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Low cost microfluidic device for partial cell separation: micromilling approach

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Resumo:Several studies have already demonstrated that it is possible to perform blood flow studies in microfluidic systems fabricated by using low-cost techniques. However, most of these techniques do not produce microchannels smaller than 100 microns and as a result they have several limitations related to blood cell separation. Recently, manufacturers have been able to produce milling tools smaller than 100 microns, which consequently have promoted the ability of micromilling machines to fabricate microfluidic devices able to perform separation of red blood cells (RBCs) from plasma. In this work, we show the ability of a micromilling machine to manufacture microchannels with dimensions down to 30 microns. Additionally, we show for the first time the ability of the proposed microfluidic device to enhance the cell-free layer close to the walls, leading to perform partial separation of RBCs from plasma.
Autores principais:Lopes, Raquel
Outros Autores:Rodrigues, Raquel O.; Pinho, Diana; Garcia, Valdemar; Schütte, Helmut; Lima, Rui Alberto Madeira Macedo; Gassmann, Stefan
Assunto:Micromilling Microfluidic device Nonlithographic technique Red blood cells Cell-free layer
Ano:2015
País:Portugal
Tipo de documento:comunicação em conferência
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho
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author Lopes, Raquel
author2 Rodrigues, Raquel O.
Pinho, Diana
Garcia, Valdemar
Schütte, Helmut
Lima, Rui Alberto Madeira Macedo
Gassmann, Stefan
author2_role author
author
author
author
author
author
author_facet Lopes, Raquel
Rodrigues, Raquel O.
Pinho, Diana
Garcia, Valdemar
Schütte, Helmut
Lima, Rui Alberto Madeira Macedo
Gassmann, Stefan
author_role author
contributor_name_str_mv Universidade do Minho
country_str PT
creators_json_txt [{\"Person.name\":\"Lopes, Raquel\"},{\"Person.name\":\"Rodrigues, Raquel O.\"},{\"Person.name\":\"Pinho, Diana\"},{\"Person.name\":\"Garcia, Valdemar\"},{\"Person.name\":\"Schütte, Helmut\"},{\"Person.name\":\"Lima, Rui Alberto Madeira Macedo\"},{\"Person.name\":\"Gassmann, Stefan\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Universidade do Minho
datacite.creators.creator.creatorName.fl_str_mv Lopes, Raquel
Rodrigues, Raquel O.
Pinho, Diana
Garcia, Valdemar
Schütte, Helmut
Lima, Rui Alberto Madeira Macedo
Gassmann, Stefan
datacite.date.Accepted.fl_str_mv 2015-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2018-02-28T09:42:15Z
datacite.date.embargoed.fl_str_mv 2018-02-28T09:42:15Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv Micromilling
Microfluidic device
Nonlithographic technique
Red blood cells
Cell-free layer
datacite.titles.title.fl_str_mv Low cost microfluidic device for partial cell separation: micromilling approach
dc.contributor.none.fl_str_mv Universidade do Minho
dc.creator.none.fl_str_mv Lopes, Raquel
Rodrigues, Raquel O.
Pinho, Diana
Garcia, Valdemar
Schütte, Helmut
Lima, Rui Alberto Madeira Macedo
Gassmann, Stefan
dc.date.Accepted.fl_str_mv 2015-01-01T00:00:00Z
dc.date.available.fl_str_mv 2018-02-28T09:42:15Z
dc.date.embargoed.fl_str_mv 2018-02-28T09:42:15Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv https://hdl.handle.net/1822/51178
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv IEEE
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv Micromilling
Microfluidic device
Nonlithographic technique
Red blood cells
Cell-free layer
dc.title.fl_str_mv Low cost microfluidic device for partial cell separation: micromilling approach
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_5794
description Several studies have already demonstrated that it is possible to perform blood flow studies in microfluidic systems fabricated by using low-cost techniques. However, most of these techniques do not produce microchannels smaller than 100 microns and as a result they have several limitations related to blood cell separation. Recently, manufacturers have been able to produce milling tools smaller than 100 microns, which consequently have promoted the ability of micromilling machines to fabricate microfluidic devices able to perform separation of red blood cells (RBCs) from plasma. In this work, we show the ability of a micromilling machine to manufacture microchannels with dimensions down to 30 microns. Additionally, we show for the first time the ability of the proposed microfluidic device to enhance the cell-free layer close to the walls, leading to perform partial separation of RBCs from plasma.
dirty 0
eu_rights_str_mv openAccess
format conferencePaper
fulltext.url.fl_str_mv https://prod-dspace.uminho.pt/bitstreams/d963ddf3-11ae-4381-962a-146378a3a6ea/download
id rum_ee89bc2ebd8b111a08b5a4aa89a628ab
identifier.url.fl_str_mv https://hdl.handle.net/1822/51178
instacron_str repositorium
institution Universidade do Minho
instname_str Universidade do Minho
language eng
network_acronym_str rum
network_name_str RepositóriUM - Universidade do Minho
oai_identifier_str oai:repositorium.uminho.pt:1822/51178
organization_str_mv urn:organizationAcronym:repositorium
person_str_mv Lopes, Raquel
Rodrigues, Raquel O.
Pinho, Diana
Garcia, Valdemar
Schütte, Helmut
Lima, Rui Alberto Madeira Macedo
Gassmann, Stefan
publishDate 2015
publisher.none.fl_str_mv IEEE
reponame_str RepositóriUM - Universidade do Minho
repository_id_str urn:repositoryAcronym:rum
service_str_mv urn:repositoryAcronym:rum
spelling engIEEEporSeveral studies have already demonstrated that it is possible to perform blood flow studies in microfluidic systems fabricated by using low-cost techniques. However, most of these techniques do not produce microchannels smaller than 100 microns and as a result they have several limitations related to blood cell separation. Recently, manufacturers have been able to produce milling tools smaller than 100 microns, which consequently have promoted the ability of micromilling machines to fabricate microfluidic devices able to perform separation of red blood cells (RBCs) from plasma. In this work, we show the ability of a micromilling machine to manufacture microchannels with dimensions down to 30 microns. Additionally, we show for the first time the ability of the proposed microfluidic device to enhance the cell-free layer close to the walls, leading to perform partial separation of RBCs from plasma.application/pdfporLow cost microfluidic device for partial cell separation: micromilling approachLopes, RaquelRodrigues, Raquel O.Pinho, DianaGarcia, ValdemarSchütte, HelmutLima, Rui Alberto Madeira MacedoGassmann, StefanHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptDOIIsPartOf10.1109/ICIT.2015.71255942018-02-28T09:42:15Z20152015-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/51178http://purl.org/coar/access_right/c_abf2open accessMicromillingMicrofluidic deviceNonlithographic techniqueRed blood cellsCell-free layer1280606 bytesother research producthttp://purl.org/coar/resource_type/c_5794conference paperhttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/d963ddf3-11ae-4381-962a-146378a3a6ea/download
spellingShingle Low cost microfluidic device for partial cell separation: micromilling approach
Lopes, Raquel
Micromilling
Microfluidic device
Nonlithographic technique
Red blood cells
Cell-free layer
status SINGLETON
subject.fl_str_mv Micromilling
Microfluidic device
Nonlithographic technique
Red blood cells
Cell-free layer
title Low cost microfluidic device for partial cell separation: micromilling approach
title_full Low cost microfluidic device for partial cell separation: micromilling approach
title_fullStr Low cost microfluidic device for partial cell separation: micromilling approach
title_full_unstemmed Low cost microfluidic device for partial cell separation: micromilling approach
title_short Low cost microfluidic device for partial cell separation: micromilling approach
title_sort Low cost microfluidic device for partial cell separation: micromilling approach
topic Micromilling
Microfluidic device
Nonlithographic technique
Red blood cells
Cell-free layer
topic_facet Micromilling
Microfluidic device
Nonlithographic technique
Red blood cells
Cell-free layer
url https://hdl.handle.net/1822/51178
visible 1