaio, Liliana M. P. Padrão, Jorge Faria, Jorge Silva, João P. Silva, Carla J. Dourado, Fernando Zille, Andrea |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Sampaio, Liliana M. P.\"},{\"Person.name\":\"Padrão, Jorge\"},{\"Person.name\":\"Faria, Jorge\"},{\"Person.name\":\"Silva, João P.\"},{\"Person.name\":\"Silva, Carla J.\"},{\"Person.name\":\"Dourado, Fernando\"},{\"Person.name\":\"Zille, Andrea\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Sampaio, Liliana M. P. Padrão, Jorge Faria, Jorge Silva, João P. Silva, Carla J. Dourado, Fernando Zille, Andrea |
| datacite.date.Accepted.fl_str_mv | 2016-07-10T00:00:00Z |
| datacite.date.available.fl_str_mv | 2016-04-27T16:38:13Z |
| datacite.date.embargoed.fl_str_mv | 2016-04-27T16:38:13Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Bacterial Cellulose Laccase Antimicrobial Wound Dressing Immobilization Cytotoxicity |
| datacite.titles.title.fl_str_mv | Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Sampaio, Liliana M. P. Padrão, Jorge Faria, Jorge Silva, João P. Silva, Carla J. Dourado, Fernando Zille, Andrea |
| dc.date.Accepted.fl_str_mv | 2016-07-10T00:00:00Z |
| dc.date.available.fl_str_mv | 2016-04-27T16:38:13Z |
| dc.date.embargoed.fl_str_mv | 2016-04-27T16:38:13Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/41296 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Bacterial Cellulose Laccase Antimicrobial Wound Dressing Immobilization Cytotoxicity |
| dc.title.fl_str_mv | Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | This work studied the physical immobilization of a commercial laccase on bacterial nanocellulose (BNC) aiming to identify the laccase antibacterial properties suitable for wound dressings. Physico-chemical analysis demonstrates that the BNC structure is manly formed by pure crystalline I cellulose. The pH optimum and activation energy of free laccase depends on the substrate employed corresponding to pH 6, 7, 3 and 57, 22, 48 kJ mol1 for 2,6-dimethylphenol (DMP), catechol and 2,2 -azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), respectively. The Michaelis-Menten constant (Km) value for the immobilized laccase (0.77 mM) was found to be almost double of that of the free enzyme (0.42 mM). However, the specific activities of immobilized and free laccase are similar suggesting that the cage-like structure of BNC allows entrapped laccase to maintain some flexibility and favour substrate accessibility. The results clearly show the antimicrobial effect of laccase in Gram-positive (92%) and Gram-negative (26%) bacteria and cytotoxicity acceptable for wound dressing applications. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/7d8c4543-fbf4-4cb9-abbd-b79d0195e7f7/download |
| id | rum_ee3dcb00f32ea5a2ea5441ad8558fb16 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/41296 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/41296 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Sampaio, Liliana M. P. Padrão, Jorge Faria, Jorge Silva, João P. Silva, Carla J. Dourado, Fernando Zille, Andrea |
| publishDate | 2016 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engElsevierporThis work studied the physical immobilization of a commercial laccase on bacterial nanocellulose (BNC) aiming to identify the laccase antibacterial properties suitable for wound dressings. Physico-chemical analysis demonstrates that the BNC structure is manly formed by pure crystalline I cellulose. The pH optimum and activation energy of free laccase depends on the substrate employed corresponding to pH 6, 7, 3 and 57, 22, 48 kJ mol1 for 2,6-dimethylphenol (DMP), catechol and 2,2 -azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), respectively. The Michaelis-Menten constant (Km) value for the immobilized laccase (0.77 mM) was found to be almost double of that of the free enzyme (0.42 mM). However, the specific activities of immobilized and free laccase are similar suggesting that the cage-like structure of BNC allows entrapped laccase to maintain some flexibility and favour substrate accessibility. The results clearly show the antimicrobial effect of laccase in Gram-positive (92%) and Gram-negative (26%) bacteria and cytotoxicity acceptable for wound dressing applications.application/pdfporLaccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability propertiesSampaio, Liliana M. P.Padrão, JorgeFaria, JorgeSilva, João P.Silva, Carla J.Dourado, FernandoZille, AndreaHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf0144-8617DOIIsPartOf10.1016/j.carbpol.2016.03.0092016-04-27T16:38:13Z2016-07-102016-04-27T13:41:13Z2016-07-10T00:00:00ZHandlehttps://hdl.handle.net/1822/41296http://purl.org/coar/access_right/c_abf2open accessBacterial CelluloseLaccaseAntimicrobialWound DressingImmobilizationCytotoxicity1804942 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/7d8c4543-fbf4-4cb9-abbd-b79d0195e7f7/download |
| spellingShingle | Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties Sampaio, Liliana M. P. Bacterial Cellulose Laccase Antimicrobial Wound Dressing Immobilization Cytotoxicity |
| status | SINGLETON |
| subject.fl_str_mv | Bacterial Cellulose Laccase Antimicrobial Wound Dressing Immobilization Cytotoxicity |
| title | Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties |
| title_full | Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties |
| title_fullStr | Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties |
| title_full_unstemmed | Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties |
| title_short | Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties |
| title_sort | Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties |
| topic | Bacterial Cellulose Laccase Antimicrobial Wound Dressing Immobilization Cytotoxicity |
| topic_facet | Bacterial Cellulose Laccase Antimicrobial Wound Dressing Immobilization Cytotoxicity |
| url | https://hdl.handle.net/1822/41296 |
| visible | 1 |