Publicação
Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate
| Resumo: | Using lignocellulosic biomass hydrolysate as a renewable and abundant feedstock for microbial lipids production is a sustainable and economic high-potential approach. This study investigated the potential of the oleaginous yeast Yarrowia lipolytica to produce lipids-rich biomass from eucalyptus bark hydrolysate (EBH) obtained by enzymatic hydrolysis of the biomass pretreated by steam explosion. The effect of EBH concentration (undiluted and 1:3 v/v diluted) and medium supplementation (CSL and KH2PO4) was evaluated in Erlenmeyer flasks and lab-scale stirred tank bioreactor, respectively. Additionally, the effect of volumetric oxygen transfer coefficient (kLa) and mode of operation (batch and two-stage repeated batch) was also assessed in the bioreactor. Under the best experimental conditions (undiluted EBH, 2gL1 CSL, 1.8gL1(NH4)2SO4, and kLa of 66 h1), Y. lipolytica W29 grown in batch cultures accumulated 26 % (w/w) of intracellular lipids, corresponding to 5.6gL1 of concentration. Lipids of Y. lipolytica were highly unsaturated and mainly composed of oleic acid (48 %), followed by palmitoleic (20 %), linoleic (17 %) and palmitic acids (14 %). This composition of Y. lipolytica lipids suggests their potential use as feedstock for biodiesel (a renewable biofuel). This work demonstrated the robust features of Y. lipolytica W29 as a potential lipids production platform to implement lignocellulose-based biorefineries. |
|---|---|
| Autores principais: | Dias, Bruna |
| Outros Autores: | Lopes, Marlene; Fernandes, Helena; Marques, Susana; Gírio, Francisco; Belo, Isabel |
| Assunto: | Eucalyptus bark hydrolysate Microbial lipids Volumetric oxygen transfer coefficient Yarrowia lipolytica |
| Ano: | 2024 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866877454401929216 |
|---|---|
| author | Dias, Bruna |
| author2 | Lopes, Marlene Fernandes, Helena Marques, Susana Gírio, Francisco Belo, Isabel |
| author2_role | author author author author author |
| author_facet | Dias, Bruna Lopes, Marlene Fernandes, Helena Marques, Susana Gírio, Francisco Belo, Isabel |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Dias, Bruna\"},{\"Person.name\":\"Lopes, Marlene\"},{\"Person.name\":\"Fernandes, Helena\"},{\"Person.name\":\"Marques, Susana\"},{\"Person.name\":\"Gírio, Francisco\"},{\"Person.name\":\"Belo, Isabel\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Dias, Bruna Lopes, Marlene Fernandes, Helena Marques, Susana Gírio, Francisco Belo, Isabel |
| datacite.date.Accepted.fl_str_mv | 2024-04-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2024-03-12T17:51:56Z |
| datacite.date.embargoed.fl_str_mv | 2024-03-12T17:51:56Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Eucalyptus bark hydrolysate Microbial lipids Volumetric oxygen transfer coefficient Yarrowia lipolytica |
| datacite.titles.title.fl_str_mv | Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Dias, Bruna Lopes, Marlene Fernandes, Helena Marques, Susana Gírio, Francisco Belo, Isabel |
| dc.date.Accepted.fl_str_mv | 2024-04-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2024-03-12T17:51:56Z |
| dc.date.embargoed.fl_str_mv | 2024-03-12T17:51:56Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/89459 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.rights.rights.copyright.fl_str_mv | openAccess |
| dc.subject.none.fl_str_mv | Eucalyptus bark hydrolysate Microbial lipids Volumetric oxygen transfer coefficient Yarrowia lipolytica |
| dc.title.fl_str_mv | Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Using lignocellulosic biomass hydrolysate as a renewable and abundant feedstock for microbial lipids production is a sustainable and economic high-potential approach. This study investigated the potential of the oleaginous yeast Yarrowia lipolytica to produce lipids-rich biomass from eucalyptus bark hydrolysate (EBH) obtained by enzymatic hydrolysis of the biomass pretreated by steam explosion. The effect of EBH concentration (undiluted and 1:3 v/v diluted) and medium supplementation (CSL and KH2PO4) was evaluated in Erlenmeyer flasks and lab-scale stirred tank bioreactor, respectively. Additionally, the effect of volumetric oxygen transfer coefficient (kLa) and mode of operation (batch and two-stage repeated batch) was also assessed in the bioreactor. Under the best experimental conditions (undiluted EBH, 2gL1 CSL, 1.8gL1(NH4)2SO4, and kLa of 66 h1), Y. lipolytica W29 grown in batch cultures accumulated 26 % (w/w) of intracellular lipids, corresponding to 5.6gL1 of concentration. Lipids of Y. lipolytica were highly unsaturated and mainly composed of oleic acid (48 %), followed by palmitoleic (20 %), linoleic (17 %) and palmitic acids (14 %). This composition of Y. lipolytica lipids suggests their potential use as feedstock for biodiesel (a renewable biofuel). This work demonstrated the robust features of Y. lipolytica W29 as a potential lipids production platform to implement lignocellulose-based biorefineries. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/1bbdca84-a0bf-4a5e-835e-00c32a47436c/download |
| id | rum_eef3f7f2df9c43dd8a99c2aed2bddfb9 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/89459 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/89459 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Dias, Bruna Lopes, Marlene Fernandes, Helena Marques, Susana Gírio, Francisco Belo, Isabel |
| publishDate | 2024 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engElsevierporUsing lignocellulosic biomass hydrolysate as a renewable and abundant feedstock for microbial lipids production is a sustainable and economic high-potential approach. This study investigated the potential of the oleaginous yeast Yarrowia lipolytica to produce lipids-rich biomass from eucalyptus bark hydrolysate (EBH) obtained by enzymatic hydrolysis of the biomass pretreated by steam explosion. The effect of EBH concentration (undiluted and 1:3 v/v diluted) and medium supplementation (CSL and KH2PO4) was evaluated in Erlenmeyer flasks and lab-scale stirred tank bioreactor, respectively. Additionally, the effect of volumetric oxygen transfer coefficient (kLa) and mode of operation (batch and two-stage repeated batch) was also assessed in the bioreactor. Under the best experimental conditions (undiluted EBH, 2gL1 CSL, 1.8gL1(NH4)2SO4, and kLa of 66 h1), Y. lipolytica W29 grown in batch cultures accumulated 26 % (w/w) of intracellular lipids, corresponding to 5.6gL1 of concentration. Lipids of Y. lipolytica were highly unsaturated and mainly composed of oleic acid (48 %), followed by palmitoleic (20 %), linoleic (17 %) and palmitic acids (14 %). This composition of Y. lipolytica lipids suggests their potential use as feedstock for biodiesel (a renewable biofuel). This work demonstrated the robust features of Y. lipolytica W29 as a potential lipids production platform to implement lignocellulose-based biorefineries.application/pdfporBiomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysateDias, BrunaLopes, MarleneFernandes, HelenaMarques, SusanaGírio, FranciscoBelo, IsabelHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf0960-1481DOIIsPartOf10.1016/j.renene.2024.1201732024-03-12T17:51:56Z2024-042024-03-09T10:29:27Z2024-04-01T00:00:00ZHandlehttps://hdl.handle.net/1822/89459http://purl.org/coar/access_right/c_abf2open accessEucalyptus bark hydrolysateMicrobial lipidsVolumetric oxygen transfer coefficientYarrowia lipolytica992667 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2024-04http://creativecommons.org/licenses/by-nc-nd/4.0/openAccesshttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/1bbdca84-a0bf-4a5e-835e-00c32a47436c/download |
| spellingShingle | Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate Dias, Bruna Eucalyptus bark hydrolysate Microbial lipids Volumetric oxygen transfer coefficient Yarrowia lipolytica |
| status | SINGLETON |
| subject.fl_str_mv | Eucalyptus bark hydrolysate Microbial lipids Volumetric oxygen transfer coefficient Yarrowia lipolytica |
| title | Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate |
| title_full | Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate |
| title_fullStr | Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate |
| title_full_unstemmed | Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate |
| title_short | Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate |
| title_sort | Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate |
| topic | Eucalyptus bark hydrolysate Microbial lipids Volumetric oxygen transfer coefficient Yarrowia lipolytica |
| topic_facet | Eucalyptus bark hydrolysate Microbial lipids Volumetric oxygen transfer coefficient Yarrowia lipolytica |
| url | https://hdl.handle.net/1822/89459 |
| visible | 1 |