Publicação
Treatment of wool fibres with subtilisin and subtilisin-PEG
| Resumo: | In this work the diffusion of serine proteases into wool fabrics and yarns was studied. The proteases used were free subtilisin and subtilisin-PEG (the same enzyme that was covalently cross linked to polyethylene glycol). It is shown that the adsorption and diffusion is facilitated by the pre-treatment performed, being the alkaline surfactant washing and bleaching the most effective in what concerns enzyme adsorption. Furthermore, this study suggests that the diffusion of proteases into wool is dependent on the size of the protease. The free enzyme penetrates into wool fibre cortex while the modified bigger enzyme is retained only at the surface, in the cuticle layer. Also, proteins without proteolytic activity do not adsorb considerably on wool due to its hydrophobic nature, therefore the diffusion is facilitated by hydrolytic action. These results have important practical implications for the establishment of enzymatic wool finishing processes, since they allow for control of the enzyme hydrolysis, which was the major drawback of this environmental friendly option to the conventional chlorine treatments. |
|---|---|
| Autores principais: | Silva, Carla J. S. M. |
| Outros Autores: | Prabaharan, M.; Gübitz, Georg M.; Paulo, Artur Cavaco |
| Assunto: | Serine proteases Protein adsorption Wool fibre Clean technology Cross linking wool fibres clean technology |
| Ano: | 2005 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866875450780811264 |
|---|---|
| author | Silva, Carla J. S. M. |
| author2 | Prabaharan, M. Gübitz, Georg M. Paulo, Artur Cavaco |
| author2_role | author author author |
| author_facet | Silva, Carla J. S. M. Prabaharan, M. Gübitz, Georg M. Paulo, Artur Cavaco |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Silva, Carla J. S. M.\"},{\"Person.name\":\"Prabaharan, M.\"},{\"Person.name\":\"Gübitz, Georg M.\"},{\"Person.name\":\"Paulo, Artur Cavaco\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Silva, Carla J. S. M. Prabaharan, M. Gübitz, Georg M. Paulo, Artur Cavaco |
| datacite.date.Accepted.fl_str_mv | 2005-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2005-08-26T14:43:04Z |
| datacite.date.embargoed.fl_str_mv | 2005-08-26T14:43:04Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Serine proteases Protein adsorption Wool fibre Clean technology Cross linking wool fibres clean technology |
| datacite.titles.title.fl_str_mv | Treatment of wool fibres with subtilisin and subtilisin-PEG |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Silva, Carla J. S. M. Prabaharan, M. Gübitz, Georg M. Paulo, Artur Cavaco |
| dc.date.Accepted.fl_str_mv | 2005-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2005-08-26T14:43:04Z |
| dc.date.embargoed.fl_str_mv | 2005-08-26T14:43:04Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/2655 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Serine proteases Protein adsorption Wool fibre Clean technology Cross linking wool fibres clean technology |
| dc.title.fl_str_mv | Treatment of wool fibres with subtilisin and subtilisin-PEG |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | In this work the diffusion of serine proteases into wool fabrics and yarns was studied. The proteases used were free subtilisin and subtilisin-PEG (the same enzyme that was covalently cross linked to polyethylene glycol). It is shown that the adsorption and diffusion is facilitated by the pre-treatment performed, being the alkaline surfactant washing and bleaching the most effective in what concerns enzyme adsorption. Furthermore, this study suggests that the diffusion of proteases into wool is dependent on the size of the protease. The free enzyme penetrates into wool fibre cortex while the modified bigger enzyme is retained only at the surface, in the cuticle layer. Also, proteins without proteolytic activity do not adsorb considerably on wool due to its hydrophobic nature, therefore the diffusion is facilitated by hydrolytic action. These results have important practical implications for the establishment of enzymatic wool finishing processes, since they allow for control of the enzyme hydrolysis, which was the major drawback of this environmental friendly option to the conventional chlorine treatments. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/0b56bda0-bfa9-4a67-b73d-2e8fcb04c1f4/download |
| id | rum_f19087ba48d18afa26e8d6844e369d56 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/2655 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/2655 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Silva, Carla J. S. M. Prabaharan, M. Gübitz, Georg M. Paulo, Artur Cavaco |
| publishDate | 2005 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engElsevierengIn this work the diffusion of serine proteases into wool fabrics and yarns was studied. The proteases used were free subtilisin and subtilisin-PEG (the same enzyme that was covalently cross linked to polyethylene glycol). It is shown that the adsorption and diffusion is facilitated by the pre-treatment performed, being the alkaline surfactant washing and bleaching the most effective in what concerns enzyme adsorption. Furthermore, this study suggests that the diffusion of proteases into wool is dependent on the size of the protease. The free enzyme penetrates into wool fibre cortex while the modified bigger enzyme is retained only at the surface, in the cuticle layer. Also, proteins without proteolytic activity do not adsorb considerably on wool due to its hydrophobic nature, therefore the diffusion is facilitated by hydrolytic action. These results have important practical implications for the establishment of enzymatic wool finishing processes, since they allow for control of the enzyme hydrolysis, which was the major drawback of this environmental friendly option to the conventional chlorine treatments.application/pdfengTreatment of wool fibres with subtilisin and subtilisin-PEGSilva, Carla J. S. M.Prabaharan, M.Gübitz, Georg M.Paulo, Artur CavacoHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf0141-0229DOIIsPartOf10.1016/j.enzmictec.2005.01.0172005-08-26T14:43:04Z200520052005-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/2655http://purl.org/coar/access_right/c_abf2open accessSerine proteasesProtein adsorptionWool fibreClean technologyCross linkingwool fibres clean technology315115 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/0b56bda0-bfa9-4a67-b73d-2e8fcb04c1f4/download |
| spellingShingle | Treatment of wool fibres with subtilisin and subtilisin-PEG Silva, Carla J. S. M. Serine proteases Protein adsorption Wool fibre Clean technology Cross linking wool fibres clean technology |
| status | SINGLETON |
| subject.fl_str_mv | Serine proteases Protein adsorption Wool fibre Clean technology Cross linking wool fibres clean technology |
| title | Treatment of wool fibres with subtilisin and subtilisin-PEG |
| title_full | Treatment of wool fibres with subtilisin and subtilisin-PEG |
| title_fullStr | Treatment of wool fibres with subtilisin and subtilisin-PEG |
| title_full_unstemmed | Treatment of wool fibres with subtilisin and subtilisin-PEG |
| title_short | Treatment of wool fibres with subtilisin and subtilisin-PEG |
| title_sort | Treatment of wool fibres with subtilisin and subtilisin-PEG |
| topic | Serine proteases Protein adsorption Wool fibre Clean technology Cross linking wool fibres clean technology |
| topic_facet | Serine proteases Protein adsorption Wool fibre Clean technology Cross linking wool fibres clean technology |
| url | https://hdl.handle.net/1822/2655 |
| visible | 1 |